High frequency and diversity of parechovirus A in a cohort of Malawian children

Lieke Brouwer, Eveliina Karelehto, Alvin X. Han, Xiomara V. Thomas, Andrea H. L. Bruning, Job C. J. Calis, Michaël Boele van Hensbroek, Brenda M. Westerhuis, Darsha Amarthalingam, Sylvie M. Koekkoek, Sjoerd P. H. Rebers, Kamija S. Phiri, Katja C. Wolthers, Dasja Pajkrt

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Parechoviruses (PeVs) are highly prevalent viruses worldwide. Over the last decades, several studies have been published on PeV epidemiology in Europe, Asia and North America, while information on other continents is lacking. The aim of this study was to describe PeV circulation in a cohort of children in Malawi, Africa. A total of 749 stool samples obtained from Malawian children aged 6 to 60 months were tested for the presence of PeV by real-time PCR. We performed typing by phylogenetic and Basic Local Alignment Search Tool (BLAST) analysis. PeV was found in 57% of stool samples. Age was significantly associated with PeV positivity (p = 0.01). Typing by phylogenetic analysis resulted in 15 different types, while BLAST typing resulted in 14 different types and several indeterminate strains. In total, six strains showed inconsistencies in typing between the two methods. One strain, P02-4058, remained untypable by all methods, but appeared to belong to the recently reclassified PeV-A19 genotype. PeV-A1, -A2 and -A3 were the most prevalent types (26.8%, 13.8% and 9.8%, respectively). Both the prevalence and genetic diversity found in our study were remarkably high. Our data provide an important contribution to the scarce data available on PeV epidemiology in Africa.
Original languageEnglish
Pages (from-to)799-806
JournalArchives of virology
Volume164
Issue number3
DOIs
Publication statusPublished - 2019

Cite this

Brouwer, L., Karelehto, E., Han, A. X., Thomas, X. V., Bruning, A. H. L., Calis, J. C. J., ... Pajkrt, D. (2019). High frequency and diversity of parechovirus A in a cohort of Malawian children. Archives of virology, 164(3), 799-806. https://doi.org/10.1007/s00705-018-04131-7
Brouwer, Lieke ; Karelehto, Eveliina ; Han, Alvin X. ; Thomas, Xiomara V. ; Bruning, Andrea H. L. ; Calis, Job C. J. ; van Hensbroek, Michaël Boele ; Westerhuis, Brenda M. ; Amarthalingam, Darsha ; Koekkoek, Sylvie M. ; Rebers, Sjoerd P. H. ; Phiri, Kamija S. ; Wolthers, Katja C. ; Pajkrt, Dasja. / High frequency and diversity of parechovirus A in a cohort of Malawian children. In: Archives of virology. 2019 ; Vol. 164, No. 3. pp. 799-806.
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title = "High frequency and diversity of parechovirus A in a cohort of Malawian children",
abstract = "Parechoviruses (PeVs) are highly prevalent viruses worldwide. Over the last decades, several studies have been published on PeV epidemiology in Europe, Asia and North America, while information on other continents is lacking. The aim of this study was to describe PeV circulation in a cohort of children in Malawi, Africa. A total of 749 stool samples obtained from Malawian children aged 6 to 60 months were tested for the presence of PeV by real-time PCR. We performed typing by phylogenetic and Basic Local Alignment Search Tool (BLAST) analysis. PeV was found in 57{\%} of stool samples. Age was significantly associated with PeV positivity (p = 0.01). Typing by phylogenetic analysis resulted in 15 different types, while BLAST typing resulted in 14 different types and several indeterminate strains. In total, six strains showed inconsistencies in typing between the two methods. One strain, P02-4058, remained untypable by all methods, but appeared to belong to the recently reclassified PeV-A19 genotype. PeV-A1, -A2 and -A3 were the most prevalent types (26.8{\%}, 13.8{\%} and 9.8{\%}, respectively). Both the prevalence and genetic diversity found in our study were remarkably high. Our data provide an important contribution to the scarce data available on PeV epidemiology in Africa.",
author = "Lieke Brouwer and Eveliina Karelehto and Han, {Alvin X.} and Thomas, {Xiomara V.} and Bruning, {Andrea H. L.} and Calis, {Job C. J.} and {van Hensbroek}, {Micha{\"e}l Boele} and Westerhuis, {Brenda M.} and Darsha Amarthalingam and Koekkoek, {Sylvie M.} and Rebers, {Sjoerd P. H.} and Phiri, {Kamija S.} and Wolthers, {Katja C.} and Dasja Pajkrt",
year = "2019",
doi = "10.1007/s00705-018-04131-7",
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journal = "Archives of virology",
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Brouwer, L, Karelehto, E, Han, AX, Thomas, XV, Bruning, AHL, Calis, JCJ, van Hensbroek, MB, Westerhuis, BM, Amarthalingam, D, Koekkoek, SM, Rebers, SPH, Phiri, KS, Wolthers, KC & Pajkrt, D 2019, 'High frequency and diversity of parechovirus A in a cohort of Malawian children' Archives of virology, vol. 164, no. 3, pp. 799-806. https://doi.org/10.1007/s00705-018-04131-7

