Expression of interleukin-1 beta in rat dorsal root ganglia

J C Copray, I Mantingh, N Brouwer, K Biber, B M Küst, R S Liem, I Huitinga, F J Tilders, A M Van Dam, H W Boddeke

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The expression of interleukin-1beta was examined in dorsal root ganglion (DRG) neurons from adult rats using non-radioactive in situ hybridization and immunocytochemistry. At all spinal levels, approximately 70% of the DRG neurons appeared to express IL-1beta mRNA; about 80% of these DRG neurons actually appeared to produce the IL-1beta protein at markedly varying levels. The expression of IL-1beta was found in large as well as in intermediate diameter sensory neurons but only sporadically in the population of small sensory neurons. The population of IL-1beta immunopositive sensory neurons included most of the large calretinin-positive Ia afferents, but only a few of the small substance P/CGRP positive sensory neurons. In situ hybridization staining for the detection of type 1 IL-1 receptor showed expression of this receptor by most of the sensory neurons as well as by supportive glial-like cells, presumably satellite cells. The functional significance of IL-1beta in the DRG neurons needs to be elucidated, but we speculate that IL-1beta produced by DRG neurons may be an auto/paracrine signalling molecule in sensory transmission.

Original languageEnglish
Pages (from-to)203-11
Number of pages9
JournalJournal of Neuroimmunology
Volume118
Issue number2
Publication statusPublished - 30 Aug 2001

Cite this

Copray, J. C., Mantingh, I., Brouwer, N., Biber, K., Küst, B. M., Liem, R. S., ... Boddeke, H. W. (2001). Expression of interleukin-1 beta in rat dorsal root ganglia. Journal of Neuroimmunology, 118(2), 203-11.
Copray, J C ; Mantingh, I ; Brouwer, N ; Biber, K ; Küst, B M ; Liem, R S ; Huitinga, I ; Tilders, F J ; Van Dam, A M ; Boddeke, H W. / Expression of interleukin-1 beta in rat dorsal root ganglia. In: Journal of Neuroimmunology. 2001 ; Vol. 118, No. 2. pp. 203-11.
@article{4d7d2599b98e4f5ab4db6d645f8c769a,
title = "Expression of interleukin-1 beta in rat dorsal root ganglia",
abstract = "The expression of interleukin-1beta was examined in dorsal root ganglion (DRG) neurons from adult rats using non-radioactive in situ hybridization and immunocytochemistry. At all spinal levels, approximately 70{\%} of the DRG neurons appeared to express IL-1beta mRNA; about 80{\%} of these DRG neurons actually appeared to produce the IL-1beta protein at markedly varying levels. The expression of IL-1beta was found in large as well as in intermediate diameter sensory neurons but only sporadically in the population of small sensory neurons. The population of IL-1beta immunopositive sensory neurons included most of the large calretinin-positive Ia afferents, but only a few of the small substance P/CGRP positive sensory neurons. In situ hybridization staining for the detection of type 1 IL-1 receptor showed expression of this receptor by most of the sensory neurons as well as by supportive glial-like cells, presumably satellite cells. The functional significance of IL-1beta in the DRG neurons needs to be elucidated, but we speculate that IL-1beta produced by DRG neurons may be an auto/paracrine signalling molecule in sensory transmission.",
keywords = "Animals, Calbindin 2, Calcitonin Gene-Related Peptide, Cell Size, Cytoplasm, Female, Ganglia, Spinal, Immunohistochemistry, In Situ Hybridization, Interleukin-1, Male, Microscopy, Immunoelectron, Neuroglia, Neurons, Afferent, Polyribosomes, RNA, Messenger, Rats, Receptors, Interleukin-1, Receptors, Interleukin-1 Type I, S100 Calcium Binding Protein G, Substance P, Journal Article",
author = "Copray, {J C} and I Mantingh and N Brouwer and K Biber and K{\"u}st, {B M} and Liem, {R S} and I Huitinga and Tilders, {F J} and {Van Dam}, {A M} and Boddeke, {H W}",
year = "2001",
month = "8",
day = "30",
language = "English",
volume = "118",
pages = "203--11",
journal = "Journal of Neuroimmunology",
issn = "0165-5728",
publisher = "Elsevier",
number = "2",

}

Copray, JC, Mantingh, I, Brouwer, N, Biber, K, Küst, BM, Liem, RS, Huitinga, I, Tilders, FJ, Van Dam, AM & Boddeke, HW 2001, 'Expression of interleukin-1 beta in rat dorsal root ganglia' Journal of Neuroimmunology, vol. 118, no. 2, pp. 203-11.

