Expression of epstein-barr virus encoded nuclear antigen 1 in benign and malignant tissues harbouring EBV

J J Oudejans, D F Dukers, N M Jiwa, A J van den Brule, F A Grässer, P C de Bruin, A Horstman, W Vos, J van Gorp, J M Middeldorp, C J Meijer

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

AIMS: To determine levels of expression of Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) in benign and malignant tissues harbouring EBV in relation to EBNA1 promoter usage.

METHODS: Expression of EBNA1 was investigated by means of immunohistochemistry using a mixture of two EBNA1 specific monoclonal antibodies, 1H4-1 and 2B4-1. The presence of EBV was detected by EBER1/2 RNA in situ hybridisation. Detection of promoter specific EBNA1 transcripts was by RT-PCR analysis.

RESULTS: EBNA1 positive cells were detected in all 20 EBV associated B cell lymphomas, 18 of which had arisen in immunocompromised patients; in eight of nine EBV associated T cell lymphomas; in 11 of 27 EBV positive cases of Hodgkin's disease; and in reactive lymphoid tissue harbouring EBV, including four cases of infectious mononucleosis. A diffuse EBNA1 staining pattern was observed in most of the EBV associated B cell lymphomas and was comparable with the EBER1/2 staining pattern. In the T cell lymphomas the number of EBNA1 positive cells was usually considerably less than the number of EBER1/2 positive ones. RT-PCR analysis revealed that in tumours with restricted EBNA1 expression-that is, T cell lymphomas and Hodgkin's disease lesions, EBNA1 transcripts were usually generated only by the F/Q promoter, whereas in B cell lymphomas EBNA1 transcripts were usually generated by both the C/W and F/Q promoters.

CONCLUSIONS: EBNA1 is expressed in all types of tissue harbouring EBV, but the level of expression varies greatly. This may be the result of differential promoter usage.

Original languageEnglish
Pages (from-to)897-902
Number of pages6
JournalJournal of Clinical Pathology
Volume49
Issue number11
Publication statusPublished - Nov 1996

Cite this

Oudejans, J. J., Dukers, D. F., Jiwa, N. M., van den Brule, A. J., Grässer, F. A., de Bruin, P. C., ... Meijer, C. J. (1996). Expression of epstein-barr virus encoded nuclear antigen 1 in benign and malignant tissues harbouring EBV. Journal of Clinical Pathology, 49(11), 897-902.