Human NLRP3 inflammasome activation is Nox1-4 independent

Robin van Bruggen, M Yavuz Köker, Machiel Jansen, Michel van Houdt, Dirk Roos, Taco W Kuijpers, Timo K van den Berg

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The NLRP3 inflammasome can be activated by pathogen-associated molecular patterns or endogenous danger-associated molecular patterns. The activation of the NLRP3 inflammasome results in proteolytic activation and secretion of cytokines of the interleukin-1 (IL-1) family. The precise mode of activation of the NLRP3 inflammasome is still elusive, but has been postulated to be mediated by reactive oxygen species (ROS) generated by an NADPH oxidase. Using primary cells from chronic granulomatous disease (CGD) patients lacking expression of p22(phox), a protein that is required for the function of Nox1-4, we show that cells lacking NADPH oxidase activity are capable of secreting normal amounts of IL-1beta. Thus, we provide evidence that activation of the NLRP3 inflammasome does not depend on ROS generated from an NADPH oxidase.

Original languageEnglish
Pages (from-to)5398-400
Number of pages3
JournalBlood
Volume115
Issue number26
DOIs
Publication statusPublished - 1 Jul 2010

Cite this

van Bruggen, R., Köker, M. Y., Jansen, M., van Houdt, M., Roos, D., Kuijpers, T. W., & van den Berg, T. K. (2010). Human NLRP3 inflammasome activation is Nox1-4 independent. Blood, 115(26), 5398-400. https://doi.org/10.1182/blood-2009-10-250803