Distribution, location, and transcriptional profile of Peyer's patch conventional DC subsets at steady state and under TLR7 ligand stimulation

J. Bonnardel, C. Da Silva, C. Wagner, R. Bonifay, L. Chasson, M. Masse, E. Pollet, M. Dalod, J. P. Gorvel, H. Lelouard

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The initiation of the mucosal immune response in Peyer's patch (PP) relies on the sampling, processing, and efficient presentation of foreign antigens by dendritic cells (DCs). Among PP DCs, CD11b + conventional DCs (cDCs) and lysozyme-expressing DCs (LysoDCs) have distinct progenitors and functions but share many cell surface markers. This has previously led to confusion between these two subsets. In addition, another PP DC subset, termed double-negative (DN), remains poorly characterized. Here we show that both DN and CD11b + cDCs belong to a unique SIRPα + cDC subset. At steady state, cDCs and TIM-4 + macrophages are mainly located in T-cell zones, i.e., interfollicular regions, whereas a majority of subepithelial phagocytes are monocyte-derived cells, namely, LysoDCs and TIM-4 ' macrophages. Finally, oral administration of a Toll-like receptor 7 ligand induces at least three TNF-dependent events: (i) migration of dome-associated villus cDCs in interfollicular regions, (ii) increase of CD8α + interfollicular cDC number, and (iii) activation of both CD11b + and CD8α + interfollicular cDCs. The latter is marked by a genetic reprograming leading to the upregulation of type I interferon-stimulated and of both immuno-stimulatory and -inhibitory gene expression.

Original languageEnglish
Pages (from-to)1412-1430
Number of pages19
JournalMucosal Immunology
Volume10
Issue number6
DOIs
Publication statusPublished - 1 Nov 2017

Cite this

Bonnardel, J. ; Da Silva, C. ; Wagner, C. ; Bonifay, R. ; Chasson, L. ; Masse, M. ; Pollet, E. ; Dalod, M. ; Gorvel, J. P. ; Lelouard, H. / Distribution, location, and transcriptional profile of Peyer's patch conventional DC subsets at steady state and under TLR7 ligand stimulation. In: Mucosal Immunology. 2017 ; Vol. 10, No. 6. pp. 1412-1430.
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abstract = "The initiation of the mucosal immune response in Peyer's patch (PP) relies on the sampling, processing, and efficient presentation of foreign antigens by dendritic cells (DCs). Among PP DCs, CD11b + conventional DCs (cDCs) and lysozyme-expressing DCs (LysoDCs) have distinct progenitors and functions but share many cell surface markers. This has previously led to confusion between these two subsets. In addition, another PP DC subset, termed double-negative (DN), remains poorly characterized. Here we show that both DN and CD11b + cDCs belong to a unique SIRPα + cDC subset. At steady state, cDCs and TIM-4 + macrophages are mainly located in T-cell zones, i.e., interfollicular regions, whereas a majority of subepithelial phagocytes are monocyte-derived cells, namely, LysoDCs and TIM-4 ' macrophages. Finally, oral administration of a Toll-like receptor 7 ligand induces at least three TNF-dependent events: (i) migration of dome-associated villus cDCs in interfollicular regions, (ii) increase of CD8α + interfollicular cDC number, and (iii) activation of both CD11b + and CD8α + interfollicular cDCs. The latter is marked by a genetic reprograming leading to the upregulation of type I interferon-stimulated and of both immuno-stimulatory and -inhibitory gene expression.",
author = "J. Bonnardel and {Da Silva}, C. and C. Wagner and R. Bonifay and L. Chasson and M. Masse and E. Pollet and M. Dalod and Gorvel, {J. P.} and H. Lelouard",
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Bonnardel, J, Da Silva, C, Wagner, C, Bonifay, R, Chasson, L, Masse, M, Pollet, E, Dalod, M, Gorvel, JP & Lelouard, H 2017, 'Distribution, location, and transcriptional profile of Peyer's patch conventional DC subsets at steady state and under TLR7 ligand stimulation' Mucosal Immunology, vol. 10, no. 6, pp. 1412-1430. https://doi.org/10.1038/mi.2017.30

Distribution, location, and transcriptional profile of Peyer's patch conventional DC subsets at steady state and under TLR7 ligand stimulation. / Bonnardel, J.; Da Silva, C.; Wagner, C.; Bonifay, R.; Chasson, L.; Masse, M.; Pollet, E.; Dalod, M.; Gorvel, J. P.; Lelouard, H.

In: Mucosal Immunology, Vol. 10, No. 6, 01.11.2017, p. 1412-1430.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Bonnardel, J.

AU - Da Silva, C.

AU - Wagner, C.

AU - Bonifay, R.

AU - Chasson, L.

AU - Masse, M.

AU - Pollet, E.

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AU - Gorvel, J. P.

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