Enhancing osteoconduction of PLLA-based nanocomposite scaffolds for bone regeneration using different biomimetic signals to MSCs

Gabriela Ciapetti, Donatella Granchi, Valentina Devescovi, Serena R Baglio, Elisa Leonardi, Desirèe Martini, Maria Jesus Jurado, Beatriz Olalde, Ilaria Armentano, Josè M Kenny, Frank X Walboomers, Josè Inaki Alava, Nicola Baldini

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

In bone engineering, the adhesion, proliferation and differentiation of mesenchymal stromal cells rely on signaling from chemico-physical structure of the substrate, therefore prompting the design of mimetic "extracellular matrix"-like scaffolds. In this study, three-dimensional porous poly-L-lactic acid (PLLA)-based scaffolds have been mixed with different components, including single walled carbon nanotubes (CNT), micro-hydroxyapatite particles (HA), and BMP2, and treated with plasma (PT), to obtain four different nanocomposites: PLLA + CNT, PLLA + CNTHA, PLLA + CNT + HA + BMP2 and PLLA + CNT + HA + PT. Adult bone marrow mesenchymal stromal cells (MSCs) were derived from the femur of orthopaedic patients, seeded on the scaffolds and cultured under osteogenic induction up to differentiation and mineralization. The release of specific metabolites and temporal gene expression profiles of marrow-derived osteoprogenitors were analyzed at definite time points, relevant to in vitro culture as well as in vivo differentiation. As a result, the role of the different biomimetic components added to the PLLA matrix was deciphered, with BMP2-added scaffolds showing the highest biomimetic activity on cells differentiating to mature osteoblasts. The modification of a polymeric scaffold with reinforcing components which also work as biomimetic cues for cells can effectively direct osteoprogenitor cells differentiation, so as to shorten the time required for mineralization.

Original languageEnglish
Pages (from-to)2439-58
Number of pages20
JournalInternational Journal of Molecular Sciences
Volume13
Issue number2
DOIs
Publication statusPublished - 2012

Cite this

Ciapetti, Gabriela ; Granchi, Donatella ; Devescovi, Valentina ; Baglio, Serena R ; Leonardi, Elisa ; Martini, Desirèe ; Jurado, Maria Jesus ; Olalde, Beatriz ; Armentano, Ilaria ; Kenny, Josè M ; Walboomers, Frank X ; Alava, Josè Inaki ; Baldini, Nicola. / Enhancing osteoconduction of PLLA-based nanocomposite scaffolds for bone regeneration using different biomimetic signals to MSCs. In: International Journal of Molecular Sciences. 2012 ; Vol. 13, No. 2. pp. 2439-58.
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author = "Gabriela Ciapetti and Donatella Granchi and Valentina Devescovi and Baglio, {Serena R} and Elisa Leonardi and Desir{\`e}e Martini and Jurado, {Maria Jesus} and Beatriz Olalde and Ilaria Armentano and Kenny, {Jos{\`e} M} and Walboomers, {Frank X} and Alava, {Jos{\`e} Inaki} and Nicola Baldini",
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Ciapetti, G, Granchi, D, Devescovi, V, Baglio, SR, Leonardi, E, Martini, D, Jurado, MJ, Olalde, B, Armentano, I, Kenny, JM, Walboomers, FX, Alava, JI & Baldini, N 2012, 'Enhancing osteoconduction of PLLA-based nanocomposite scaffolds for bone regeneration using different biomimetic signals to MSCs' International Journal of Molecular Sciences, vol. 13, no. 2, pp. 2439-58. https://doi.org/10.3390/ijms13022439

Enhancing osteoconduction of PLLA-based nanocomposite scaffolds for bone regeneration using different biomimetic signals to MSCs. / Ciapetti, Gabriela; Granchi, Donatella; Devescovi, Valentina; Baglio, Serena R; Leonardi, Elisa; Martini, Desirèe; Jurado, Maria Jesus; Olalde, Beatriz; Armentano, Ilaria; Kenny, Josè M; Walboomers, Frank X; Alava, Josè Inaki; Baldini, Nicola.

