Fluoride prevents degranulation of the azurophilic and specific granules in electropermeabilized neutrophils

H W Niessen; G C Kessels; A J Verhoeven

Fluoride prevents degranulation of the azurophilic and specific granules in electropermeabilized neutrophils

H W Niessen, G C Kessels, A J Verhoeven

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The effect of AlF4- on the degranulation process in human neutrophils was investigated. In intact neutrophils, AlF4- induced degranulation, whereas in electropermeabilized neutrophils AlF4- did not stimulate degranulation. In electropermeabilized neutrophils, fluoride ions proved to be inhibitory for the degranulation induced by addition of Ca2+ and/or GTP-gamma-S. Another phosphatase inhibitor, okadaic acid, inhibited degranulation induced by Ca2+ or by GTP-gamma-S but not degranulation induced by the combination of Ca2+ plus GTP-gamma-S. It is concluded that under suboptimal conditions of stimulation with Ca2+ or GTP-gamma-S, protein dephosphorylation plays an important role in the degranulation response in human neutrophils.

Original language	English
Pages (from-to)	489-95
Number of pages	7
Journal	Journal of Leukocyte Biology
Volume	55
Issue number	4
Publication status	Published - Apr 1994

Cite this

@article{93c8abb57fe04da193fef7a913db7130,

title = "Fluoride prevents degranulation of the azurophilic and specific granules in electropermeabilized neutrophils",

abstract = "The effect of AlF4- on the degranulation process in human neutrophils was investigated. In intact neutrophils, AlF4- induced degranulation, whereas in electropermeabilized neutrophils AlF4- did not stimulate degranulation. In electropermeabilized neutrophils, fluoride ions proved to be inhibitory for the degranulation induced by addition of Ca2+ and/or GTP-gamma-S. Another phosphatase inhibitor, okadaic acid, inhibited degranulation induced by Ca2+ or by GTP-gamma-S but not degranulation induced by the combination of Ca2+ plus GTP-gamma-S. It is concluded that under suboptimal conditions of stimulation with Ca2+ or GTP-gamma-S, protein dephosphorylation plays an important role in the degranulation response in human neutrophils.",

keywords = "Antigens, CD, Antigens, Neoplasm, Cell Adhesion Molecules, Cell Degranulation, Ethers, Cyclic, Fluorides, GTP-Binding Proteins, Guanosine 5'-O-(3-Thiotriphosphate), Humans, Membrane Glycoproteins, Neutrophils, Okadaic Acid, Permeability, Platelet Membrane Glycoproteins, Tetraspanin 30, Journal Article, Research Support, Non-U.S. Gov't",

author = "Niessen, {H W} and Kessels, {G C} and Verhoeven, {A J}",

year = "1994",

month = apr,

language = "English",

volume = "55",

pages = "489--95",

journal = "Journal of Leukocyte Biology",

issn = "0741-5400",

publisher = "FASEB",

number = "4",

}

TY - JOUR

T1 - Fluoride prevents degranulation of the azurophilic and specific granules in electropermeabilized neutrophils

AU - Niessen, H W

AU - Kessels, G C

AU - Verhoeven, A J

PY - 1994/4

Y1 - 1994/4

N2 - The effect of AlF4- on the degranulation process in human neutrophils was investigated. In intact neutrophils, AlF4- induced degranulation, whereas in electropermeabilized neutrophils AlF4- did not stimulate degranulation. In electropermeabilized neutrophils, fluoride ions proved to be inhibitory for the degranulation induced by addition of Ca2+ and/or GTP-gamma-S. Another phosphatase inhibitor, okadaic acid, inhibited degranulation induced by Ca2+ or by GTP-gamma-S but not degranulation induced by the combination of Ca2+ plus GTP-gamma-S. It is concluded that under suboptimal conditions of stimulation with Ca2+ or GTP-gamma-S, protein dephosphorylation plays an important role in the degranulation response in human neutrophils.

AB - The effect of AlF4- on the degranulation process in human neutrophils was investigated. In intact neutrophils, AlF4- induced degranulation, whereas in electropermeabilized neutrophils AlF4- did not stimulate degranulation. In electropermeabilized neutrophils, fluoride ions proved to be inhibitory for the degranulation induced by addition of Ca2+ and/or GTP-gamma-S. Another phosphatase inhibitor, okadaic acid, inhibited degranulation induced by Ca2+ or by GTP-gamma-S but not degranulation induced by the combination of Ca2+ plus GTP-gamma-S. It is concluded that under suboptimal conditions of stimulation with Ca2+ or GTP-gamma-S, protein dephosphorylation plays an important role in the degranulation response in human neutrophils.

KW - Antigens, CD

KW - Antigens, Neoplasm

KW - Cell Adhesion Molecules

KW - Cell Degranulation

KW - Ethers, Cyclic

KW - Fluorides

KW - GTP-Binding Proteins

KW - Guanosine 5'-O-(3-Thiotriphosphate)

KW - Humans

KW - Membrane Glycoproteins

KW - Neutrophils

KW - Okadaic Acid

KW - Permeability

KW - Platelet Membrane Glycoproteins

KW - Tetraspanin 30

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Article

C2 - 8145019

SN - 0741-5400

VL - 55

SP - 489

EP - 495

JO - Journal of Leukocyte Biology

JF - Journal of Leukocyte Biology

IS - 4

ER -