Eosinophils capture viruses, a capacity that is defective in asthma

Yanaika S. Sabogal Piñeros, Suzanne M. Bal, Annemiek Dijkhuis, Christof J. Majoor, Barbara S. Dierdorp, Tamara Dekker, Esmée P. Hoefsmit, Peter I. Bonta, Daisy Picavet, Nicole N. van der Wel, Leo Koenderman, Peter J. Sterk, Lara Ravanetti, René Lutter

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Background: Activated eosinophils cause major pathology in stable and exacerbating asthma; however, they can also display protective properties like an extracellular antiviral activity. Initial murine studies led us to further explore a potential intracellular antiviral activity by eosinophils. Methods: To follow eosinophil-virus interaction, respiratory syncytial virus (RSV) and influenza virus were labeled with a fluorescent lipophilic dye (DiD). Interactions with eosinophils were visualized by confocal microscopy, electron microscopy, and flow cytometry. Eosinophil activation was assessed by both flow cytometry and ELISA. In a separate study, eosinophils were depleted in asthma patients using anti-IL-5 (mepolizumab), followed by a challenge with rhinovirus-16 (RV16). Results: DiD-RSV and DiD-influenza rapidly adhered to human eosinophils and were internalized and inactivated (95% in ≤ 2 hours) as reflected by a reduced replication in epithelial cells. The capacity of eosinophils to capture virus was reduced up to 75% with increasing severity of asthma. Eosinophils were activated by virus in vitro and in vivo. In vivo this correlated with virus-induced loss of asthma control. Conclusions: This previously unrecognized and in asthma attenuated antiviral property provides a new perspective to eosinophils in asthma. This is indicative of an imbalance between protective and cytotoxic properties by eosinophils that may underlie asthma exacerbations.
Original languageEnglish
JournalAllergy: European Journal of Allergy and Clinical Immunology
Volume74
DOIs
Publication statusPublished - 1 Oct 2019
Externally publishedYes

Cite this

Sabogal Piñeros, Yanaika S. ; Bal, Suzanne M. ; Dijkhuis, Annemiek ; Majoor, Christof J. ; Dierdorp, Barbara S. ; Dekker, Tamara ; Hoefsmit, Esmée P. ; Bonta, Peter I. ; Picavet, Daisy ; van der Wel, Nicole N. ; Koenderman, Leo ; Sterk, Peter J. ; Ravanetti, Lara ; Lutter, René. / Eosinophils capture viruses, a capacity that is defective in asthma. In: Allergy: European Journal of Allergy and Clinical Immunology. 2019 ; Vol. 74.
@article{4d4ea08ad28f475b8abf9b23e1f7465a,
title = "Eosinophils capture viruses, a capacity that is defective in asthma",
abstract = "Background: Activated eosinophils cause major pathology in stable and exacerbating asthma; however, they can also display protective properties like an extracellular antiviral activity. Initial murine studies led us to further explore a potential intracellular antiviral activity by eosinophils. Methods: To follow eosinophil-virus interaction, respiratory syncytial virus (RSV) and influenza virus were labeled with a fluorescent lipophilic dye (DiD). Interactions with eosinophils were visualized by confocal microscopy, electron microscopy, and flow cytometry. Eosinophil activation was assessed by both flow cytometry and ELISA. In a separate study, eosinophils were depleted in asthma patients using anti-IL-5 (mepolizumab), followed by a challenge with rhinovirus-16 (RV16). Results: DiD-RSV and DiD-influenza rapidly adhered to human eosinophils and were internalized and inactivated (95{\%} in ≤ 2 hours) as reflected by a reduced replication in epithelial cells. The capacity of eosinophils to capture virus was reduced up to 75{\%} with increasing severity of asthma. Eosinophils were activated by virus in vitro and in vivo. In vivo this correlated with virus-induced loss of asthma control. Conclusions: This previously unrecognized and in asthma attenuated antiviral property provides a new perspective to eosinophils in asthma. This is indicative of an imbalance between protective and cytotoxic properties by eosinophils that may underlie asthma exacerbations.",
author = "{Sabogal Pi{\~n}eros}, {Yanaika S.} and Bal, {Suzanne M.} and Annemiek Dijkhuis and Majoor, {Christof J.} and Dierdorp, {Barbara S.} and Tamara Dekker and Hoefsmit, {Esm{\'e}e P.} and Bonta, {Peter I.} and Daisy Picavet and {van der Wel}, {Nicole N.} and Leo Koenderman and Sterk, {Peter J.} and Lara Ravanetti and Ren{\'e} Lutter",
year = "2019",
month = "10",
day = "1",
doi = "10.1111/all.13802",
language = "English",
volume = "74",
journal = "Allergy",
issn = "0105-4538",
publisher = "Wiley-Blackwell",

