Diet-derived short chain fatty acids stimulate intestinal epithelial cells to induce mucosal tolerogenic dendritic cells

Gera Goverse, Rosalie Molenaar, Laurence MacIa, Jian Tan, Martje N. Erkelens, Tanja Konijn, Marlene Knippenberg, Emma C.L. Cook, Diana Hanekamp, Marc Veldhoen, Anita Hartog, Guus Roeselers, Charles R. MacKay, Reina E. Mebius*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The gastrointestinal tract is continuously exposed to many environmental factors that influence intestinal epithelial cells and the underlying mucosal immune system. In this article, we demonstrate that dietary fiber and short chain fatty acids (SCFAs) induced the expression of the Vitamin A-converting enzyme RALDH1 in intestinal epithelial cells in vivo and in vitro, respectively. Furthermore, our data showed that the expression levels of RALDH1 in small intestinal epithelial cells correlated with the activity of Vitamin A-converting enzymes in mesenteric lymph node dendritic cells, along with increased numbers of intestinal regulatory T cells and a higher production of luminal IgA. Moreover, we show that the consumption of dietary fiber can alter the composition of SCFA-producing microbiota and SCFA production in the small intestines. In conclusion, our data illustrate that dietary adjustments affect small intestinal epithelial cells and can be used to modulate the mucosal immune system.

Original languageEnglish
Pages (from-to)2172-2181
Number of pages10
JournalJournal of Immunology
Volume198
Issue number5
DOIs
Publication statusPublished - 1 Mar 2017

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