The effect of the number of two-wheeled containers at a gathering point on the energetic workload and the work efficiency in refuse collecting was studied in order to design an optimal gathering point for two-wheeled containers. Three sizes of gathering points were investigated, i.e. with 2, 16 and 32 two-wheeled containers at a gathering point. The collecting of two-wheeled containers was simulated in a test circuit. The energetic workload was quantified by the parameters oxygen uptake (lmin-1), heart rate (beatsmin-1) and perceived exertion. The work efficiency was quantified as the time it took to collect 32 two-wheeled containers per time period. The maximum acceptable amount of two-wheeled containers collected during an 8-h working day was estimated using the energetic criterion of a maximum oxygen uptake of 30% VO2max. The size of the gathering point had no effect on the oxygen uptake, heart rate or perceived exertion. However, the number of two-wheeled containers per collecting period (work efficiency) and the maximum acceptable amount during an 8-h working day were higher in the conditions with 16 and 32 two-wheeled containers at a gathering point compared to the condition with the 2 two-wheeled containers at a gathering point.