In: International Journal of Molecular Sciences, Vol. 13, No. 2, 2012, p. 2439-58.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Enhancing osteoconduction of PLLA-based nanocomposite scaffolds for bone regeneration using different biomimetic signals to MSCs

AU - Ciapetti, Gabriela

AU - Granchi, Donatella

AU - Devescovi, Valentina

AU - Baglio, Serena R

AU - Leonardi, Elisa

AU - Martini, Desirèe

AU - Jurado, Maria Jesus

AU - Olalde, Beatriz

AU - Armentano, Ilaria

AU - Kenny, Josè M

AU - Walboomers, Frank X

AU - Alava, Josè Inaki

AU - Baldini, Nicola

PY - 2012

Y1 - 2012

N2 - In bone engineering, the adhesion, proliferation and differentiation of mesenchymal stromal cells rely on signaling from chemico-physical structure of the substrate, therefore prompting the design of mimetic "extracellular matrix"-like scaffolds. In this study, three-dimensional porous poly-L-lactic acid (PLLA)-based scaffolds have been mixed with different components, including single walled carbon nanotubes (CNT), micro-hydroxyapatite particles (HA), and BMP2, and treated with plasma (PT), to obtain four different nanocomposites: PLLA + CNT, PLLA + CNTHA, PLLA + CNT + HA + BMP2 and PLLA + CNT + HA + PT. Adult bone marrow mesenchymal stromal cells (MSCs) were derived from the femur of orthopaedic patients, seeded on the scaffolds and cultured under osteogenic induction up to differentiation and mineralization. The release of specific metabolites and temporal gene expression profiles of marrow-derived osteoprogenitors were analyzed at definite time points, relevant to in vitro culture as well as in vivo differentiation. As a result, the role of the different biomimetic components added to the PLLA matrix was deciphered, with BMP2-added scaffolds showing the highest biomimetic activity on cells differentiating to mature osteoblasts. The modification of a polymeric scaffold with reinforcing components which also work as biomimetic cues for cells can effectively direct osteoprogenitor cells differentiation, so as to shorten the time required for mineralization.

AB - In bone engineering, the adhesion, proliferation and differentiation of mesenchymal stromal cells rely on signaling from chemico-physical structure of the substrate, therefore prompting the design of mimetic "extracellular matrix"-like scaffolds. In this study, three-dimensional porous poly-L-lactic acid (PLLA)-based scaffolds have been mixed with different components, including single walled carbon nanotubes (CNT), micro-hydroxyapatite particles (HA), and BMP2, and treated with plasma (PT), to obtain four different nanocomposites: PLLA + CNT, PLLA + CNTHA, PLLA + CNT + HA + BMP2 and PLLA + CNT + HA + PT. Adult bone marrow mesenchymal stromal cells (MSCs) were derived from the femur of orthopaedic patients, seeded on the scaffolds and cultured under osteogenic induction up to differentiation and mineralization. The release of specific metabolites and temporal gene expression profiles of marrow-derived osteoprogenitors were analyzed at definite time points, relevant to in vitro culture as well as in vivo differentiation. As a result, the role of the different biomimetic components added to the PLLA matrix was deciphered, with BMP2-added scaffolds showing the highest biomimetic activity on cells differentiating to mature osteoblasts. The modification of a polymeric scaffold with reinforcing components which also work as biomimetic cues for cells can effectively direct osteoprogenitor cells differentiation, so as to shorten the time required for mineralization.

KW - Aged

KW - Biomimetic Materials

KW - Bone Regeneration

KW - Cell Culture Techniques

KW - Cell Differentiation

KW - Cell Proliferation

KW - Cells, Cultured

KW - Female

KW - Guided Tissue Regeneration

KW - Humans

KW - Lactic Acid

KW - Male

KW - Mesenchymal Stromal Cells

KW - Middle Aged

KW - Nanocomposites

KW - Osteoblasts

KW - Osteogenesis

KW - Polyesters

KW - Polymers

KW - Signal Transduction

KW - Tissue Engineering

KW - Tissue Scaffolds

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.3390/ijms13022439

DO - 10.3390/ijms13022439

M3 - Article

VL - 13

SP - 2439

EP - 2458

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1422-0067

IS - 2

ER -