Fabrication and characterization of poly(ε-caprolactone)/gelatin nanofibrous scaffolds for retinal tissue engineering

Shiva Rahmani, Fatemeh Tabandeh, Shahab Faghihi, Ghassem Amoabediny, Mehdi Shakibaie, Behnam Noorani, Fatemeh Yazdian

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Different ratios of poly(ε-caprolactone) (PCL) and gelatinwere used to fabricate scaffolds for regeneration of retinal pigment epithelium (RPE) layer. Physical and chemical characterizations were performed and the behavior of human RPE cells on the scaffolds was evaluated subsequently. An increase in gelatin content in the scaffold enhanced hydrophilicity, RPE cell attachment, proliferation, and spreading over PCL scaffolds. Granular and cytoplasmic expressions of RPE65 and Cytokeratin 8/18 markers confirmed the presence of RPE cells. It was believed that PCL/gelatin scaffolds could be used as substrates to replace RPE extracellular matrix to facilitate regeneration of RPE layer in retinal diseases.
Original languageEnglish
Pages (from-to)27-35
JournalINTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
Volume67
Issue number1
DOIs
Publication statusPublished - 2018
Externally publishedYes

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