Funnel-freezing versus heat-stabilization for the visualization of metabolites by mass spectrometry imaging in a mouse stroke model

Inge A. Mulder, Clara Esteve, Marieke J.H. Wermer, Mathias Hoehn, Else A. Tolner, Arn M.J.M. van den Maagdenberg, Liam A. McDonnell*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Tissue preparation is the key to a successful matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) experiment. Rapid post-mortem changes contribute a significant challenge to the use of MSI approaches for the analysis of peptides and metabolites. In this technical note we aimed to compare the tissue fixation method ex-vivo heat-stabilization with in-situ funnel-freezing in a middle cerebral artery occlusion (MCAo) mouse model of stroke, which causes profound alterations in metabolite concentrations. The influence of the duration of the thaw-mounting of the tissue sections on metabolite stability was also determined. We demonstrate improved stability and biomolecule visualization when funnel-freezing was used to sacrifice the mouse compared with heat-stabilization. Results were further improved when funnel-freezing was combined with fast thaw-mounting of the brain sections.

Original languageEnglish
Pages (from-to)1652-1659
Number of pages8
JournalProteomics
Volume16
Issue number11-12
DOIs
Publication statusPublished - 1 Jun 2016

Cite this

Mulder, I. A., Esteve, C., Wermer, M. J. H., Hoehn, M., Tolner, E. A., van den Maagdenberg, A. M. J. M., & McDonnell, L. A. (2016). Funnel-freezing versus heat-stabilization for the visualization of metabolites by mass spectrometry imaging in a mouse stroke model. Proteomics, 16(11-12), 1652-1659. https://doi.org/10.1002/pmic.201500402