How to handle adsorption of cerebrospinal fluid amyloid β (1–42) in laboratory practice? Identifying problematic handlings and resolving the issue by use of the Aβ42/Aβ40 ratio

Eline Willemse, Kees van Uffelen, Britta Brix, Sebastiaan Engelborghs, Hugo Vanderstichele, Charlotte Teunissen

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Introduction We aimed to investigate factors defining amyloid β (1–42) (Aβ1–42) adsorption during preanalytical workup of cerebrospinal fluid (CSF). Methods CSF was transferred to new tubes ≤4 times. Variables tested were different polypropylene tube brands, volumes, CSF Aβ1–42 concentrations, incubation times, pipettes, vortex intensities, and other CSF proteins, including hyperphosphorylated tau and Interleukin 1 Receptor Accessory Protein (IL-1RAcP). An enquiry assessed the number of transfers in current practice. Results In diagnostic practice, the number of transfers varied between 1 and 3. Every tube transfer resulted in 5% loss of Aβ1–42 concentration, even 10% in small volumes. Adsorption was observed after 30 seconds and after contact with the pipette tip. Tube brand, vortexing, or continuous tube movement did not influence adsorption. Adsorption for Aβ1–40 was similar, resulting in stable Aβ1–42/Aβ1–40 ratios over multiple tube transfers. Discussion We confirmed that adsorption of CSF Aβ1–42 during preanalytical processing is an important confounder. However, use of the Aβ1–42/Aβ1–40 ratio overcomes this effect and can therefore contribute to increased diagnostic accuracy.

Original languageEnglish
Pages (from-to)885-892
Number of pages8
JournalAlzheimer's and Dementia
Volume13
Issue number8
DOIs
Publication statusPublished - 1 Aug 2017

Cite this

@article{34cde014658c42a6b659ca321f0c5b38,
title = "How to handle adsorption of cerebrospinal fluid amyloid β (1–42) in laboratory practice? Identifying problematic handlings and resolving the issue by use of the Aβ42/Aβ40 ratio",
abstract = "Introduction We aimed to investigate factors defining amyloid β (1–42) (Aβ1–42) adsorption during preanalytical workup of cerebrospinal fluid (CSF). Methods CSF was transferred to new tubes ≤4 times. Variables tested were different polypropylene tube brands, volumes, CSF Aβ1–42 concentrations, incubation times, pipettes, vortex intensities, and other CSF proteins, including hyperphosphorylated tau and Interleukin 1 Receptor Accessory Protein (IL-1RAcP). An enquiry assessed the number of transfers in current practice. Results In diagnostic practice, the number of transfers varied between 1 and 3. Every tube transfer resulted in 5{\%} loss of Aβ1–42 concentration, even 10{\%} in small volumes. Adsorption was observed after 30 seconds and after contact with the pipette tip. Tube brand, vortexing, or continuous tube movement did not influence adsorption. Adsorption for Aβ1–40 was similar, resulting in stable Aβ1–42/Aβ1–40 ratios over multiple tube transfers. Discussion We confirmed that adsorption of CSF Aβ1–42 during preanalytical processing is an important confounder. However, use of the Aβ1–42/Aβ1–40 ratio overcomes this effect and can therefore contribute to increased diagnostic accuracy.",
keywords = "Adsorption, Alzheimer's disease, Amyloid β (1–40), Amyloid β (1–42), Biobanking, Biomarkers, Cerebrospinal fluid, Preanalytical variation, Ratio Aβ/Aβ",
author = "Eline Willemse and {van Uffelen}, Kees and Britta Brix and Sebastiaan Engelborghs and Hugo Vanderstichele and Charlotte Teunissen",
year = "2017",
month = "8",
day = "1",
doi = "10.1016/j.jalz.2017.01.010",
language = "English",
volume = "13",
pages = "885--892",
journal = "Alzheimers & Dementia",
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publisher = "Elsevier",
number = "8",

}

How to handle adsorption of cerebrospinal fluid amyloid β (1–42) in laboratory practice? Identifying problematic handlings and resolving the issue by use of the Aβ42/Aβ40 ratio. / Willemse, Eline; van Uffelen, Kees; Brix, Britta; Engelborghs, Sebastiaan; Vanderstichele, Hugo; Teunissen, Charlotte.

