Abstract

Purpose: We previously found that homocysteine (Hcy)-induced apoptosis in endothelial cells coincided with increased NADPH oxidase (NOX) activity. In addition, in ischemic endothelial cells present in the heart, we showed that loss of serine protease dipeptidyl peptidase IV (DPP4) expression was correlated with induction of tissue factor (TF) expression. Since Hcy can initiate thrombosis through the induction of TF expression, in this study, we evaluated whether the inverse relation of TF and DPP4 is also Hcy-dependent and whether NOX-mediated reactive oxygen species (ROS) is playing a role herein. Methods: Human umbilical vein endothelial cells (HUVECs) were incubated with 2.5 mM Hcy for 3 and 6 h. The effects of Hcy on DPP4 and TF expression and NOX2/p47 phox -mediated nitrotyrosine (ROS) production were studied using digital-imaging microscopy. Results: In HUVECs, high levels of Hcy showed a significant increase of TF expression and a concomitant loss of DPP4 expression after 6 h. In addition, NOX subunits NOX2 and p47 phox were also significantly increased after 6 h of Hcy incubation and coincided with nitrotyrosine (ROS) expression. Interestingly, inhibition of NOX-mediated nitrotyrosine (ROS) with the use of apocynin not only reduced these effects, but also counteracted the effects of Hcy on TF and DPP4 expression. Conclusion: These results indicate that the inverse relation of TF and DPP4 in endothelial cells is also Hcy-dependent and related to NOX activity.
Original languageEnglish
Pages (from-to)29-38
Number of pages10
JournalPhysiology International
Volume106
Issue number1
Early online date19 Mar 2019
DOIs
Publication statusPublished - 2019

Cite this

@article{026ee05d6a7b45f0ba49d2190d8de23b,
title = "Homocysteine-induced inverse expression of tissue factor and DPP4 in endothelial cells is related to NADPH oxidase activity",
abstract = "Purpose: We previously found that homocysteine (Hcy)-induced apoptosis in endothelial cells coincided with increased NADPH oxidase (NOX) activity. In addition, in ischemic endothelial cells present in the heart, we showed that loss of serine protease dipeptidyl peptidase IV (DPP4) expression was correlated with induction of tissue factor (TF) expression. Since Hcy can initiate thrombosis through the induction of TF expression, in this study, we evaluated whether the inverse relation of TF and DPP4 is also Hcy-dependent and whether NOX-mediated reactive oxygen species (ROS) is playing a role herein. Methods: Human umbilical vein endothelial cells (HUVECs) were incubated with 2.5 mM Hcy for 3 and 6 h. The effects of Hcy on DPP4 and TF expression and NOX2/p47 phox -mediated nitrotyrosine (ROS) production were studied using digital-imaging microscopy. Results: In HUVECs, high levels of Hcy showed a significant increase of TF expression and a concomitant loss of DPP4 expression after 6 h. In addition, NOX subunits NOX2 and p47 phox were also significantly increased after 6 h of Hcy incubation and coincided with nitrotyrosine (ROS) expression. Interestingly, inhibition of NOX-mediated nitrotyrosine (ROS) with the use of apocynin not only reduced these effects, but also counteracted the effects of Hcy on TF and DPP4 expression. Conclusion: These results indicate that the inverse relation of TF and DPP4 in endothelial cells is also Hcy-dependent and related to NOX activity.",
keywords = "DPP4, Endothelial cells, Homocysteine, NADPH oxidase, Tissue factor",
author = "Korkmaz, {H. I.} and Hahn, {N. E.} and Jansen, {K. M.} and Musters, {R. J. P.} and {van Bezu}, J. and {van Wieringen}, {W. N.} and {van Zuijlen}, {P. P. M.} and Ulrich, {M. M. W.} and Niessen, {H. W. M.} and Krijnen, {P. A. J.}",
year = "2019",
doi = "10.1556/2060.106.2019.05",
language = "English",
volume = "106",
pages = "29--38",
journal = "Physiology International",
issn = "2498-602X",
publisher = "Akademiai Kiado",
number = "1",

}

TY - JOUR

T1 - Homocysteine-induced inverse expression of tissue factor and DPP4 in endothelial cells is related to NADPH oxidase activity

AU - Korkmaz, H. I.

