Expression of interleukin-1 beta in rat dorsal root ganglia

J C Copray, I Mantingh, N Brouwer, K Biber, B M Küst, R S Liem, I Huitinga, F J Tilders, A M Van Dam, H W Boddeke

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The expression of interleukin-1beta was examined in dorsal root ganglion (DRG) neurons from adult rats using non-radioactive in situ hybridization and immunocytochemistry. At all spinal levels, approximately 70% of the DRG neurons appeared to express IL-1beta mRNA; about 80% of these DRG neurons actually appeared to produce the IL-1beta protein at markedly varying levels. The expression of IL-1beta was found in large as well as in intermediate diameter sensory neurons but only sporadically in the population of small sensory neurons. The population of IL-1beta immunopositive sensory neurons included most of the large calretinin-positive Ia afferents, but only a few of the small substance P/CGRP positive sensory neurons. In situ hybridization staining for the detection of type 1 IL-1 receptor showed expression of this receptor by most of the sensory neurons as well as by supportive glial-like cells, presumably satellite cells. The functional significance of IL-1beta in the DRG neurons needs to be elucidated, but we speculate that IL-1beta produced by DRG neurons may be an auto/paracrine signalling molecule in sensory transmission.

Original languageEnglish
Pages (from-to)203-11
Number of pages9
JournalJournal of Neuroimmunology
Volume118
Issue number2
Publication statusPublished - 30 Aug 2001

Cite this

Copray, J. C., Mantingh, I., Brouwer, N., Biber, K., Küst, B. M., Liem, R. S., ... Boddeke, H. W. (2001). Expression of interleukin-1 beta in rat dorsal root ganglia. Journal of Neuroimmunology, 118(2), 203-11.