In: Alzheimer's and Dementia, Vol. 13, No. 8, 01.08.2017, p. 885-892.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - How to handle adsorption of cerebrospinal fluid amyloid β (1–42) in laboratory practice? Identifying problematic handlings and resolving the issue by use of the Aβ42/Aβ40 ratio

AU - Willemse, Eline

AU - van Uffelen, Kees

AU - Brix, Britta

AU - Engelborghs, Sebastiaan

AU - Vanderstichele, Hugo

AU - Teunissen, Charlotte

PY - 2017/8/1

Y1 - 2017/8/1

N2 - Introduction We aimed to investigate factors defining amyloid β (1–42) (Aβ1–42) adsorption during preanalytical workup of cerebrospinal fluid (CSF). Methods CSF was transferred to new tubes ≤4 times. Variables tested were different polypropylene tube brands, volumes, CSF Aβ1–42 concentrations, incubation times, pipettes, vortex intensities, and other CSF proteins, including hyperphosphorylated tau and Interleukin 1 Receptor Accessory Protein (IL-1RAcP). An enquiry assessed the number of transfers in current practice. Results In diagnostic practice, the number of transfers varied between 1 and 3. Every tube transfer resulted in 5% loss of Aβ1–42 concentration, even 10% in small volumes. Adsorption was observed after 30 seconds and after contact with the pipette tip. Tube brand, vortexing, or continuous tube movement did not influence adsorption. Adsorption for Aβ1–40 was similar, resulting in stable Aβ1–42/Aβ1–40 ratios over multiple tube transfers. Discussion We confirmed that adsorption of CSF Aβ1–42 during preanalytical processing is an important confounder. However, use of the Aβ1–42/Aβ1–40 ratio overcomes this effect and can therefore contribute to increased diagnostic accuracy.

AB - Introduction We aimed to investigate factors defining amyloid β (1–42) (Aβ1–42) adsorption during preanalytical workup of cerebrospinal fluid (CSF). Methods CSF was transferred to new tubes ≤4 times. Variables tested were different polypropylene tube brands, volumes, CSF Aβ1–42 concentrations, incubation times, pipettes, vortex intensities, and other CSF proteins, including hyperphosphorylated tau and Interleukin 1 Receptor Accessory Protein (IL-1RAcP). An enquiry assessed the number of transfers in current practice. Results In diagnostic practice, the number of transfers varied between 1 and 3. Every tube transfer resulted in 5% loss of Aβ1–42 concentration, even 10% in small volumes. Adsorption was observed after 30 seconds and after contact with the pipette tip. Tube brand, vortexing, or continuous tube movement did not influence adsorption. Adsorption for Aβ1–40 was similar, resulting in stable Aβ1–42/Aβ1–40 ratios over multiple tube transfers. Discussion We confirmed that adsorption of CSF Aβ1–42 during preanalytical processing is an important confounder. However, use of the Aβ1–42/Aβ1–40 ratio overcomes this effect and can therefore contribute to increased diagnostic accuracy.

KW - Adsorption

KW - Alzheimer's disease

KW - Amyloid β (1–40)

KW - Amyloid β (1–42)

KW - Biobanking

KW - Biomarkers

KW - Cerebrospinal fluid

KW - Preanalytical variation

KW - Ratio Aβ/Aβ

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U2 - 10.1016/j.jalz.2017.01.010

DO - 10.1016/j.jalz.2017.01.010

M3 - Article

VL - 13

SP - 885

EP - 892

JO - Alzheimers & Dementia

JF - Alzheimers & Dementia

SN - 1552-5260

IS - 8

ER -