Functional CD169 on Macrophages Mediates Interaction with Dendritic Cells for CD8+ T Cell Cross-Priming

Dieke van Dinther, Henrike Veninga, Salvador Iborra, Ellen G F Borg, Leoni Hoogterp, Katarzyna Olesek, Marieke R Beijer, Sjoerd T T Schetters, Hakan Kalay, Juan J Garcia-Vallejo, Kees L Franken, Lamin B Cham, Karl S Lang, Yvette van Kooyk, David Sancho, Paul R Crocker, Joke M M den Haan

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Splenic CD169+ macrophages are located in the marginal zone to efficiently capture blood-borne pathogens. Here, we investigate the requirements for the induction of CD8+ T cell responses by antigens (Ags) bound by CD169+ macrophages. Upon Ag targeting to CD169+ macrophages, we show that BATF3-dependent CD8α+ dendritic cells (DCs) are crucial for DNGR-1-mediated cross-priming of CD8+ T cell responses. In addition, we demonstrate that CD169, a sialic acid binding lectin involved in cell-cell contact, preferentially binds to CD8α+ DCs and that Ag transfer to CD8α+ DCs and subsequent T cell activation is dependent on the sialic acid-binding capacity of CD169. Finally, functional CD169 mediates optimal CD8+ T cell responses to modified vaccinia Ankara virus infection. Together, these data indicate that the collaboration of CD169+ macrophages and CD8α+ DCs for the initiation of effective CD8+ T cell responses is facilitated by binding of CD169 to sialic acid containing ligands on CD8α+ DCs.

Original languageEnglish
Pages (from-to)1484-1495
Number of pages12
JournalCell Reports
Volume22
Issue number6
DOIs
Publication statusPublished - 6 Feb 2018

Cite this

van Dinther, Dieke ; Veninga, Henrike ; Iborra, Salvador ; Borg, Ellen G F ; Hoogterp, Leoni ; Olesek, Katarzyna ; Beijer, Marieke R ; Schetters, Sjoerd T T ; Kalay, Hakan ; Garcia-Vallejo, Juan J ; Franken, Kees L ; Cham, Lamin B ; Lang, Karl S ; van Kooyk, Yvette ; Sancho, David ; Crocker, Paul R ; den Haan, Joke M M. / Functional CD169 on Macrophages Mediates Interaction with Dendritic Cells for CD8+ T Cell Cross-Priming. In: Cell Reports. 2018 ; Vol. 22, No. 6. pp. 1484-1495.
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title = "Functional CD169 on Macrophages Mediates Interaction with Dendritic Cells for CD8+ T Cell Cross-Priming",
abstract = "Splenic CD169+ macrophages are located in the marginal zone to efficiently capture blood-borne pathogens. Here, we investigate the requirements for the induction of CD8+ T cell responses by antigens (Ags) bound by CD169+ macrophages. Upon Ag targeting to CD169+ macrophages, we show that BATF3-dependent CD8α+ dendritic cells (DCs) are crucial for DNGR-1-mediated cross-priming of CD8+ T cell responses. In addition, we demonstrate that CD169, a sialic acid binding lectin involved in cell-cell contact, preferentially binds to CD8α+ DCs and that Ag transfer to CD8α+ DCs and subsequent T cell activation is dependent on the sialic acid-binding capacity of CD169. Finally, functional CD169 mediates optimal CD8+ T cell responses to modified vaccinia Ankara virus infection. Together, these data indicate that the collaboration of CD169+ macrophages and CD8α+ DCs for the initiation of effective CD8+ T cell responses is facilitated by binding of CD169 to sialic acid containing ligands on CD8α+ DCs.",
author = "{van Dinther}, Dieke and Henrike Veninga and Salvador Iborra and Borg, {Ellen G F} and Leoni Hoogterp and Katarzyna Olesek and Beijer, {Marieke R} and Schetters, {Sjoerd T T} and Hakan Kalay and Garcia-Vallejo, {Juan J} and Franken, {Kees L} and Cham, {Lamin B} and Lang, {Karl S} and {van Kooyk}, Yvette and David Sancho and Crocker, {Paul R} and {den Haan}, {Joke M M}",
note = "Copyright {\circledC} 2018 The Authors. Published by Elsevier Inc. All rights reserved.",
year = "2018",
month = "2",
day = "6",
doi = "10.1016/j.celrep.2018.01.021",
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van Dinther, D, Veninga, H, Iborra, S, Borg, EGF, Hoogterp, L, Olesek, K, Beijer, MR, Schetters, STT, Kalay, H, Garcia-Vallejo, JJ, Franken, KL, Cham, LB, Lang, KS, van Kooyk, Y, Sancho, D, Crocker, PR & den Haan, JMM 2018, 'Functional CD169 on Macrophages Mediates Interaction with Dendritic Cells for CD8+ T Cell Cross-Priming' Cell Reports, vol. 22, no. 6, pp. 1484-1495. https://doi.org/10.1016/j.celrep.2018.01.021

