Ets proteins: New factors that regulate immunoglobulin heavy-chain gene expression

Richard R. Rivera, Maarten H. Stuiver, Renske Steenbergen, Cornelis Murre*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

We used a DNA-protein interaction screening method to isolate a cDNA, Erg-3, whose product binds to a site, designated π, present in the immunoglobulin (Ig) heavy-chain gene enhancer. Erg-3 is an alternatively spliced product of the erg gene and contains an Ets DNA-binding domain. Fli-1 and PU.1, related Ets proteins, also bind to the same site. In addition, PU.1 binds to a second site, designated μB, in the Ig heavy-chain enhancer. We demonstrate that the π binding site is crucial for Ig heavy-chain gene enhancer function. In addition, we show that Erg-3 and Fli.1, but not PU.1, can activate a reporter construct containing a multimer of protein-binding sites, synergistically with helix-loop-helix protein E12. We discuss how combinatorial interactions between members of the helix-loop-helix and Ets families may account for the tissue specificity of these proteins.

Original languageEnglish
Pages (from-to)7163-7169
Number of pages7
JournalMolecular and Cellular Biology
Volume13
Issue number11
DOIs
Publication statusPublished - Nov 1993
Externally publishedYes

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