Differentially expressed transcripts in X-ray-induced lymphomas identified by dioxygenin-labeled differential display

C. Willemien Van Der Houven Van Oordt, Theo G. Schouten, Alex J. Van Der Eb, Marco L. Breuer*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Differential display is an easily applied method for comparing gene expression in a variety of systems. We used a nonradioactive differential display technique to analyze X-ray-induced lymphomas derived from Eμ-pim-1 transgenic and nontransgenic mice. Fragments of 11 differentially regulated genes were identified, three of which are novel sequences. One of the cloned fragments contained sequences of a mouse VL30 retro-element that was significantly overexpressed in a subset of lymphomas as compared with non- lymphomatous tissue. Interestingly, these lymphomas also displayed high levels of c-myc transcripts. An altered expression pattern of a glutathione S-transferase homologue was identified in several lymphomas. Moreover, a cytotoxic T-lymphocyte lipase appeared to be overexpressed specifically in lymphoma-containing spleen tissue, and the results suggest that it may be related to the endogenous immune response against lymphoma development.

Original languageEnglish
Pages (from-to)29-35
Number of pages7
JournalMolecular Carcinogenesis
Volume24
Issue number1
DOIs
Publication statusPublished - 1999
Externally publishedYes

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