Expression of Epstein-Barr-virus-related nuclear antigens and B-cell markers in lymphomas of SIV-immunosuppressed monkeys

S L Li, H Feichtinger, E Kaaya, P Migliorini, P Putkonen, G Biberfeld, J M Middeldorp, P Biberfeld, I Ernberg

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Simian-immunodeficiency-virus(SIV)-infected cynomolgus monkeys develop B-cell lymphomas in approximately one third of the cases. We have now studied the expression of cynomolgus-Epstein-Barr-virus(cyno-EBV) nuclear antigens in 13 cyno-EBV-carrying SIVsm-associated monkey lymphomas and established cell lines from 3 of these tumors. Immunoblots of cell lysates were probed with polyspecific and monospecific reagents directed against human EB-virus EBNAI-6, and against the membrane protein LMPI. An EBNA2-cross-reacting protein was demonstrated in 8 lymphoma tissues (8/13) and in the 3 cell lines derived from the tumors. All tumors expressed a polypeptide with 50 to 55 kDa molecular weight, which cross-reacted with some antibodies to EBNAI. Absorption experiments with normal monkey tissue showed that this polypeptide was specific for the cyno-EBV-carrying lymphoma cells. Equivalents of EBNA3-6 and LMPI could not be detected. Immunophenotypical characterization showed that the monkey lymphomas were similar to human HIV-associated B-cell lymphomas. Malignant B-cell lymphomas in experimentally SIVsm-infected cynomolgus monkeys can be a model for EBV-associated lymphomagenesis in immunodeficiency states.

Original languageEnglish
Pages (from-to)609-15
Number of pages7
JournalInternational Journal of Cancer
Volume55
Issue number4
Publication statusPublished - 21 Oct 1993

Cite this

Li, S. L., Feichtinger, H., Kaaya, E., Migliorini, P., Putkonen, P., Biberfeld, G., ... Ernberg, I. (1993). Expression of Epstein-Barr-virus-related nuclear antigens and B-cell markers in lymphomas of SIV-immunosuppressed monkeys. International Journal of Cancer, 55(4), 609-15.
Li, S L ; Feichtinger, H ; Kaaya, E ; Migliorini, P ; Putkonen, P ; Biberfeld, G ; Middeldorp, J M ; Biberfeld, P ; Ernberg, I. / Expression of Epstein-Barr-virus-related nuclear antigens and B-cell markers in lymphomas of SIV-immunosuppressed monkeys. In: International Journal of Cancer. 1993 ; Vol. 55, No. 4. pp. 609-15.
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abstract = "Simian-immunodeficiency-virus(SIV)-infected cynomolgus monkeys develop B-cell lymphomas in approximately one third of the cases. We have now studied the expression of cynomolgus-Epstein-Barr-virus(cyno-EBV) nuclear antigens in 13 cyno-EBV-carrying SIVsm-associated monkey lymphomas and established cell lines from 3 of these tumors. Immunoblots of cell lysates were probed with polyspecific and monospecific reagents directed against human EB-virus EBNAI-6, and against the membrane protein LMPI. An EBNA2-cross-reacting protein was demonstrated in 8 lymphoma tissues (8/13) and in the 3 cell lines derived from the tumors. All tumors expressed a polypeptide with 50 to 55 kDa molecular weight, which cross-reacted with some antibodies to EBNAI. Absorption experiments with normal monkey tissue showed that this polypeptide was specific for the cyno-EBV-carrying lymphoma cells. Equivalents of EBNA3-6 and LMPI could not be detected. Immunophenotypical characterization showed that the monkey lymphomas were similar to human HIV-associated B-cell lymphomas. Malignant B-cell lymphomas in experimentally SIVsm-infected cynomolgus monkeys can be a model for EBV-associated lymphomagenesis in immunodeficiency states.",
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Li, SL, Feichtinger, H, Kaaya, E, Migliorini, P, Putkonen, P, Biberfeld, G, Middeldorp, JM, Biberfeld, P & Ernberg, I 1993, 'Expression of Epstein-Barr-virus-related nuclear antigens and B-cell markers in lymphomas of SIV-immunosuppressed monkeys' International Journal of Cancer, vol. 55, no. 4, pp. 609-15.

