Abstract
Nitric oxide (NO) is essential for optimal myofilament function of the rat diaphragm in vitro during active shortening. Little is known about the role of NO in muscle contraction under hypoxic conditions. Hypoxia might increase the NO synthase (NOS) activity within the rat diaphragm. We hypothesized that NO plays a protective role in isotonic contractile and fatigue properties during hypoxia in vitro. The effects of the NOS inhibitor NG-monomethyl-L-arginine (L-NMMA), the NO scavenger hemoglobin, and the NO donor spermine NONOate on shortening velocity, power generation, and isotonic fatigability during hypoxia were evaluated (Po2 ∼ 7 kPa). L-NMMA and hemoglobin slowed the shortening velocity, depressed power generation, and increased isotonic fatigability during hypoxia. The effects of L-NMMA were prevented by coadministration with the NOS substrate L-arginine. Spermine NONOate did not alter isotonic contractile and fatigue properties during hypoxia. These results indicate that endogenous NO is needed for optimal muscle contraction of the rat diaphragm in vitro during hypoxia.
| Original language | English |
|---|---|
| Pages (from-to) | 612-620 |
| Number of pages | 9 |
| Journal | Journal of Applied Physiology |
| Volume | 94 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1 Feb 2003 |
Cite this
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Effects of modulation of nitric oxide on rat diaphragm isotonic contractility during hypoxia. / Zhu, Xiaoping; Heunks, Leo M.A.; Machiels, Herwin A.; Ennen, Leo; Dekhuijzen, P. N.Richard.
In: Journal of Applied Physiology, Vol. 94, No. 2, 01.02.2003, p. 612-620.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Effects of modulation of nitric oxide on rat diaphragm isotonic contractility during hypoxia
AU - Zhu, Xiaoping
AU - Heunks, Leo M.A.
AU - Machiels, Herwin A.
AU - Ennen, Leo
AU - Dekhuijzen, P. N.Richard
PY - 2003/2/1
Y1 - 2003/2/1
N2 - Nitric oxide (NO) is essential for optimal myofilament function of the rat diaphragm in vitro during active shortening. Little is known about the role of NO in muscle contraction under hypoxic conditions. Hypoxia might increase the NO synthase (NOS) activity within the rat diaphragm. We hypothesized that NO plays a protective role in isotonic contractile and fatigue properties during hypoxia in vitro. The effects of the NOS inhibitor NG-monomethyl-L-arginine (L-NMMA), the NO scavenger hemoglobin, and the NO donor spermine NONOate on shortening velocity, power generation, and isotonic fatigability during hypoxia were evaluated (Po2 ∼ 7 kPa). L-NMMA and hemoglobin slowed the shortening velocity, depressed power generation, and increased isotonic fatigability during hypoxia. The effects of L-NMMA were prevented by coadministration with the NOS substrate L-arginine. Spermine NONOate did not alter isotonic contractile and fatigue properties during hypoxia. These results indicate that endogenous NO is needed for optimal muscle contraction of the rat diaphragm in vitro during hypoxia.
AB - Nitric oxide (NO) is essential for optimal myofilament function of the rat diaphragm in vitro during active shortening. Little is known about the role of NO in muscle contraction under hypoxic conditions. Hypoxia might increase the NO synthase (NOS) activity within the rat diaphragm. We hypothesized that NO plays a protective role in isotonic contractile and fatigue properties during hypoxia in vitro. The effects of the NOS inhibitor NG-monomethyl-L-arginine (L-NMMA), the NO scavenger hemoglobin, and the NO donor spermine NONOate on shortening velocity, power generation, and isotonic fatigability during hypoxia were evaluated (Po2 ∼ 7 kPa). L-NMMA and hemoglobin slowed the shortening velocity, depressed power generation, and increased isotonic fatigability during hypoxia. The effects of L-NMMA were prevented by coadministration with the NOS substrate L-arginine. Spermine NONOate did not alter isotonic contractile and fatigue properties during hypoxia. These results indicate that endogenous NO is needed for optimal muscle contraction of the rat diaphragm in vitro during hypoxia.
KW - Hemoglobin
KW - N-monomethyl-L-arginine
KW - Spermine NONOate
UR - http://www.scopus.com/inward/record.url?scp=0037311022&partnerID=8YFLogxK
U2 - 10.1152/japplphysiol.00441.2002
DO - 10.1152/japplphysiol.00441.2002
M3 - Article
VL - 94
SP - 612
EP - 620
JO - Journal of Applied Physiology
JF - Journal of Applied Physiology
SN - 8750-7587
IS - 2
ER -