Identification of a 43‐kda nuclear antigen associated with proliferation by monoclonal antibody k 112

Jasper J. Quak*, Guus Van Dongen, Marie A.E. Koken, Joost G.P. Brakkee, Rik J. Scheper, Alfons J.M. Balm, Luitzen De Jong, Gordon B. Snow, Chris J.L.M. Meijer

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


Monoclonal antibody (MAb) K 112 was generated after a single intrasplenic immunization with a recurrent laryngeal squamous‐cell carcinoma. The antibody detects a 43‐kDa nuclear antigen, with a pl of 5.4, which is expressed only in cycling cells. Expression is typically seen in a granular pattern excluding the nucleoli. During mitosis the bulk of the antigen is diffusely distributed in the cytoplasm. Identical reactivity was observed for tissues or cells of all mammalian species tested. These data indicate that MAb K 112 recognizes a protein belonging to the class of cell‐cycle‐related nuclear antigen molecules.

Original languageEnglish
Pages (from-to)50-55
Number of pages6
JournalInternational Journal of Cancer
Issue number1
Publication statusPublished - 1 Jan 1990

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