Expression of Epstein-Barr-virus-related nuclear antigens and B-cell markers in lymphomas of SIV-immunosuppressed monkeys. / Li, S L; Feichtinger, H; Kaaya, E; Migliorini, P; Putkonen, P; Biberfeld, G; Middeldorp, J M; Biberfeld, P; Ernberg, I.

In: International Journal of Cancer, Vol. 55, No. 4, 21.10.1993, p. 609-15.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Expression of Epstein-Barr-virus-related nuclear antigens and B-cell markers in lymphomas of SIV-immunosuppressed monkeys

AU - Li, S L

AU - Feichtinger, H

AU - Kaaya, E

AU - Migliorini, P

AU - Putkonen, P

AU - Biberfeld, G

AU - Middeldorp, J M

AU - Biberfeld, P

AU - Ernberg, I

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N2 - Simian-immunodeficiency-virus(SIV)-infected cynomolgus monkeys develop B-cell lymphomas in approximately one third of the cases. We have now studied the expression of cynomolgus-Epstein-Barr-virus(cyno-EBV) nuclear antigens in 13 cyno-EBV-carrying SIVsm-associated monkey lymphomas and established cell lines from 3 of these tumors. Immunoblots of cell lysates were probed with polyspecific and monospecific reagents directed against human EB-virus EBNAI-6, and against the membrane protein LMPI. An EBNA2-cross-reacting protein was demonstrated in 8 lymphoma tissues (8/13) and in the 3 cell lines derived from the tumors. All tumors expressed a polypeptide with 50 to 55 kDa molecular weight, which cross-reacted with some antibodies to EBNAI. Absorption experiments with normal monkey tissue showed that this polypeptide was specific for the cyno-EBV-carrying lymphoma cells. Equivalents of EBNA3-6 and LMPI could not be detected. Immunophenotypical characterization showed that the monkey lymphomas were similar to human HIV-associated B-cell lymphomas. Malignant B-cell lymphomas in experimentally SIVsm-infected cynomolgus monkeys can be a model for EBV-associated lymphomagenesis in immunodeficiency states.

AB - Simian-immunodeficiency-virus(SIV)-infected cynomolgus monkeys develop B-cell lymphomas in approximately one third of the cases. We have now studied the expression of cynomolgus-Epstein-Barr-virus(cyno-EBV) nuclear antigens in 13 cyno-EBV-carrying SIVsm-associated monkey lymphomas and established cell lines from 3 of these tumors. Immunoblots of cell lysates were probed with polyspecific and monospecific reagents directed against human EB-virus EBNAI-6, and against the membrane protein LMPI. An EBNA2-cross-reacting protein was demonstrated in 8 lymphoma tissues (8/13) and in the 3 cell lines derived from the tumors. All tumors expressed a polypeptide with 50 to 55 kDa molecular weight, which cross-reacted with some antibodies to EBNAI. Absorption experiments with normal monkey tissue showed that this polypeptide was specific for the cyno-EBV-carrying lymphoma cells. Equivalents of EBNA3-6 and LMPI could not be detected. Immunophenotypical characterization showed that the monkey lymphomas were similar to human HIV-associated B-cell lymphomas. Malignant B-cell lymphomas in experimentally SIVsm-infected cynomolgus monkeys can be a model for EBV-associated lymphomagenesis in immunodeficiency states.

KW - Animals

KW - Antigens, CD

KW - Antigens, Nuclear

KW - Antigens, Viral

KW - Blotting, Western

KW - Cross Reactions

KW - DNA-Binding Proteins

KW - Epstein-Barr Virus Nuclear Antigens

KW - Herpesvirus 4, Human

KW - Immunohistochemistry

KW - Lymphoma, B-Cell

KW - Macaca fascicularis

KW - Nuclear Proteins

KW - Simian Immunodeficiency Virus

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Article

VL - 55

SP - 609

EP - 615

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 4

ER -

Li SL, Feichtinger H, Kaaya E, Migliorini P, Putkonen P, Biberfeld G et al. Expression of Epstein-Barr-virus-related nuclear antigens and B-cell markers in lymphomas of SIV-immunosuppressed monkeys. International Journal of Cancer. 1993 Oct 21;55(4):609-15.