H9c2 cardiomyoblasts produce thyroid hormone

C. Meischl, H.P. Buermans, T. Hazes, M.J. Zuidwijk, R.J.P. Musters, C. Boer, A. van Lingen, W.S. Simonides, M.A. Blankenstein, C. Dupuy, W.J. Paulus, C.E. Hack, C. Ris-Stalpers, D. Roos, H.W.M. Niessen

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Thyroid hormone acts on a wide range of tissues. In the cardiovascular system, thyroid hormone is an important regulator of cardiac function and cardiovascular hemodynamics. Although some early reports in the literature suggested an unknown extrathyroidal source of thyroid hormone, it is currently thought to be produced exclusively in the thyroid gland, a highly specialized organ with the sole function of generating, storing, and secreting thyroid hormone. Whereas most of the proteins necessary for thyroid hormone synthesis are thought to be expressed exclusively in the thyroid gland, we now have found evidence that all of these proteins, i.e., thyroglobulin, DUOX1, DUOX2, the sodium-iodide symporter, pendrin, thyroid peroxidase, and thyroid-stimulating hormone receptor, are also expressed in cardiomyocytes. Furthermore, we found thyroglobulin to be transiently upregulated in an in vitro model of ischemia. When performing these experiments in the presence of 125 I, we found that 125 I was integrated into thyroglobulin and that under ischemia-like conditions the radioactive signal in thyroglobulin was reduced. Concomitantly we observed an increase of intracellularly produced, 125 I-labeled thyroid hormone. In conclusion, our findings demonstrate for the first time that cardiomyocytes produce thyroid hormone in a manner adapted to the cell's environment.

Original languageEnglish
Pages (from-to)C1227-C1233
JournalAmerican Journal of Physiology: Cell Physiology
Volume294
Issue number5
DOIs
Publication statusPublished - May 2008

Cite this

Meischl, C. ; Buermans, H.P. ; Hazes, T. ; Zuidwijk, M.J. ; Musters, R.J.P. ; Boer, C. ; van Lingen, A. ; Simonides, W.S. ; Blankenstein, M.A. ; Dupuy, C. ; Paulus, W.J. ; Hack, C.E. ; Ris-Stalpers, C. ; Roos, D. ; Niessen, H.W.M. / H9c2 cardiomyoblasts produce thyroid hormone. In: American Journal of Physiology: Cell Physiology. 2008 ; Vol. 294, No. 5. pp. C1227-C1233.
@article{7a7e1bdc0ff6454b9e0282387a3b68d9,
title = "H9c2 cardiomyoblasts produce thyroid hormone",
abstract = "Thyroid hormone acts on a wide range of tissues. In the cardiovascular system, thyroid hormone is an important regulator of cardiac function and cardiovascular hemodynamics. Although some early reports in the literature suggested an unknown extrathyroidal source of thyroid hormone, it is currently thought to be produced exclusively in the thyroid gland, a highly specialized organ with the sole function of generating, storing, and secreting thyroid hormone. Whereas most of the proteins necessary for thyroid hormone synthesis are thought to be expressed exclusively in the thyroid gland, we now have found evidence that all of these proteins, i.e., thyroglobulin, DUOX1, DUOX2, the sodium-iodide symporter, pendrin, thyroid peroxidase, and thyroid-stimulating hormone receptor, are also expressed in cardiomyocytes. Furthermore, we found thyroglobulin to be transiently upregulated in an in vitro model of ischemia. When performing these experiments in the presence of 125 I, we found that 125 I was integrated into thyroglobulin and that under ischemia-like conditions the radioactive signal in thyroglobulin was reduced. Concomitantly we observed an increase of intracellularly produced, 125 I-labeled thyroid hormone. In conclusion, our findings demonstrate for the first time that cardiomyocytes produce thyroid hormone in a manner adapted to the cell's environment.",
keywords = "Animals, Cell Culture Techniques, DNA, Complementary, Dual Oxidases, Flavoproteins, Iodine Radioisotopes, Male, Myocytes, Cardiac, NADPH Oxidases, Oligonucleotide Array Sequence Analysis, Polymerase Chain Reaction, RNA, Messenger, Rats, Rats, Wistar, Thyroglobulin, Thyroid Hormones, Journal Article, Research Support, Non-U.S. Gov't",
author = "C. Meischl and H.P. Buermans and T. Hazes and M.J. Zuidwijk and R.J.P. Musters and C. Boer and {van Lingen}, A. and W.S. Simonides and M.A. Blankenstein and C. Dupuy and W.J. Paulus and C.E. Hack and C. Ris-Stalpers and D. Roos and H.W.M. Niessen",
year = "2008",
month = "5",
doi = "10.1152/ajpcell.00328.2007",
language = "English",
volume = "294",
pages = "C1227--C1233",
journal = "American Journal of Physiology: Cell Physiology",
issn = "0363-6143",
publisher = "American Physiological Society",
number = "5",

}

H9c2 cardiomyoblasts produce thyroid hormone. / Meischl, C.; Buermans, H.P.; Hazes, T.; Zuidwijk, M.J.; Musters, R.J.P.; Boer, C.; van Lingen, A.; Simonides, W.S.; Blankenstein, M.A.; Dupuy, C.; Paulus, W.J.; Hack, C.E.; Ris-Stalpers, C.; Roos, D.; Niessen, H.W.M.

