HPV16 variant analysis in primary and recurrent CIN2/3 lesions demonstrates presence of the same consensus variant

Pascal van der Weele, Audrey J. King, Chris J. L. M. Meijer, Renske D. M. Steenbergen

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Introduction: Recurrent cervical intraepithelial lesions (rCIN2/3) after treatment of CIN2/3 occur in 5–15% of cases. rCIN2/3 can result from incomplete resection of CIN2/3, where the same HPV type and variant remains present. rCIN2/3 could also occur following a new infection with a different HPV variant of the same HPV type as the initial lesion. This study investigates HPV16 consensus variants in paired HPV16 positive scrapes from baseline CIN2/3 and rCIN2/3 lesions. Methods: Paired HPV16 positive cervical scrapes of women with CIN2/3 at baseline and rCIN2/3 6 or 12 months after treatment were selected for whole-genome amplification and Illumina sequencing. Sequences were compared and nucleotide changes over time were characterized. Results: From 14 paired samples, 10 had identical consensus variants in baseline CIN2/3 and rCIN2/3. Four paired samples showed one to three nucleotide variations at recurrent disease compared to baseline. Conclusion: Identical or nearly identical HPV16 consensus variants were found in scrapes of paired HPV16 positive baseline CIN2/3 and rCIN2/3 lesions after treatment, suggesting no need for HPV variant analysis when the same HPV type is found in both lesions. These results argue for either incomplete excision of baseline CIN2/3 or inability of clearance of the original HPV infection.
Original languageEnglish
Pages (from-to)168-172
Number of pages5
JournalPAPILLOMAVIRUS RESEARCH
Volume7
Early online date13 Apr 2019
DOIs
Publication statusPublished - 2019

Cite this

@article{a80f2f1f3b9949ba9b3701152a4ff328,
title = "HPV16 variant analysis in primary and recurrent CIN2/3 lesions demonstrates presence of the same consensus variant",
abstract = "Introduction: Recurrent cervical intraepithelial lesions (rCIN2/3) after treatment of CIN2/3 occur in 5–15{\%} of cases. rCIN2/3 can result from incomplete resection of CIN2/3, where the same HPV type and variant remains present. rCIN2/3 could also occur following a new infection with a different HPV variant of the same HPV type as the initial lesion. This study investigates HPV16 consensus variants in paired HPV16 positive scrapes from baseline CIN2/3 and rCIN2/3 lesions. Methods: Paired HPV16 positive cervical scrapes of women with CIN2/3 at baseline and rCIN2/3 6 or 12 months after treatment were selected for whole-genome amplification and Illumina sequencing. Sequences were compared and nucleotide changes over time were characterized. Results: From 14 paired samples, 10 had identical consensus variants in baseline CIN2/3 and rCIN2/3. Four paired samples showed one to three nucleotide variations at recurrent disease compared to baseline. Conclusion: Identical or nearly identical HPV16 consensus variants were found in scrapes of paired HPV16 positive baseline CIN2/3 and rCIN2/3 lesions after treatment, suggesting no need for HPV variant analysis when the same HPV type is found in both lesions. These results argue for either incomplete excision of baseline CIN2/3 or inability of clearance of the original HPV infection.",
author = "{van der Weele}, Pascal and King, {Audrey J.} and Meijer, {Chris J. L. M.} and Steenbergen, {Renske D. M.}",
note = "Copyright {\circledC} 2019. Published by Elsevier B.V.",
year = "2019",
doi = "10.1016/j.pvr.2019.04.008",
language = "English",
volume = "7",
pages = "168--172",
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HPV16 variant analysis in primary and recurrent CIN2/3 lesions demonstrates presence of the same consensus variant. / van der Weele, Pascal; King, Audrey J.; Meijer, Chris J. L. M.; Steenbergen, Renske D. M.

In: PAPILLOMAVIRUS RESEARCH, Vol. 7, 2019, p. 168-172.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - HPV16 variant analysis in primary and recurrent CIN2/3 lesions demonstrates presence of the same consensus variant

AU - van der Weele, Pascal

AU - King, Audrey J.

AU - Meijer, Chris J. L. M.

AU - Steenbergen, Renske D. M.

N1 - Copyright © 2019. Published by Elsevier B.V.

PY - 2019

Y1 - 2019

N2 - Introduction: Recurrent cervical intraepithelial lesions (rCIN2/3) after treatment of CIN2/3 occur in 5–15% of cases. rCIN2/3 can result from incomplete resection of CIN2/3, where the same HPV type and variant remains present. rCIN2/3 could also occur following a new infection with a different HPV variant of the same HPV type as the initial lesion. This study investigates HPV16 consensus variants in paired HPV16 positive scrapes from baseline CIN2/3 and rCIN2/3 lesions. Methods: Paired HPV16 positive cervical scrapes of women with CIN2/3 at baseline and rCIN2/3 6 or 12 months after treatment were selected for whole-genome amplification and Illumina sequencing. Sequences were compared and nucleotide changes over time were characterized. Results: From 14 paired samples, 10 had identical consensus variants in baseline CIN2/3 and rCIN2/3. Four paired samples showed one to three nucleotide variations at recurrent disease compared to baseline. Conclusion: Identical or nearly identical HPV16 consensus variants were found in scrapes of paired HPV16 positive baseline CIN2/3 and rCIN2/3 lesions after treatment, suggesting no need for HPV variant analysis when the same HPV type is found in both lesions. These results argue for either incomplete excision of baseline CIN2/3 or inability of clearance of the original HPV infection.

AB - Introduction: Recurrent cervical intraepithelial lesions (rCIN2/3) after treatment of CIN2/3 occur in 5–15% of cases. rCIN2/3 can result from incomplete resection of CIN2/3, where the same HPV type and variant remains present. rCIN2/3 could also occur following a new infection with a different HPV variant of the same HPV type as the initial lesion. This study investigates HPV16 consensus variants in paired HPV16 positive scrapes from baseline CIN2/3 and rCIN2/3 lesions. Methods: Paired HPV16 positive cervical scrapes of women with CIN2/3 at baseline and rCIN2/3 6 or 12 months after treatment were selected for whole-genome amplification and Illumina sequencing. Sequences were compared and nucleotide changes over time were characterized. Results: From 14 paired samples, 10 had identical consensus variants in baseline CIN2/3 and rCIN2/3. Four paired samples showed one to three nucleotide variations at recurrent disease compared to baseline. Conclusion: Identical or nearly identical HPV16 consensus variants were found in scrapes of paired HPV16 positive baseline CIN2/3 and rCIN2/3 lesions after treatment, suggesting no need for HPV variant analysis when the same HPV type is found in both lesions. These results argue for either incomplete excision of baseline CIN2/3 or inability of clearance of the original HPV infection.

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