Effect of long-term storage in biobanks on cerebrospinal fluid biomarker Aβ1-42, T-tau, and P-tau values

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Abstract

Introduction We studied the effect of long-term storage at −80°C on cerebrospinal fluid (CSF) biomarker levels. Our approach assumed consistency of mean biomarker levels in a homogenous Alzheimer's disease patient cohort over time. Methods We selected 148 Alzheimer's disease samples that had inclusion dates equally distributed over the years 2001 to 2013 from our biobank. The concentrations of CSF biomarkers, amyloid β1–42 (Aβ1–42), total tau (T-tau), and phosphorylated tau181 (P-tau), were measured with one enzyme-linked immunosorbent assay lot. Results were compared with historical results obtained at biobank inclusion. Results Linear regression analyses showed that the levels of CSF biomarkers, Aβ1–42, T-tau, and P-tau, were not related to storage time at −80°C (β = 0.015, 0.048, and 0.0016 pg/mL per day, not significant). However, the differences between remeasured concentrations of Aβ1–42 and concentrations at biobank inclusion measured for more than 30 assay batches increased with increasing time difference. Discussion The levels of CSF biomarkers, Aβ1–42, T-tau, and P-tau, did not significantly change during the maximum period of 12 years of storage at −80°C. Batch variation for Aβ1–42 is a factor that should be controlled for when using historical cohorts.

Original languageEnglish
Pages (from-to)45-50
Number of pages6
JournalAlzheimer's and Dementia: Diagnosis, Assessment and Disease Monitoring
Volume8
DOIs
Publication statusPublished - 2017

Cite this

@article{d2f646d49adb4ebdb20da74b8cee151e,
title = "Effect of long-term storage in biobanks on cerebrospinal fluid biomarker Aβ1-42, T-tau, and P-tau values",
abstract = "Introduction We studied the effect of long-term storage at −80°C on cerebrospinal fluid (CSF) biomarker levels. Our approach assumed consistency of mean biomarker levels in a homogenous Alzheimer's disease patient cohort over time. Methods We selected 148 Alzheimer's disease samples that had inclusion dates equally distributed over the years 2001 to 2013 from our biobank. The concentrations of CSF biomarkers, amyloid β1–42 (Aβ1–42), total tau (T-tau), and phosphorylated tau181 (P-tau), were measured with one enzyme-linked immunosorbent assay lot. Results were compared with historical results obtained at biobank inclusion. Results Linear regression analyses showed that the levels of CSF biomarkers, Aβ1–42, T-tau, and P-tau, were not related to storage time at −80°C (β = 0.015, 0.048, and 0.0016 pg/mL per day, not significant). However, the differences between remeasured concentrations of Aβ1–42 and concentrations at biobank inclusion measured for more than 30 assay batches increased with increasing time difference. Discussion The levels of CSF biomarkers, Aβ1–42, T-tau, and P-tau, did not significantly change during the maximum period of 12 years of storage at −80°C. Batch variation for Aβ1–42 is a factor that should be controlled for when using historical cohorts.",
keywords = "Alzheimer's disease, Amyloid β, Batch variation, Biomarkers, Cerebrospinal fluid, ELISA, Long-term storage, Phosphorylated tau, Preanalytical variation, Total tau",
author = "Willemse, {Eline A.J.} and {van Uffelen}, {Kees W.J.} and {van der Flier}, {Wiesje M.} and Teunissen, {Charlotte E.}",
year = "2017",
doi = "10.1016/j.dadm.2017.03.005",
language = "English",
volume = "8",
pages = "45--50",
journal = "Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring",
issn = "2352-8729",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Effect of long-term storage in biobanks on cerebrospinal fluid biomarker Aβ1-42, T-tau, and P-tau values

AU - Willemse, Eline A.J.

AU - van Uffelen, Kees W.J.

AU - van der Flier, Wiesje M.

AU - Teunissen, Charlotte E.

PY - 2017

Y1 - 2017

N2 - Introduction We studied the effect of long-term storage at −80°C on cerebrospinal fluid (CSF) biomarker levels. Our approach assumed consistency of mean biomarker levels in a homogenous Alzheimer's disease patient cohort over time. Methods We selected 148 Alzheimer's disease samples that had inclusion dates equally distributed over the years 2001 to 2013 from our biobank. The concentrations of CSF biomarkers, amyloid β1–42 (Aβ1–42), total tau (T-tau), and phosphorylated tau181 (P-tau), were measured with one enzyme-linked immunosorbent assay lot. Results were compared with historical results obtained at biobank inclusion. Results Linear regression analyses showed that the levels of CSF biomarkers, Aβ1–42, T-tau, and P-tau, were not related to storage time at −80°C (β = 0.015, 0.048, and 0.0016 pg/mL per day, not significant). However, the differences between remeasured concentrations of Aβ1–42 and concentrations at biobank inclusion measured for more than 30 assay batches increased with increasing time difference. Discussion The levels of CSF biomarkers, Aβ1–42, T-tau, and P-tau, did not significantly change during the maximum period of 12 years of storage at −80°C. Batch variation for Aβ1–42 is a factor that should be controlled for when using historical cohorts.

AB - Introduction We studied the effect of long-term storage at −80°C on cerebrospinal fluid (CSF) biomarker levels. Our approach assumed consistency of mean biomarker levels in a homogenous Alzheimer's disease patient cohort over time. Methods We selected 148 Alzheimer's disease samples that had inclusion dates equally distributed over the years 2001 to 2013 from our biobank. The concentrations of CSF biomarkers, amyloid β1–42 (Aβ1–42), total tau (T-tau), and phosphorylated tau181 (P-tau), were measured with one enzyme-linked immunosorbent assay lot. Results were compared with historical results obtained at biobank inclusion. Results Linear regression analyses showed that the levels of CSF biomarkers, Aβ1–42, T-tau, and P-tau, were not related to storage time at −80°C (β = 0.015, 0.048, and 0.0016 pg/mL per day, not significant). However, the differences between remeasured concentrations of Aβ1–42 and concentrations at biobank inclusion measured for more than 30 assay batches increased with increasing time difference. Discussion The levels of CSF biomarkers, Aβ1–42, T-tau, and P-tau, did not significantly change during the maximum period of 12 years of storage at −80°C. Batch variation for Aβ1–42 is a factor that should be controlled for when using historical cohorts.

KW - Alzheimer's disease

KW - Amyloid β

KW - Batch variation

KW - Biomarkers

KW - Cerebrospinal fluid

KW - ELISA

KW - Long-term storage

KW - Phosphorylated tau

KW - Preanalytical variation

KW - Total tau

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U2 - 10.1016/j.dadm.2017.03.005

DO - 10.1016/j.dadm.2017.03.005

M3 - Article

VL - 8

SP - 45

EP - 50

JO - Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring

JF - Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring

SN - 2352-8729

ER -