Expression of interleukin-1 beta in rat dorsal root ganglia. / Copray, J C; Mantingh, I; Brouwer, N; Biber, K; Küst, B M; Liem, R S; Huitinga, I; Tilders, F J; Van Dam, A M; Boddeke, H W.

In: Journal of Neuroimmunology, Vol. 118, No. 2, 30.08.2001, p. 203-11.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Expression of interleukin-1 beta in rat dorsal root ganglia

AU - Copray, J C

AU - Mantingh, I

AU - Brouwer, N

AU - Biber, K

AU - Küst, B M

AU - Liem, R S

AU - Huitinga, I

AU - Tilders, F J

AU - Van Dam, A M

AU - Boddeke, H W

PY - 2001/8/30

Y1 - 2001/8/30

N2 - The expression of interleukin-1beta was examined in dorsal root ganglion (DRG) neurons from adult rats using non-radioactive in situ hybridization and immunocytochemistry. At all spinal levels, approximately 70% of the DRG neurons appeared to express IL-1beta mRNA; about 80% of these DRG neurons actually appeared to produce the IL-1beta protein at markedly varying levels. The expression of IL-1beta was found in large as well as in intermediate diameter sensory neurons but only sporadically in the population of small sensory neurons. The population of IL-1beta immunopositive sensory neurons included most of the large calretinin-positive Ia afferents, but only a few of the small substance P/CGRP positive sensory neurons. In situ hybridization staining for the detection of type 1 IL-1 receptor showed expression of this receptor by most of the sensory neurons as well as by supportive glial-like cells, presumably satellite cells. The functional significance of IL-1beta in the DRG neurons needs to be elucidated, but we speculate that IL-1beta produced by DRG neurons may be an auto/paracrine signalling molecule in sensory transmission.

AB - The expression of interleukin-1beta was examined in dorsal root ganglion (DRG) neurons from adult rats using non-radioactive in situ hybridization and immunocytochemistry. At all spinal levels, approximately 70% of the DRG neurons appeared to express IL-1beta mRNA; about 80% of these DRG neurons actually appeared to produce the IL-1beta protein at markedly varying levels. The expression of IL-1beta was found in large as well as in intermediate diameter sensory neurons but only sporadically in the population of small sensory neurons. The population of IL-1beta immunopositive sensory neurons included most of the large calretinin-positive Ia afferents, but only a few of the small substance P/CGRP positive sensory neurons. In situ hybridization staining for the detection of type 1 IL-1 receptor showed expression of this receptor by most of the sensory neurons as well as by supportive glial-like cells, presumably satellite cells. The functional significance of IL-1beta in the DRG neurons needs to be elucidated, but we speculate that IL-1beta produced by DRG neurons may be an auto/paracrine signalling molecule in sensory transmission.

KW - Animals

KW - Calbindin 2

KW - Calcitonin Gene-Related Peptide

KW - Cell Size

KW - Cytoplasm

KW - Female

KW - Ganglia, Spinal

KW - Immunohistochemistry

KW - In Situ Hybridization

KW - Interleukin-1

KW - Male

KW - Microscopy, Immunoelectron

KW - Neuroglia

KW - Neurons, Afferent

KW - Polyribosomes

KW - RNA, Messenger

KW - Rats

KW - Receptors, Interleukin-1

KW - Receptors, Interleukin-1 Type I

KW - S100 Calcium Binding Protein G

KW - Substance P

KW - Journal Article

M3 - Article

VL - 118

SP - 203

EP - 211

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

SN - 0165-5728

IS - 2

ER -

Copray JC, Mantingh I, Brouwer N, Biber K, Küst BM, Liem RS et al. Expression of interleukin-1 beta in rat dorsal root ganglia. Journal of Neuroimmunology. 2001 Aug 30;118(2):203-11.