Abstract
The current protocols for glycomic analysis of cells often require a large quantity of material (5-20 million cells). In order to analyze the N-glycosylation from small amounts of cells (≤1 million) as obtained from, for example, primary cell lines or cell sorting, and in a higher throughput approach, we set up a robust 96-well format PVDF-membrane based N-glycan release protocol followed by linkage-specific sialic acid stabilization, cleanup, and MALDI-TOF-MS analysis. We further evaluated the influence of PNGase F incubation time on the N-glycan profile.
Original language | English |
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Pages (from-to) | 185-196 |
Number of pages | 12 |
Journal | Methods in Molecular Biology |
Volume | 1503 |
DOIs | |
Publication status | Published - 2017 |