Expression and analysis of the Epstein-Barr virus BARF1-encoded protein from a tetracycline-regulatable adenovirus system

M de Turenne-Tessier, P Jolicoeur, J M Middeldorp, T Ooka

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Epstein-Barr virus (EBV) has been associated with human cancers of lymphocytic or epithelial origin. Potential functions of the BARF1 early gene in EBV oncogenesis emerged from our observations showing expression of BARF1-encoded protein in nasopharyngeal carcinoma biopsies, and induction of either malignant transformation (in rodent fibroblast and human B cell lines) or immortalization (in monkey primary epithelial cells) following BARF1 transfection. We previously reported expression of the BARF1 product as a cytoplasm/membrane-associated protein from 293-tTA cells infected with a BARF1-recombinant adenovirus. Since constitutive expression of BARF1 from this heterologous system became inefficient, we developed a tetracycline-regulatable recombinant vector expressing BARF1 and green fluorescent protein from a dicistronic message. As here reported, stable and efficient expression of BARF1 from this vector in either permissive or non-permissive cell lines, allowed the first sequencing identification and further molecular characterization of BARF1-encoded protein.

Original languageEnglish
Pages (from-to)9-18
Number of pages10
JournalVirus evolution
Volume109
Issue number1
DOIs
Publication statusPublished - Apr 2005

Cite this

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title = "Expression and analysis of the Epstein-Barr virus BARF1-encoded protein from a tetracycline-regulatable adenovirus system",
abstract = "Epstein-Barr virus (EBV) has been associated with human cancers of lymphocytic or epithelial origin. Potential functions of the BARF1 early gene in EBV oncogenesis emerged from our observations showing expression of BARF1-encoded protein in nasopharyngeal carcinoma biopsies, and induction of either malignant transformation (in rodent fibroblast and human B cell lines) or immortalization (in monkey primary epithelial cells) following BARF1 transfection. We previously reported expression of the BARF1 product as a cytoplasm/membrane-associated protein from 293-tTA cells infected with a BARF1-recombinant adenovirus. Since constitutive expression of BARF1 from this heterologous system became inefficient, we developed a tetracycline-regulatable recombinant vector expressing BARF1 and green fluorescent protein from a dicistronic message. As here reported, stable and efficient expression of BARF1 from this vector in either permissive or non-permissive cell lines, allowed the first sequencing identification and further molecular characterization of BARF1-encoded protein.",
keywords = "Adenoviridae, Gene Expression Regulation, Viral, Genetic Vectors, Green Fluorescent Proteins, Herpesvirus 4, Human, Humans, Molecular Weight, Recombinant Proteins, Tetracycline, Viral Proteins, Journal Article, Research Support, Non-U.S. Gov't",
author = "{de Turenne-Tessier}, M and P Jolicoeur and Middeldorp, {J M} and T Ooka",
year = "2005",
month = "4",
doi = "10.1016/j.virusres.2004.10.003",
language = "English",
volume = "109",
pages = "9--18",
journal = "Virus evolution",
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Expression and analysis of the Epstein-Barr virus BARF1-encoded protein from a tetracycline-regulatable adenovirus system. / de Turenne-Tessier, M; Jolicoeur, P; Middeldorp, J M; Ooka, T.

In: Virus evolution, Vol. 109, No. 1, 04.2005, p. 9-18.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - de Turenne-Tessier, M

AU - Jolicoeur, P

AU - Middeldorp, J M

AU - Ooka, T

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AB - Epstein-Barr virus (EBV) has been associated with human cancers of lymphocytic or epithelial origin. Potential functions of the BARF1 early gene in EBV oncogenesis emerged from our observations showing expression of BARF1-encoded protein in nasopharyngeal carcinoma biopsies, and induction of either malignant transformation (in rodent fibroblast and human B cell lines) or immortalization (in monkey primary epithelial cells) following BARF1 transfection. We previously reported expression of the BARF1 product as a cytoplasm/membrane-associated protein from 293-tTA cells infected with a BARF1-recombinant adenovirus. Since constitutive expression of BARF1 from this heterologous system became inefficient, we developed a tetracycline-regulatable recombinant vector expressing BARF1 and green fluorescent protein from a dicistronic message. As here reported, stable and efficient expression of BARF1 from this vector in either permissive or non-permissive cell lines, allowed the first sequencing identification and further molecular characterization of BARF1-encoded protein.

KW - Adenoviridae

KW - Gene Expression Regulation, Viral

KW - Genetic Vectors

KW - Green Fluorescent Proteins

KW - Herpesvirus 4, Human

KW - Humans

KW - Molecular Weight

KW - Recombinant Proteins

KW - Tetracycline

KW - Viral Proteins

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

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SN - 2057-1577

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