High frequency and diversity of parechovirus A in a cohort of Malawian children. / Brouwer, Lieke; Karelehto, Eveliina; Han, Alvin X.; Thomas, Xiomara V.; Bruning, Andrea H. L.; Calis, Job C. J.; van Hensbroek, Michaël Boele; Westerhuis, Brenda M.; Amarthalingam, Darsha; Koekkoek, Sylvie M.; Rebers, Sjoerd P. H.; Phiri, Kamija S.; Wolthers, Katja C.; Pajkrt, Dasja.

In: Archives of virology, Vol. 164, No. 3, 2019, p. 799-806.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - High frequency and diversity of parechovirus A in a cohort of Malawian children

AU - Brouwer, Lieke

AU - Karelehto, Eveliina

AU - Han, Alvin X.

AU - Thomas, Xiomara V.

AU - Bruning, Andrea H. L.

AU - Calis, Job C. J.

AU - van Hensbroek, Michaël Boele

AU - Westerhuis, Brenda M.

AU - Amarthalingam, Darsha

AU - Koekkoek, Sylvie M.

AU - Rebers, Sjoerd P. H.

AU - Phiri, Kamija S.

AU - Wolthers, Katja C.

AU - Pajkrt, Dasja

PY - 2019

Y1 - 2019

N2 - Parechoviruses (PeVs) are highly prevalent viruses worldwide. Over the last decades, several studies have been published on PeV epidemiology in Europe, Asia and North America, while information on other continents is lacking. The aim of this study was to describe PeV circulation in a cohort of children in Malawi, Africa. A total of 749 stool samples obtained from Malawian children aged 6 to 60 months were tested for the presence of PeV by real-time PCR. We performed typing by phylogenetic and Basic Local Alignment Search Tool (BLAST) analysis. PeV was found in 57% of stool samples. Age was significantly associated with PeV positivity (p = 0.01). Typing by phylogenetic analysis resulted in 15 different types, while BLAST typing resulted in 14 different types and several indeterminate strains. In total, six strains showed inconsistencies in typing between the two methods. One strain, P02-4058, remained untypable by all methods, but appeared to belong to the recently reclassified PeV-A19 genotype. PeV-A1, -A2 and -A3 were the most prevalent types (26.8%, 13.8% and 9.8%, respectively). Both the prevalence and genetic diversity found in our study were remarkably high. Our data provide an important contribution to the scarce data available on PeV epidemiology in Africa.

AB - Parechoviruses (PeVs) are highly prevalent viruses worldwide. Over the last decades, several studies have been published on PeV epidemiology in Europe, Asia and North America, while information on other continents is lacking. The aim of this study was to describe PeV circulation in a cohort of children in Malawi, Africa. A total of 749 stool samples obtained from Malawian children aged 6 to 60 months were tested for the presence of PeV by real-time PCR. We performed typing by phylogenetic and Basic Local Alignment Search Tool (BLAST) analysis. PeV was found in 57% of stool samples. Age was significantly associated with PeV positivity (p = 0.01). Typing by phylogenetic analysis resulted in 15 different types, while BLAST typing resulted in 14 different types and several indeterminate strains. In total, six strains showed inconsistencies in typing between the two methods. One strain, P02-4058, remained untypable by all methods, but appeared to belong to the recently reclassified PeV-A19 genotype. PeV-A1, -A2 and -A3 were the most prevalent types (26.8%, 13.8% and 9.8%, respectively). Both the prevalence and genetic diversity found in our study were remarkably high. Our data provide an important contribution to the scarce data available on PeV epidemiology in Africa.

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UR - https://www.ncbi.nlm.nih.gov/pubmed/30666460

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