In: American Journal of Physiology: Cell Physiology, Vol. 294, No. 5, 05.2008, p. C1227-C1233.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - H9c2 cardiomyoblasts produce thyroid hormone

AU - Meischl, C.

AU - Buermans, H.P.

AU - Hazes, T.

AU - Zuidwijk, M.J.

AU - Musters, R.J.P.

AU - Boer, C.

AU - van Lingen, A.

AU - Simonides, W.S.

AU - Blankenstein, M.A.

AU - Dupuy, C.

AU - Paulus, W.J.

AU - Hack, C.E.

AU - Ris-Stalpers, C.

AU - Roos, D.

AU - Niessen, H.W.M.

PY - 2008/5

Y1 - 2008/5

N2 - Thyroid hormone acts on a wide range of tissues. In the cardiovascular system, thyroid hormone is an important regulator of cardiac function and cardiovascular hemodynamics. Although some early reports in the literature suggested an unknown extrathyroidal source of thyroid hormone, it is currently thought to be produced exclusively in the thyroid gland, a highly specialized organ with the sole function of generating, storing, and secreting thyroid hormone. Whereas most of the proteins necessary for thyroid hormone synthesis are thought to be expressed exclusively in the thyroid gland, we now have found evidence that all of these proteins, i.e., thyroglobulin, DUOX1, DUOX2, the sodium-iodide symporter, pendrin, thyroid peroxidase, and thyroid-stimulating hormone receptor, are also expressed in cardiomyocytes. Furthermore, we found thyroglobulin to be transiently upregulated in an in vitro model of ischemia. When performing these experiments in the presence of 125 I, we found that 125 I was integrated into thyroglobulin and that under ischemia-like conditions the radioactive signal in thyroglobulin was reduced. Concomitantly we observed an increase of intracellularly produced, 125 I-labeled thyroid hormone. In conclusion, our findings demonstrate for the first time that cardiomyocytes produce thyroid hormone in a manner adapted to the cell's environment.

AB - Thyroid hormone acts on a wide range of tissues. In the cardiovascular system, thyroid hormone is an important regulator of cardiac function and cardiovascular hemodynamics. Although some early reports in the literature suggested an unknown extrathyroidal source of thyroid hormone, it is currently thought to be produced exclusively in the thyroid gland, a highly specialized organ with the sole function of generating, storing, and secreting thyroid hormone. Whereas most of the proteins necessary for thyroid hormone synthesis are thought to be expressed exclusively in the thyroid gland, we now have found evidence that all of these proteins, i.e., thyroglobulin, DUOX1, DUOX2, the sodium-iodide symporter, pendrin, thyroid peroxidase, and thyroid-stimulating hormone receptor, are also expressed in cardiomyocytes. Furthermore, we found thyroglobulin to be transiently upregulated in an in vitro model of ischemia. When performing these experiments in the presence of 125 I, we found that 125 I was integrated into thyroglobulin and that under ischemia-like conditions the radioactive signal in thyroglobulin was reduced. Concomitantly we observed an increase of intracellularly produced, 125 I-labeled thyroid hormone. In conclusion, our findings demonstrate for the first time that cardiomyocytes produce thyroid hormone in a manner adapted to the cell's environment.

KW - Animals

KW - Cell Culture Techniques

KW - DNA, Complementary

KW - Dual Oxidases

KW - Flavoproteins

KW - Iodine Radioisotopes

KW - Male

KW - Myocytes, Cardiac

KW - NADPH Oxidases

KW - Oligonucleotide Array Sequence Analysis

KW - Polymerase Chain Reaction

KW - RNA, Messenger

KW - Rats

KW - Rats, Wistar

KW - Thyroglobulin

KW - Thyroid Hormones

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1152/ajpcell.00328.2007

DO - 10.1152/ajpcell.00328.2007

M3 - Article

VL - 294

SP - C1227-C1233

JO - American Journal of Physiology: Cell Physiology

JF - American Journal of Physiology: Cell Physiology

SN - 0363-6143

IS - 5

ER -