Effects of brain death and hemodynamic status on function and immunologic activation of the potential donor liver in the rat

Joost A.B. Van Der Hoeven, Gert J. Ter Horst, Grietje Molema, Paul De Vos, Armand R.J. Girbes, Folkert Postema, Reni L. Freund, Janneke Wiersema, Reinout Van Schilfgaarde, Rutger J. Ploeg

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Objective: To assess the effect on the function and immunologic status of potential donor livers of the duration of brain death combined with the presence and absence of hemodynamic instability in the donor. Summary Background Data: Brain death, regarded as a given condition in organ transplantation, could have significant effects on the donor organ quality. Methods: Brain death was induced in Wistar rats. Short or long periods of brain death in the presence or absence of hemodynamic instability were applied. Sham-operated rats served as controis. Organ function was studied by monitoring standard serum parameters. The inflammatory status of the liver was assessed by determining the immediate early gene products, the expression of cell adhesion molecules, and the influx of leukocytes in the liver. Results: Progressive organ dysfunction was most pronounced in hemodynamically unstable brain-dead donors. Irrespective of hemodynamic status, a progressive inflammatory activation could be observed in brain-dead rats compared with controls. Conclusions: Brain death causes progressive liver dysfunction, which is made worse by the coexistence of hemodynamic instability. Further, brain death activates the inflammatory status of the potential donor liver, irrespective of the presence of hypotension. The changes observed may predispose the graft to additional damage from ischemia and reperfusion in the transplant procedure.

Original languageEnglish
Pages (from-to)804-813
Number of pages10
JournalAnnals of Surgery
Volume232
Issue number6
DOIs
Publication statusPublished - 13 Dec 2000

Cite this

Van Der Hoeven, Joost A.B. ; Ter Horst, Gert J. ; Molema, Grietje ; De Vos, Paul ; Girbes, Armand R.J. ; Postema, Folkert ; Freund, Reni L. ; Wiersema, Janneke ; Van Schilfgaarde, Reinout ; Ploeg, Rutger J. / Effects of brain death and hemodynamic status on function and immunologic activation of the potential donor liver in the rat. In: Annals of Surgery. 2000 ; Vol. 232, No. 6. pp. 804-813.
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title = "Effects of brain death and hemodynamic status on function and immunologic activation of the potential donor liver in the rat",
abstract = "Objective: To assess the effect on the function and immunologic status of potential donor livers of the duration of brain death combined with the presence and absence of hemodynamic instability in the donor. Summary Background Data: Brain death, regarded as a given condition in organ transplantation, could have significant effects on the donor organ quality. Methods: Brain death was induced in Wistar rats. Short or long periods of brain death in the presence or absence of hemodynamic instability were applied. Sham-operated rats served as controis. Organ function was studied by monitoring standard serum parameters. The inflammatory status of the liver was assessed by determining the immediate early gene products, the expression of cell adhesion molecules, and the influx of leukocytes in the liver. Results: Progressive organ dysfunction was most pronounced in hemodynamically unstable brain-dead donors. Irrespective of hemodynamic status, a progressive inflammatory activation could be observed in brain-dead rats compared with controls. Conclusions: Brain death causes progressive liver dysfunction, which is made worse by the coexistence of hemodynamic instability. Further, brain death activates the inflammatory status of the potential donor liver, irrespective of the presence of hypotension. The changes observed may predispose the graft to additional damage from ischemia and reperfusion in the transplant procedure.",
author = "{Van Der Hoeven}, {Joost A.B.} and {Ter Horst}, {Gert J.} and Grietje Molema and {De Vos}, Paul and Girbes, {Armand R.J.} and Folkert Postema and Freund, {Reni L.} and Janneke Wiersema and {Van Schilfgaarde}, Reinout and Ploeg, {Rutger J.}",
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Van Der Hoeven, JAB, Ter Horst, GJ, Molema, G, De Vos, P, Girbes, ARJ, Postema, F, Freund, RL, Wiersema, J, Van Schilfgaarde, R & Ploeg, RJ 2000, 'Effects of brain death and hemodynamic status on function and immunologic activation of the potential donor liver in the rat' Annals of Surgery, vol. 232, no. 6, pp. 804-813. https://doi.org/10.1097/00000658-200012000-00009

