TY - JOUR
T1 - Differential transcriptional profile through cell cycle progression in Arabidopsis cultures under simulated microgravity
AU - Kamal, Khaled Y.
AU - van Loon, Jack J. W. A.
AU - Medina, F. Javier
AU - Herranz, Raúl
PY - 2019
Y1 - 2019
N2 - Plant cell proliferation is affected by microgravity during spaceflight, but involved molecular mechanisms, key for space agronomy goals, remain unclear. To investigate transcriptomic changes in cell cycle phases caused by simulated microgravity, an Arabidopsis immobilized synchronous suspension culture was incubated in a Random Positioning Machine. After simulation, a transcriptomic analysis was performed with two subpopulations of cells (G2/M and G1 phases enriched) and an asynchronous culture sample. Differential expression was found at cell proliferation, energy/redox and stress responses, plus unknown biological processes gene ontology groups. Overall expression inhibition was a common response to simulated microgravity, but differences peak at the G2/M phase and stress response components change dramatically from G2/M to the G1 subpopulation suggesting a differential adaptation response to simulated microgravity through the cell cycle. Cell cycle adaptation using both known stress mechanisms and unknown function genes may cope with reduced gravity as an evolutionary novel environment.
AB - Plant cell proliferation is affected by microgravity during spaceflight, but involved molecular mechanisms, key for space agronomy goals, remain unclear. To investigate transcriptomic changes in cell cycle phases caused by simulated microgravity, an Arabidopsis immobilized synchronous suspension culture was incubated in a Random Positioning Machine. After simulation, a transcriptomic analysis was performed with two subpopulations of cells (G2/M and G1 phases enriched) and an asynchronous culture sample. Differential expression was found at cell proliferation, energy/redox and stress responses, plus unknown biological processes gene ontology groups. Overall expression inhibition was a common response to simulated microgravity, but differences peak at the G2/M phase and stress response components change dramatically from G2/M to the G1 subpopulation suggesting a differential adaptation response to simulated microgravity through the cell cycle. Cell cycle adaptation using both known stress mechanisms and unknown function genes may cope with reduced gravity as an evolutionary novel environment.
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85060106311&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/30641127
U2 - 10.1016/j.ygeno.2019.01.007
DO - 10.1016/j.ygeno.2019.01.007
M3 - Article
C2 - 30641127
VL - 111
SP - 1956
EP - 1965
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 6
ER -