AU - Hahn, N. E.

AU - Jansen, K. M.

AU - Musters, R. J. P.

AU - van Bezu, J.

AU - van Wieringen, W. N.

AU - van Zuijlen, P. P. M.

AU - Ulrich, M. M. W.

AU - Niessen, H. W. M.

AU - Krijnen, P. A. J.

PY - 2019

Y1 - 2019

N2 - Purpose: We previously found that homocysteine (Hcy)-induced apoptosis in endothelial cells coincided with increased NADPH oxidase (NOX) activity. In addition, in ischemic endothelial cells present in the heart, we showed that loss of serine protease dipeptidyl peptidase IV (DPP4) expression was correlated with induction of tissue factor (TF) expression. Since Hcy can initiate thrombosis through the induction of TF expression, in this study, we evaluated whether the inverse relation of TF and DPP4 is also Hcy-dependent and whether NOX-mediated reactive oxygen species (ROS) is playing a role herein. Methods: Human umbilical vein endothelial cells (HUVECs) were incubated with 2.5 mM Hcy for 3 and 6 h. The effects of Hcy on DPP4 and TF expression and NOX2/p47 phox -mediated nitrotyrosine (ROS) production were studied using digital-imaging microscopy. Results: In HUVECs, high levels of Hcy showed a significant increase of TF expression and a concomitant loss of DPP4 expression after 6 h. In addition, NOX subunits NOX2 and p47 phox were also significantly increased after 6 h of Hcy incubation and coincided with nitrotyrosine (ROS) expression. Interestingly, inhibition of NOX-mediated nitrotyrosine (ROS) with the use of apocynin not only reduced these effects, but also counteracted the effects of Hcy on TF and DPP4 expression. Conclusion: These results indicate that the inverse relation of TF and DPP4 in endothelial cells is also Hcy-dependent and related to NOX activity.

AB - Purpose: We previously found that homocysteine (Hcy)-induced apoptosis in endothelial cells coincided with increased NADPH oxidase (NOX) activity. In addition, in ischemic endothelial cells present in the heart, we showed that loss of serine protease dipeptidyl peptidase IV (DPP4) expression was correlated with induction of tissue factor (TF) expression. Since Hcy can initiate thrombosis through the induction of TF expression, in this study, we evaluated whether the inverse relation of TF and DPP4 is also Hcy-dependent and whether NOX-mediated reactive oxygen species (ROS) is playing a role herein. Methods: Human umbilical vein endothelial cells (HUVECs) were incubated with 2.5 mM Hcy for 3 and 6 h. The effects of Hcy on DPP4 and TF expression and NOX2/p47 phox -mediated nitrotyrosine (ROS) production were studied using digital-imaging microscopy. Results: In HUVECs, high levels of Hcy showed a significant increase of TF expression and a concomitant loss of DPP4 expression after 6 h. In addition, NOX subunits NOX2 and p47 phox were also significantly increased after 6 h of Hcy incubation and coincided with nitrotyrosine (ROS) expression. Interestingly, inhibition of NOX-mediated nitrotyrosine (ROS) with the use of apocynin not only reduced these effects, but also counteracted the effects of Hcy on TF and DPP4 expression. Conclusion: These results indicate that the inverse relation of TF and DPP4 in endothelial cells is also Hcy-dependent and related to NOX activity.

KW - DPP4

KW - Endothelial cells

KW - Homocysteine

KW - NADPH oxidase

KW - Tissue factor

UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85063921991&origin=inward

UR - https://www.ncbi.nlm.nih.gov/pubmed/30888218

U2 - 10.1556/2060.106.2019.05

DO - 10.1556/2060.106.2019.05

M3 - Article

VL - 106

SP - 29

EP - 38

JO - Physiology International

JF - Physiology International

SN - 2498-602X

IS - 1

ER -