TY - JOUR
T1 - Ets proteins
T2 - New factors that regulate immunoglobulin heavy-chain gene expression
AU - Rivera, Richard R.
AU - Stuiver, Maarten H.
AU - Steenbergen, Renske
AU - Murre, Cornelis
PY - 1993/11
Y1 - 1993/11
N2 - We used a DNA-protein interaction screening method to isolate a cDNA, Erg-3, whose product binds to a site, designated π, present in the immunoglobulin (Ig) heavy-chain gene enhancer. Erg-3 is an alternatively spliced product of the erg gene and contains an Ets DNA-binding domain. Fli-1 and PU.1, related Ets proteins, also bind to the same site. In addition, PU.1 binds to a second site, designated μB, in the Ig heavy-chain enhancer. We demonstrate that the π binding site is crucial for Ig heavy-chain gene enhancer function. In addition, we show that Erg-3 and Fli.1, but not PU.1, can activate a reporter construct containing a multimer of protein-binding sites, synergistically with helix-loop-helix protein E12. We discuss how combinatorial interactions between members of the helix-loop-helix and Ets families may account for the tissue specificity of these proteins.
AB - We used a DNA-protein interaction screening method to isolate a cDNA, Erg-3, whose product binds to a site, designated π, present in the immunoglobulin (Ig) heavy-chain gene enhancer. Erg-3 is an alternatively spliced product of the erg gene and contains an Ets DNA-binding domain. Fli-1 and PU.1, related Ets proteins, also bind to the same site. In addition, PU.1 binds to a second site, designated μB, in the Ig heavy-chain enhancer. We demonstrate that the π binding site is crucial for Ig heavy-chain gene enhancer function. In addition, we show that Erg-3 and Fli.1, but not PU.1, can activate a reporter construct containing a multimer of protein-binding sites, synergistically with helix-loop-helix protein E12. We discuss how combinatorial interactions between members of the helix-loop-helix and Ets families may account for the tissue specificity of these proteins.
UR - http://www.scopus.com/inward/record.url?scp=0027382099&partnerID=8YFLogxK
U2 - 10.1128/MCB.13.11.7163
DO - 10.1128/MCB.13.11.7163
M3 - Article
C2 - 8413305
AN - SCOPUS:0027382099
VL - 13
SP - 7163
EP - 7169
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
SN - 0270-7306
IS - 11
ER -