}

Sabogal Piñeros, YS, Bal, SM, Dijkhuis, A, Majoor, CJ, Dierdorp, BS, Dekker, T, Hoefsmit, EP, Bonta, PI, Picavet, D, van der Wel, NN, Koenderman, L, Sterk, PJ, Ravanetti, L & Lutter, R 2019, 'Eosinophils capture viruses, a capacity that is defective in asthma' Allergy: European Journal of Allergy and Clinical Immunology, vol. 74. https://doi.org/10.1111/all.13802

Eosinophils capture viruses, a capacity that is defective in asthma. / Sabogal Piñeros, Yanaika S.; Bal, Suzanne M.; Dijkhuis, Annemiek; Majoor, Christof J.; Dierdorp, Barbara S.; Dekker, Tamara; Hoefsmit, Esmée P.; Bonta, Peter I.; Picavet, Daisy; van der Wel, Nicole N.; Koenderman, Leo; Sterk, Peter J.; Ravanetti, Lara; Lutter, René.

In: Allergy: European Journal of Allergy and Clinical Immunology, Vol. 74, 01.10.2019.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Eosinophils capture viruses, a capacity that is defective in asthma

AU - Sabogal Piñeros, Yanaika S.

AU - Bal, Suzanne M.

AU - Dijkhuis, Annemiek

AU - Majoor, Christof J.

AU - Dierdorp, Barbara S.

AU - Dekker, Tamara

AU - Hoefsmit, Esmée P.

AU - Bonta, Peter I.

AU - Picavet, Daisy

AU - van der Wel, Nicole N.

AU - Koenderman, Leo

AU - Sterk, Peter J.

AU - Ravanetti, Lara

AU - Lutter, René

PY - 2019/10/1

Y1 - 2019/10/1

N2 - Background: Activated eosinophils cause major pathology in stable and exacerbating asthma; however, they can also display protective properties like an extracellular antiviral activity. Initial murine studies led us to further explore a potential intracellular antiviral activity by eosinophils. Methods: To follow eosinophil-virus interaction, respiratory syncytial virus (RSV) and influenza virus were labeled with a fluorescent lipophilic dye (DiD). Interactions with eosinophils were visualized by confocal microscopy, electron microscopy, and flow cytometry. Eosinophil activation was assessed by both flow cytometry and ELISA. In a separate study, eosinophils were depleted in asthma patients using anti-IL-5 (mepolizumab), followed by a challenge with rhinovirus-16 (RV16). Results: DiD-RSV and DiD-influenza rapidly adhered to human eosinophils and were internalized and inactivated (95% in ≤ 2 hours) as reflected by a reduced replication in epithelial cells. The capacity of eosinophils to capture virus was reduced up to 75% with increasing severity of asthma. Eosinophils were activated by virus in vitro and in vivo. In vivo this correlated with virus-induced loss of asthma control. Conclusions: This previously unrecognized and in asthma attenuated antiviral property provides a new perspective to eosinophils in asthma. This is indicative of an imbalance between protective and cytotoxic properties by eosinophils that may underlie asthma exacerbations.

AB - Background: Activated eosinophils cause major pathology in stable and exacerbating asthma; however, they can also display protective properties like an extracellular antiviral activity. Initial murine studies led us to further explore a potential intracellular antiviral activity by eosinophils. Methods: To follow eosinophil-virus interaction, respiratory syncytial virus (RSV) and influenza virus were labeled with a fluorescent lipophilic dye (DiD). Interactions with eosinophils were visualized by confocal microscopy, electron microscopy, and flow cytometry. Eosinophil activation was assessed by both flow cytometry and ELISA. In a separate study, eosinophils were depleted in asthma patients using anti-IL-5 (mepolizumab), followed by a challenge with rhinovirus-16 (RV16). Results: DiD-RSV and DiD-influenza rapidly adhered to human eosinophils and were internalized and inactivated (95% in ≤ 2 hours) as reflected by a reduced replication in epithelial cells. The capacity of eosinophils to capture virus was reduced up to 75% with increasing severity of asthma. Eosinophils were activated by virus in vitro and in vivo. In vivo this correlated with virus-induced loss of asthma control. Conclusions: This previously unrecognized and in asthma attenuated antiviral property provides a new perspective to eosinophils in asthma. This is indicative of an imbalance between protective and cytotoxic properties by eosinophils that may underlie asthma exacerbations.

UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85065962069&origin=inward

UR - https://www.ncbi.nlm.nih.gov/pubmed/30934128

U2 - 10.1111/all.13802

DO - 10.1111/all.13802

M3 - Article

VL - 74

JO - Allergy

JF - Allergy

SN - 0105-4538

ER -