Hypofractionated radiation induces a decrease in cell proliferation but no histological damage to organotypic multicellular spheroids of human glioblastomas

P Kaaijk, D Troost, P Sminia, M C Hulshof, A H van der Kracht, S Leenstra, D A Bosch

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The aim of this study was to examine the effect of radiation on glioblastoma, using an organotypic multicellular spheroid (OMS) model. Most glioblastoma cell lines are, in contrast to glioblastomas in vivo, relatively radiosensitive. This limits the value of using cell lines for studying the radiation effect of glioblastomas. The advantage of OMS is maintenance of the characteristics of the original tumour, which is lost in conventional cell cultures. OMS prepared from four glioblastomas were treated with hypofractionated radiation with a radiobiologically equivalent dose to standard radiation treatment for glioblastoma patients. After treatment, the histology as well as the cell proliferation of the OMS was examined. After radiation, a significant decrease in cell proliferation was found, although no histological damage to the OMS was observed. The modest effects of radiation on the OMS are in agreement with the limited therapeutic value of radiotherapy for glioblastoma patients. Therefore, OMS seems to be a good alternative for cell lines to study the radiobiological effect on glioblastomas.

LanguageEnglish
Pages645-51
Number of pages7
JournalEuropean Journal of Cancer
Volume33
Issue number4
Publication statusPublished - Apr 1997

Cite this

Kaaijk, P ; Troost, D ; Sminia, P ; Hulshof, M C ; van der Kracht, A H ; Leenstra, S ; Bosch, D A. / Hypofractionated radiation induces a decrease in cell proliferation but no histological damage to organotypic multicellular spheroids of human glioblastomas. In: European Journal of Cancer. 1997 ; Vol. 33, No. 4. pp. 645-51.
@article{75e766f4624a444d8c6457f895308580,
title = "Hypofractionated radiation induces a decrease in cell proliferation but no histological damage to organotypic multicellular spheroids of human glioblastomas",
abstract = "The aim of this study was to examine the effect of radiation on glioblastoma, using an organotypic multicellular spheroid (OMS) model. Most glioblastoma cell lines are, in contrast to glioblastomas in vivo, relatively radiosensitive. This limits the value of using cell lines for studying the radiation effect of glioblastomas. The advantage of OMS is maintenance of the characteristics of the original tumour, which is lost in conventional cell cultures. OMS prepared from four glioblastomas were treated with hypofractionated radiation with a radiobiologically equivalent dose to standard radiation treatment for glioblastoma patients. After treatment, the histology as well as the cell proliferation of the OMS was examined. After radiation, a significant decrease in cell proliferation was found, although no histological damage to the OMS was observed. The modest effects of radiation on the OMS are in agreement with the limited therapeutic value of radiotherapy for glioblastoma patients. Therefore, OMS seems to be a good alternative for cell lines to study the radiobiological effect on glioblastomas.",
keywords = "Cell Division/radiation effects, Glioblastoma/chemistry, Humans, Image Processing, Computer-Assisted, Immunohistochemistry, Radiation Dosage, Spheroids, Cellular/chemistry, Tumor Suppressor Protein p53/analysis",
author = "P Kaaijk and D Troost and P Sminia and Hulshof, {M C} and {van der Kracht}, {A H} and S Leenstra and Bosch, {D A}",
year = "1997",
month = "4",
language = "English",
volume = "33",
pages = "645--51",
journal = "European Journal of Cancer",
issn = "0959-8049",
publisher = "Pergamon",
number = "4",

}

Hypofractionated radiation induces a decrease in cell proliferation but no histological damage to organotypic multicellular spheroids of human glioblastomas. / Kaaijk, P; Troost, D; Sminia, P; Hulshof, M C; van der Kracht, A H; Leenstra, S; Bosch, D A.

In: European Journal of Cancer, Vol. 33, No. 4, 04.1997, p. 645-51.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Hypofractionated radiation induces a decrease in cell proliferation but no histological damage to organotypic multicellular spheroids of human glioblastomas

AU - Kaaijk, P

AU - Troost, D

AU - Sminia, P

AU - Hulshof, M C

AU - van der Kracht, A H

AU - Leenstra, S

AU - Bosch, D A

PY - 1997/4

Y1 - 1997/4

N2 - The aim of this study was to examine the effect of radiation on glioblastoma, using an organotypic multicellular spheroid (OMS) model. Most glioblastoma cell lines are, in contrast to glioblastomas in vivo, relatively radiosensitive. This limits the value of using cell lines for studying the radiation effect of glioblastomas. The advantage of OMS is maintenance of the characteristics of the original tumour, which is lost in conventional cell cultures. OMS prepared from four glioblastomas were treated with hypofractionated radiation with a radiobiologically equivalent dose to standard radiation treatment for glioblastoma patients. After treatment, the histology as well as the cell proliferation of the OMS was examined. After radiation, a significant decrease in cell proliferation was found, although no histological damage to the OMS was observed. The modest effects of radiation on the OMS are in agreement with the limited therapeutic value of radiotherapy for glioblastoma patients. Therefore, OMS seems to be a good alternative for cell lines to study the radiobiological effect on glioblastomas.

AB - The aim of this study was to examine the effect of radiation on glioblastoma, using an organotypic multicellular spheroid (OMS) model. Most glioblastoma cell lines are, in contrast to glioblastomas in vivo, relatively radiosensitive. This limits the value of using cell lines for studying the radiation effect of glioblastomas. The advantage of OMS is maintenance of the characteristics of the original tumour, which is lost in conventional cell cultures. OMS prepared from four glioblastomas were treated with hypofractionated radiation with a radiobiologically equivalent dose to standard radiation treatment for glioblastoma patients. After treatment, the histology as well as the cell proliferation of the OMS was examined. After radiation, a significant decrease in cell proliferation was found, although no histological damage to the OMS was observed. The modest effects of radiation on the OMS are in agreement with the limited therapeutic value of radiotherapy for glioblastoma patients. Therefore, OMS seems to be a good alternative for cell lines to study the radiobiological effect on glioblastomas.

KW - Cell Division/radiation effects

KW - Glioblastoma/chemistry

KW - Humans

KW - Image Processing, Computer-Assisted

KW - Immunohistochemistry

KW - Radiation Dosage

KW - Spheroids, Cellular/chemistry

KW - Tumor Suppressor Protein p53/analysis

M3 - Article

VL - 33

SP - 645

EP - 651

JO - European Journal of Cancer

T2 - European Journal of Cancer

JF - European Journal of Cancer

SN - 0959-8049

IS - 4

ER -