Functional CD169 on Macrophages Mediates Interaction with Dendritic Cells for CD8+ T Cell Cross-Priming. / van Dinther, Dieke; Veninga, Henrike; Iborra, Salvador; Borg, Ellen G F; Hoogterp, Leoni; Olesek, Katarzyna; Beijer, Marieke R; Schetters, Sjoerd T T; Kalay, Hakan; Garcia-Vallejo, Juan J; Franken, Kees L; Cham, Lamin B; Lang, Karl S; van Kooyk, Yvette; Sancho, David; Crocker, Paul R; den Haan, Joke M M.

In: Cell Reports, Vol. 22, No. 6, 06.02.2018, p. 1484-1495.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Functional CD169 on Macrophages Mediates Interaction with Dendritic Cells for CD8+ T Cell Cross-Priming

AU - van Dinther, Dieke

AU - Veninga, Henrike

AU - Iborra, Salvador

AU - Borg, Ellen G F

AU - Hoogterp, Leoni

AU - Olesek, Katarzyna

AU - Beijer, Marieke R

AU - Schetters, Sjoerd T T

AU - Kalay, Hakan

AU - Garcia-Vallejo, Juan J

AU - Franken, Kees L

AU - Cham, Lamin B

AU - Lang, Karl S

AU - van Kooyk, Yvette

AU - Sancho, David

AU - Crocker, Paul R

AU - den Haan, Joke M M

N1 - Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.

PY - 2018/2/6

Y1 - 2018/2/6

N2 - Splenic CD169+ macrophages are located in the marginal zone to efficiently capture blood-borne pathogens. Here, we investigate the requirements for the induction of CD8+ T cell responses by antigens (Ags) bound by CD169+ macrophages. Upon Ag targeting to CD169+ macrophages, we show that BATF3-dependent CD8α+ dendritic cells (DCs) are crucial for DNGR-1-mediated cross-priming of CD8+ T cell responses. In addition, we demonstrate that CD169, a sialic acid binding lectin involved in cell-cell contact, preferentially binds to CD8α+ DCs and that Ag transfer to CD8α+ DCs and subsequent T cell activation is dependent on the sialic acid-binding capacity of CD169. Finally, functional CD169 mediates optimal CD8+ T cell responses to modified vaccinia Ankara virus infection. Together, these data indicate that the collaboration of CD169+ macrophages and CD8α+ DCs for the initiation of effective CD8+ T cell responses is facilitated by binding of CD169 to sialic acid containing ligands on CD8α+ DCs.

AB - Splenic CD169+ macrophages are located in the marginal zone to efficiently capture blood-borne pathogens. Here, we investigate the requirements for the induction of CD8+ T cell responses by antigens (Ags) bound by CD169+ macrophages. Upon Ag targeting to CD169+ macrophages, we show that BATF3-dependent CD8α+ dendritic cells (DCs) are crucial for DNGR-1-mediated cross-priming of CD8+ T cell responses. In addition, we demonstrate that CD169, a sialic acid binding lectin involved in cell-cell contact, preferentially binds to CD8α+ DCs and that Ag transfer to CD8α+ DCs and subsequent T cell activation is dependent on the sialic acid-binding capacity of CD169. Finally, functional CD169 mediates optimal CD8+ T cell responses to modified vaccinia Ankara virus infection. Together, these data indicate that the collaboration of CD169+ macrophages and CD8α+ DCs for the initiation of effective CD8+ T cell responses is facilitated by binding of CD169 to sialic acid containing ligands on CD8α+ DCs.

U2 - 10.1016/j.celrep.2018.01.021

DO - 10.1016/j.celrep.2018.01.021

M3 - Article

VL - 22

SP - 1484

EP - 1495

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 6

ER -

van Dinther D, Veninga H, Iborra S, Borg EGF, Hoogterp L, Olesek K et al. Functional CD169 on Macrophages Mediates Interaction with Dendritic Cells for CD8+ T Cell Cross-Priming. Cell Reports. 2018 Feb 6;22(6):1484-1495. https://doi.org/10.1016/j.celrep.2018.01.021