Differential Maturation of the Two Regulated Secretory Pathways in Human iPSC-Derived Neurons

Javier Emperador Melero, Aishwarya G. Nadadhur, Desiree Schut, Jan V. Weering, Vivi M. Heine, Ruud F. Toonen, Matthijs Verhage

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Neurons communicate by regulated secretion of chemical signals from synaptic vesicles (SVs) and dense-core vesicles (DCVs). Here, we investigated the maturation of these two secretory pathways in micro-networks of human iPSC-derived neurons. These micro-networks abundantly expressed endogenous SV and DCV markers, including neuropeptides. DCV transport was microtubule dependent, preferentially anterograde in axons, and 2-fold faster in axons than in dendrites. SV and DCV secretion were strictly Ca2+ and SNARE dependent. DCV secretion capacity matured until day in vitro (DIV) 36, with intense stimulation releasing 6% of the total DCV pool, and then plateaued. This efficiency is comparable with mature mouse neurons. In contrast, SV secretion capacity continued to increase until DIV50, with substantial further increase in secretion efficiency and decrease in silent synapses. These data show that the two secretory pathways can be studied in human neurons and that they mature differentially, with DCV secretion reaching maximum efficiency when that of SVs is still low.

Original languageEnglish
Pages (from-to)659-672
Number of pages14
JournalStem Cell Reports
Volume8
Issue number3
DOIs
Publication statusPublished - 14 Mar 2017

Cite this

Emperador Melero, Javier ; Nadadhur, Aishwarya G. ; Schut, Desiree ; Weering, Jan V. ; Heine, Vivi M. ; Toonen, Ruud F. ; Verhage, Matthijs. / Differential Maturation of the Two Regulated Secretory Pathways in Human iPSC-Derived Neurons. In: Stem Cell Reports. 2017 ; Vol. 8, No. 3. pp. 659-672.
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abstract = "Neurons communicate by regulated secretion of chemical signals from synaptic vesicles (SVs) and dense-core vesicles (DCVs). Here, we investigated the maturation of these two secretory pathways in micro-networks of human iPSC-derived neurons. These micro-networks abundantly expressed endogenous SV and DCV markers, including neuropeptides. DCV transport was microtubule dependent, preferentially anterograde in axons, and 2-fold faster in axons than in dendrites. SV and DCV secretion were strictly Ca2+ and SNARE dependent. DCV secretion capacity matured until day in vitro (DIV) 36, with intense stimulation releasing 6{\%} of the total DCV pool, and then plateaued. This efficiency is comparable with mature mouse neurons. In contrast, SV secretion capacity continued to increase until DIV50, with substantial further increase in secretion efficiency and decrease in silent synapses. These data show that the two secretory pathways can be studied in human neurons and that they mature differentially, with DCV secretion reaching maximum efficiency when that of SVs is still low.",
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Differential Maturation of the Two Regulated Secretory Pathways in Human iPSC-Derived Neurons. / Emperador Melero, Javier; Nadadhur, Aishwarya G.; Schut, Desiree; Weering, Jan V.; Heine, Vivi M.; Toonen, Ruud F.; Verhage, Matthijs.

In: Stem Cell Reports, Vol. 8, No. 3, 14.03.2017, p. 659-672.

Research output: Contribution to journalArticleAcademicpeer-review

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