Failure to detect functional neutrophil B helper cells in the human spleen

Sietse Quirijn Nagelkerke, Daan Jacob aan de Kerk, Machiel Hugo Jansen, Timo Kars van den Berg, Taco Willem Kuijpers

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

A novel role for human neutrophilic granulocytes was recently described, showing that these cells, upon entering the spleen, can be reprogrammed into a distinct B cell-helper neutrophil phenotype that is capable of eliciting B cell responses such as immunoglobulin secretion, class switch recombination and somatic hypermutation. Using similar protocols, we detected a homogeneous population of CD15(high)CD16(high) neutrophils in fresh human spleen samples, which did not differ in phenotype and function from blood neutrophils. No phenotypic characteristics of costimulatory nature were detected on splenic or circulating neutrophils, nor could we reproduce the immunoglobulin production of splenic B cells in the presence of splenic neutrophils, although B cell function and neutrophil activity were normal. Independent confirmation of a role for NBH cells is required.

Original languageEnglish
Pages (from-to)e88377
JournalPLoS ONE
Volume9
Issue number2
DOIs
Publication statusPublished - 2014

Cite this

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title = "Failure to detect functional neutrophil B helper cells in the human spleen",
abstract = "A novel role for human neutrophilic granulocytes was recently described, showing that these cells, upon entering the spleen, can be reprogrammed into a distinct B cell-helper neutrophil phenotype that is capable of eliciting B cell responses such as immunoglobulin secretion, class switch recombination and somatic hypermutation. Using similar protocols, we detected a homogeneous population of CD15(high)CD16(high) neutrophils in fresh human spleen samples, which did not differ in phenotype and function from blood neutrophils. No phenotypic characteristics of costimulatory nature were detected on splenic or circulating neutrophils, nor could we reproduce the immunoglobulin production of splenic B cells in the presence of splenic neutrophils, although B cell function and neutrophil activity were normal. Independent confirmation of a role for NBH cells is required.",
keywords = "B-Lymphocytes/cytology, Cell Differentiation, Cell Separation, Flow Cytometry, Humans, Immunoglobulins/immunology, Lewis X Antigen/metabolism, Neutrophils/cytology, Phenotype, Reactive Oxygen Species/metabolism, Receptors, IgG/metabolism, Spleen/cytology",
author = "Nagelkerke, {Sietse Quirijn} and {aan de Kerk}, {Daan Jacob} and Jansen, {Machiel Hugo} and {van den Berg}, {Timo Kars} and Kuijpers, {Taco Willem}",
year = "2014",
doi = "10.1371/journal.pone.0088377",
language = "English",
volume = "9",
pages = "e88377",
journal = "PLoS ONE",
issn = "1932-6203",
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Failure to detect functional neutrophil B helper cells in the human spleen. / Nagelkerke, Sietse Quirijn; aan de Kerk, Daan Jacob; Jansen, Machiel Hugo; van den Berg, Timo Kars; Kuijpers, Taco Willem.

In: PLoS ONE, Vol. 9, No. 2, 2014, p. e88377.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Failure to detect functional neutrophil B helper cells in the human spleen

AU - Nagelkerke, Sietse Quirijn

AU - aan de Kerk, Daan Jacob

AU - Jansen, Machiel Hugo

AU - van den Berg, Timo Kars

AU - Kuijpers, Taco Willem

PY - 2014

Y1 - 2014

N2 - A novel role for human neutrophilic granulocytes was recently described, showing that these cells, upon entering the spleen, can be reprogrammed into a distinct B cell-helper neutrophil phenotype that is capable of eliciting B cell responses such as immunoglobulin secretion, class switch recombination and somatic hypermutation. Using similar protocols, we detected a homogeneous population of CD15(high)CD16(high) neutrophils in fresh human spleen samples, which did not differ in phenotype and function from blood neutrophils. No phenotypic characteristics of costimulatory nature were detected on splenic or circulating neutrophils, nor could we reproduce the immunoglobulin production of splenic B cells in the presence of splenic neutrophils, although B cell function and neutrophil activity were normal. Independent confirmation of a role for NBH cells is required.

AB - A novel role for human neutrophilic granulocytes was recently described, showing that these cells, upon entering the spleen, can be reprogrammed into a distinct B cell-helper neutrophil phenotype that is capable of eliciting B cell responses such as immunoglobulin secretion, class switch recombination and somatic hypermutation. Using similar protocols, we detected a homogeneous population of CD15(high)CD16(high) neutrophils in fresh human spleen samples, which did not differ in phenotype and function from blood neutrophils. No phenotypic characteristics of costimulatory nature were detected on splenic or circulating neutrophils, nor could we reproduce the immunoglobulin production of splenic B cells in the presence of splenic neutrophils, although B cell function and neutrophil activity were normal. Independent confirmation of a role for NBH cells is required.

KW - B-Lymphocytes/cytology

KW - Cell Differentiation

KW - Cell Separation

KW - Flow Cytometry

KW - Humans

KW - Immunoglobulins/immunology

KW - Lewis X Antigen/metabolism

KW - Neutrophils/cytology

KW - Phenotype

KW - Reactive Oxygen Species/metabolism

KW - Receptors, IgG/metabolism

KW - Spleen/cytology

U2 - 10.1371/journal.pone.0088377

DO - 10.1371/journal.pone.0088377

M3 - Article

VL - 9

SP - e88377

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 2

ER -