Glucocorticoid receptor ligand binding in monocytic cells using a microplate assay

J Jansen, B Uitdehaag, J W Koper, T K van Den Berg

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Glucocorticoids have profound effects on macrophage function and are widely used as anti-inflammatory drugs. Glucocorticoids receptor (GR) ligand binding capacity is a major determinant of cellular glucocorticoid sensitivity. The number and affinity of GR can be measured in a whole cell binding assay using (3)H-dexamethasone. Here, we describe a rapid and simple microplate assay for GR measurement using the human promonocytic cell line THP-1.

Original languageEnglish
Pages (from-to)262-4
Number of pages3
JournalPathobiology
Volume67
Issue number5-6
DOIs
Publication statusPublished - 1999

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