Abstract
A novel role for human neutrophilic granulocytes was recently described, showing that these cells, upon entering the spleen, can be reprogrammed into a distinct B cell-helper neutrophil phenotype that is capable of eliciting B cell responses such as immunoglobulin secretion, class switch recombination and somatic hypermutation. Using similar protocols, we detected a homogeneous population of CD15(high)CD16(high) neutrophils in fresh human spleen samples, which did not differ in phenotype and function from blood neutrophils. No phenotypic characteristics of costimulatory nature were detected on splenic or circulating neutrophils, nor could we reproduce the immunoglobulin production of splenic B cells in the presence of splenic neutrophils, although B cell function and neutrophil activity were normal. Independent confirmation of a role for NBH cells is required.
| Original language | English |
|---|---|
| Pages (from-to) | e88377 |
| Journal | PLoS ONE |
| Volume | 9 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2014 |
Cite this
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Failure to detect functional neutrophil B helper cells in the human spleen. / Nagelkerke, Sietse Quirijn; aan de Kerk, Daan Jacob; Jansen, Machiel Hugo; van den Berg, Timo Kars; Kuijpers, Taco Willem.
In: PLoS ONE, Vol. 9, No. 2, 2014, p. e88377.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Failure to detect functional neutrophil B helper cells in the human spleen
AU - Nagelkerke, Sietse Quirijn
AU - aan de Kerk, Daan Jacob
AU - Jansen, Machiel Hugo
AU - van den Berg, Timo Kars
AU - Kuijpers, Taco Willem
PY - 2014
Y1 - 2014
N2 - A novel role for human neutrophilic granulocytes was recently described, showing that these cells, upon entering the spleen, can be reprogrammed into a distinct B cell-helper neutrophil phenotype that is capable of eliciting B cell responses such as immunoglobulin secretion, class switch recombination and somatic hypermutation. Using similar protocols, we detected a homogeneous population of CD15(high)CD16(high) neutrophils in fresh human spleen samples, which did not differ in phenotype and function from blood neutrophils. No phenotypic characteristics of costimulatory nature were detected on splenic or circulating neutrophils, nor could we reproduce the immunoglobulin production of splenic B cells in the presence of splenic neutrophils, although B cell function and neutrophil activity were normal. Independent confirmation of a role for NBH cells is required.
AB - A novel role for human neutrophilic granulocytes was recently described, showing that these cells, upon entering the spleen, can be reprogrammed into a distinct B cell-helper neutrophil phenotype that is capable of eliciting B cell responses such as immunoglobulin secretion, class switch recombination and somatic hypermutation. Using similar protocols, we detected a homogeneous population of CD15(high)CD16(high) neutrophils in fresh human spleen samples, which did not differ in phenotype and function from blood neutrophils. No phenotypic characteristics of costimulatory nature were detected on splenic or circulating neutrophils, nor could we reproduce the immunoglobulin production of splenic B cells in the presence of splenic neutrophils, although B cell function and neutrophil activity were normal. Independent confirmation of a role for NBH cells is required.
KW - B-Lymphocytes/cytology
KW - Cell Differentiation
KW - Cell Separation
KW - Flow Cytometry
KW - Humans
KW - Immunoglobulins/immunology
KW - Lewis X Antigen/metabolism
KW - Neutrophils/cytology
KW - Phenotype
KW - Reactive Oxygen Species/metabolism
KW - Receptors, IgG/metabolism
KW - Spleen/cytology
U2 - 10.1371/journal.pone.0088377
DO - 10.1371/journal.pone.0088377
M3 - Article
VL - 9
SP - e88377
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 2
ER -