Effect of single dose irradiation on human glioblastoma spheroids in vitro

Fabian Fehlauer, Lukas J A Stalpers, John Panayiotides, Patricia Kaaijk, Dionisio González González, Sieger Leenstra, Paul van der Valk, Peter Sminia

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Investigation of the predictive value of a radiosurgery-relevant treatment of glioblastoma spheroids. Organotypic multicellular spheroids were cultured and irradiated (20 Gy). Morphology, apoptosis and immunohistochemical expression of p53, p21, MIB-1, TGF-beta and VEGF were examined 4 h, 24 h, 7 days, and 14 days following treatment. Cell proliferation decreased, while apoptosis was increased. No morphological damage was observed. p53 expression was significantly increased after 4 h. TGF-beta and VEGF expression were only slightly altered. Particularly early changes in proliferation and apoptosis can be observed in spheroids. Individual response differences suggest spheroids of human gliomas to be useful for monitoring radiosurgery effects.

Original languageEnglish
Pages (from-to)477-85
Number of pages9
JournalOncology Reports
Issue number2
Publication statusPublished - Feb 2004

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