TY - JOUR
T1 - Gadolinium–DTPA–enhanced magnetic resonance imaging of the isolated rat heart after ischemia and reperfusion
AU - de Roos, Albert
AU - Mohanlal, Ramon W.
AU - van Vaals, Joop J.
AU - Bergman, Anthonie H.
AU - Doornbos, Joost
AU - Matheijssen, Niels A.A.
AU - van der Wall, Ernst E.
AU - van der Larse, Arnoud
PY - 1991/1/1
Y1 - 1991/1/1
N2 - de Roos A, Mohanlal RW, van Vaals JJ, et al. Gadolinium-DTPA-enhanced magnetic resonance imaging of the isolated rat heart after ischemia and reperfusion. Invest Radiol 1991; 26:1060-1064. The objective of this study was to assess the potential of gad-olinium-diethylenetriamine pentaacetic acid (Gd-DTPA) to identify myocardial ischemia and reperfusion in the isolated rat heart model. Ischemia was induced by reducing the perfusion pressure from 80 to 30 mm Hg for 2 hours. Hearts were not reperfused, or were reperfused for 20 minutes or for 2 hours. Perfusion was performed with Evans blue dye and/or Gd-DTPA for 3 minutes. Twenty isolated rat hearts were perfused according to the Langendorff method, and divided into five groups according to the perfusion status and the use of Gd-DTPA and/or Evans blue as perfusion markers. The Evans blue distribution in the hearts was assessed by point-counting volumetry. The Gd-DTPA distribution was assessed by magnetic resonance microimaging at 6.3 T field strength. Evans blue staining clearly identified areas with “no flow” or “no reflow.” Perfusion with Gd-DTPA enhanced signal intensity significantly, both in ischemic and reperfused myocardium. Signal intensity in hearts reperfused for 2 hours was increased significantly compared to nonreperfused ischemic hearts, but not to ischemic hearts reperfused for 20 minutes. Magnetic resonance imaging with the aid of Gd-DTPA can identify ischemia and reperfusion in the isolated rat heart, dependent on residual perfusion.
AB - de Roos A, Mohanlal RW, van Vaals JJ, et al. Gadolinium-DTPA-enhanced magnetic resonance imaging of the isolated rat heart after ischemia and reperfusion. Invest Radiol 1991; 26:1060-1064. The objective of this study was to assess the potential of gad-olinium-diethylenetriamine pentaacetic acid (Gd-DTPA) to identify myocardial ischemia and reperfusion in the isolated rat heart model. Ischemia was induced by reducing the perfusion pressure from 80 to 30 mm Hg for 2 hours. Hearts were not reperfused, or were reperfused for 20 minutes or for 2 hours. Perfusion was performed with Evans blue dye and/or Gd-DTPA for 3 minutes. Twenty isolated rat hearts were perfused according to the Langendorff method, and divided into five groups according to the perfusion status and the use of Gd-DTPA and/or Evans blue as perfusion markers. The Evans blue distribution in the hearts was assessed by point-counting volumetry. The Gd-DTPA distribution was assessed by magnetic resonance microimaging at 6.3 T field strength. Evans blue staining clearly identified areas with “no flow” or “no reflow.” Perfusion with Gd-DTPA enhanced signal intensity significantly, both in ischemic and reperfused myocardium. Signal intensity in hearts reperfused for 2 hours was increased significantly compared to nonreperfused ischemic hearts, but not to ischemic hearts reperfused for 20 minutes. Magnetic resonance imaging with the aid of Gd-DTPA can identify ischemia and reperfusion in the isolated rat heart, dependent on residual perfusion.
KW - Contrast enhancement
KW - Gadolinium-DTPA
KW - Infarction
KW - Magnetic resonance studies
KW - Myocardium
UR - http://www.scopus.com/inward/record.url?scp=0025833734&partnerID=8YFLogxK
M3 - Article
C2 - 1765438
AN - SCOPUS:0025833734
SN - 0020-9996
VL - 26
SP - 1060
EP - 1064
JO - Invest Radiol
JF - Invest Radiol
IS - 12
ER -