Effects of brain death and hemodynamic status on function and immunologic activation of the potential donor liver in the rat. / Van Der Hoeven, Joost A.B.; Ter Horst, Gert J.; Molema, Grietje; De Vos, Paul; Girbes, Armand R.J.; Postema, Folkert; Freund, Reni L.; Wiersema, Janneke; Van Schilfgaarde, Reinout; Ploeg, Rutger J.

In: Annals of Surgery, Vol. 232, No. 6, 13.12.2000, p. 804-813.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Effects of brain death and hemodynamic status on function and immunologic activation of the potential donor liver in the rat

AU - Van Der Hoeven, Joost A.B.

AU - Ter Horst, Gert J.

AU - Molema, Grietje

AU - De Vos, Paul

AU - Girbes, Armand R.J.

AU - Postema, Folkert

AU - Freund, Reni L.

AU - Wiersema, Janneke

AU - Van Schilfgaarde, Reinout

AU - Ploeg, Rutger J.

PY - 2000/12/13

Y1 - 2000/12/13

N2 - Objective: To assess the effect on the function and immunologic status of potential donor livers of the duration of brain death combined with the presence and absence of hemodynamic instability in the donor. Summary Background Data: Brain death, regarded as a given condition in organ transplantation, could have significant effects on the donor organ quality. Methods: Brain death was induced in Wistar rats. Short or long periods of brain death in the presence or absence of hemodynamic instability were applied. Sham-operated rats served as controis. Organ function was studied by monitoring standard serum parameters. The inflammatory status of the liver was assessed by determining the immediate early gene products, the expression of cell adhesion molecules, and the influx of leukocytes in the liver. Results: Progressive organ dysfunction was most pronounced in hemodynamically unstable brain-dead donors. Irrespective of hemodynamic status, a progressive inflammatory activation could be observed in brain-dead rats compared with controls. Conclusions: Brain death causes progressive liver dysfunction, which is made worse by the coexistence of hemodynamic instability. Further, brain death activates the inflammatory status of the potential donor liver, irrespective of the presence of hypotension. The changes observed may predispose the graft to additional damage from ischemia and reperfusion in the transplant procedure.

AB - Objective: To assess the effect on the function and immunologic status of potential donor livers of the duration of brain death combined with the presence and absence of hemodynamic instability in the donor. Summary Background Data: Brain death, regarded as a given condition in organ transplantation, could have significant effects on the donor organ quality. Methods: Brain death was induced in Wistar rats. Short or long periods of brain death in the presence or absence of hemodynamic instability were applied. Sham-operated rats served as controis. Organ function was studied by monitoring standard serum parameters. The inflammatory status of the liver was assessed by determining the immediate early gene products, the expression of cell adhesion molecules, and the influx of leukocytes in the liver. Results: Progressive organ dysfunction was most pronounced in hemodynamically unstable brain-dead donors. Irrespective of hemodynamic status, a progressive inflammatory activation could be observed in brain-dead rats compared with controls. Conclusions: Brain death causes progressive liver dysfunction, which is made worse by the coexistence of hemodynamic instability. Further, brain death activates the inflammatory status of the potential donor liver, irrespective of the presence of hypotension. The changes observed may predispose the graft to additional damage from ischemia and reperfusion in the transplant procedure.

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DO - 10.1097/00000658-200012000-00009

M3 - Article

VL - 232

SP - 804

EP - 813

JO - Annals of Surgery

JF - Annals of Surgery

SN - 0003-4932

IS - 6

ER -