TY - JOUR
T1 - HPV16 variant analysis in primary and recurrent CIN2/3 lesions demonstrates presence of the same consensus variant
AU - van der Weele, Pascal
AU - King, Audrey J.
AU - Meijer, Chris J. L. M.
AU - Steenbergen, Renske D. M.
N1 - Copyright © 2019. Published by Elsevier B.V.
PY - 2019
Y1 - 2019
N2 - Introduction: Recurrent cervical intraepithelial lesions (rCIN2/3) after treatment of CIN2/3 occur in 5–15% of cases. rCIN2/3 can result from incomplete resection of CIN2/3, where the same HPV type and variant remains present. rCIN2/3 could also occur following a new infection with a different HPV variant of the same HPV type as the initial lesion. This study investigates HPV16 consensus variants in paired HPV16 positive scrapes from baseline CIN2/3 and rCIN2/3 lesions. Methods: Paired HPV16 positive cervical scrapes of women with CIN2/3 at baseline and rCIN2/3 6 or 12 months after treatment were selected for whole-genome amplification and Illumina sequencing. Sequences were compared and nucleotide changes over time were characterized. Results: From 14 paired samples, 10 had identical consensus variants in baseline CIN2/3 and rCIN2/3. Four paired samples showed one to three nucleotide variations at recurrent disease compared to baseline. Conclusion: Identical or nearly identical HPV16 consensus variants were found in scrapes of paired HPV16 positive baseline CIN2/3 and rCIN2/3 lesions after treatment, suggesting no need for HPV variant analysis when the same HPV type is found in both lesions. These results argue for either incomplete excision of baseline CIN2/3 or inability of clearance of the original HPV infection.
AB - Introduction: Recurrent cervical intraepithelial lesions (rCIN2/3) after treatment of CIN2/3 occur in 5–15% of cases. rCIN2/3 can result from incomplete resection of CIN2/3, where the same HPV type and variant remains present. rCIN2/3 could also occur following a new infection with a different HPV variant of the same HPV type as the initial lesion. This study investigates HPV16 consensus variants in paired HPV16 positive scrapes from baseline CIN2/3 and rCIN2/3 lesions. Methods: Paired HPV16 positive cervical scrapes of women with CIN2/3 at baseline and rCIN2/3 6 or 12 months after treatment were selected for whole-genome amplification and Illumina sequencing. Sequences were compared and nucleotide changes over time were characterized. Results: From 14 paired samples, 10 had identical consensus variants in baseline CIN2/3 and rCIN2/3. Four paired samples showed one to three nucleotide variations at recurrent disease compared to baseline. Conclusion: Identical or nearly identical HPV16 consensus variants were found in scrapes of paired HPV16 positive baseline CIN2/3 and rCIN2/3 lesions after treatment, suggesting no need for HPV variant analysis when the same HPV type is found in both lesions. These results argue for either incomplete excision of baseline CIN2/3 or inability of clearance of the original HPV infection.
KW - CIN
KW - HPV genome variants
KW - HPV16
KW - Recurrent infection
KW - Whole-genome sequencing
KW - rCIN
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85064449381&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/30991124
U2 - 10.1016/j.pvr.2019.04.008
DO - 10.1016/j.pvr.2019.04.008
M3 - Article
C2 - 30991124
VL - 7
SP - 168
EP - 172
JO - PAPILLOMAVIRUS RESEARCH
JF - PAPILLOMAVIRUS RESEARCH
SN - 2405-8521
ER -