Funnel-freezing versus heat-stabilization for the visualization of metabolites by mass spectrometry imaging in a mouse stroke model

Inge A. Mulder, Clara Esteve, Marieke J.H. Wermer, Mathias Hoehn, Else A. Tolner, Arn M.J.M. van den Maagdenberg, Liam A. McDonnell*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


Tissue preparation is the key to a successful matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) experiment. Rapid post-mortem changes contribute a significant challenge to the use of MSI approaches for the analysis of peptides and metabolites. In this technical note we aimed to compare the tissue fixation method ex-vivo heat-stabilization with in-situ funnel-freezing in a middle cerebral artery occlusion (MCAo) mouse model of stroke, which causes profound alterations in metabolite concentrations. The influence of the duration of the thaw-mounting of the tissue sections on metabolite stability was also determined. We demonstrate improved stability and biomolecule visualization when funnel-freezing was used to sacrifice the mouse compared with heat-stabilization. Results were further improved when funnel-freezing was combined with fast thaw-mounting of the brain sections.

Original languageEnglish
Pages (from-to)1652-1659
Number of pages8
Issue number11-12
Publication statusPublished - 1 Jun 2016
Externally publishedYes

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