IgG subclass response to Helicobacter pylori in patients with chronic active gastritis and duodenal ulcer

H J Bontkes, R A Veenendaal, A S Peña, J G Goedhard, W van Duijn, J Kuiper, Jan L Meijer, C B Lamers

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The IgG subclass response is determined by the type of bacteria producing the infection and by genetic factors of the host. Patients with a Helicobacter pylori infection develop a specific immune response that is mainly of the IgA and IgG class. We measured the IgG subclass response in 20 patients with chronic active gastritis without a history of duodenal ulcer and 20 patients with chronic active gastritis and duodenal ulcer diagnosed by endoscopy and histology. A control group included 20 H. pylori-negative patients and 60 H. pylori-positive blood transfusion donors. Systemic IgG subclass response was measured with a modified enzyme-linked immunosorbent assay technique, using as antigen a sonicate of six different H. pylori strains. Mouse monoclonal antibodies against each of the four human IgG subclasses (IgG1, IgG2, IgG3, and IgG4) were used. The total IgG anti-H. pylori antibody titres were equal in all three H. pylori-positive groups and significantly different from that of the negative control group (p less than 0.01). The IgG subclass response in persons infected with H. pylori involved all four subclasses but was predominantly of the IgG1 and IgG2 subclasses. All of the groups with H. pylori infection had significantly higher levels of IgG1 than the negative control group, but no differences were detected among the three groups. However, the duodenal ulcer group had a significantly higher IgG2 response than the gastritis group (mean optical density +/- SEM, 0.382 +/- 0.047 versus 0.200 +/- 0.025, respectively; p less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish
Pages (from-to)129-33
Number of pages5
JournalScandinavian Journal of Gastroenterology
Volume27
Issue number2
Publication statusPublished - 1992

Cite this

Bontkes, H J ; Veenendaal, R A ; Peña, A S ; Goedhard, J G ; van Duijn, W ; Kuiper, J ; Meijer, Jan L ; Lamers, C B. / IgG subclass response to Helicobacter pylori in patients with chronic active gastritis and duodenal ulcer. In: Scandinavian Journal of Gastroenterology. 1992 ; Vol. 27, No. 2. pp. 129-33.
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abstract = "The IgG subclass response is determined by the type of bacteria producing the infection and by genetic factors of the host. Patients with a Helicobacter pylori infection develop a specific immune response that is mainly of the IgA and IgG class. We measured the IgG subclass response in 20 patients with chronic active gastritis without a history of duodenal ulcer and 20 patients with chronic active gastritis and duodenal ulcer diagnosed by endoscopy and histology. A control group included 20 H. pylori-negative patients and 60 H. pylori-positive blood transfusion donors. Systemic IgG subclass response was measured with a modified enzyme-linked immunosorbent assay technique, using as antigen a sonicate of six different H. pylori strains. Mouse monoclonal antibodies against each of the four human IgG subclasses (IgG1, IgG2, IgG3, and IgG4) were used. The total IgG anti-H. pylori antibody titres were equal in all three H. pylori-positive groups and significantly different from that of the negative control group (p less than 0.01). The IgG subclass response in persons infected with H. pylori involved all four subclasses but was predominantly of the IgG1 and IgG2 subclasses. All of the groups with H. pylori infection had significantly higher levels of IgG1 than the negative control group, but no differences were detected among the three groups. However, the duodenal ulcer group had a significantly higher IgG2 response than the gastritis group (mean optical density +/- SEM, 0.382 +/- 0.047 versus 0.200 +/- 0.025, respectively; p less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)",
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Bontkes, HJ, Veenendaal, RA, Peña, AS, Goedhard, JG, van Duijn, W, Kuiper, J, Meijer, JL & Lamers, CB 1992, 'IgG subclass response to Helicobacter pylori in patients with chronic active gastritis and duodenal ulcer' Scandinavian Journal of Gastroenterology, vol. 27, no. 2, pp. 129-33.

IgG subclass response to Helicobacter pylori in patients with chronic active gastritis and duodenal ulcer. / Bontkes, H J; Veenendaal, R A; Peña, A S; Goedhard, J G; van Duijn, W; Kuiper, J; Meijer, Jan L; Lamers, C B.

In: Scandinavian Journal of Gastroenterology, Vol. 27, No. 2, 1992, p. 129-33.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - IgG subclass response to Helicobacter pylori in patients with chronic active gastritis and duodenal ulcer

AU - Bontkes, H J

AU - Veenendaal, R A

AU - Peña, A S

AU - Goedhard, J G

AU - van Duijn, W

AU - Kuiper, J

AU - Meijer, Jan L

AU - Lamers, C B

PY - 1992

Y1 - 1992

N2 - The IgG subclass response is determined by the type of bacteria producing the infection and by genetic factors of the host. Patients with a Helicobacter pylori infection develop a specific immune response that is mainly of the IgA and IgG class. We measured the IgG subclass response in 20 patients with chronic active gastritis without a history of duodenal ulcer and 20 patients with chronic active gastritis and duodenal ulcer diagnosed by endoscopy and histology. A control group included 20 H. pylori-negative patients and 60 H. pylori-positive blood transfusion donors. Systemic IgG subclass response was measured with a modified enzyme-linked immunosorbent assay technique, using as antigen a sonicate of six different H. pylori strains. Mouse monoclonal antibodies against each of the four human IgG subclasses (IgG1, IgG2, IgG3, and IgG4) were used. The total IgG anti-H. pylori antibody titres were equal in all three H. pylori-positive groups and significantly different from that of the negative control group (p less than 0.01). The IgG subclass response in persons infected with H. pylori involved all four subclasses but was predominantly of the IgG1 and IgG2 subclasses. All of the groups with H. pylori infection had significantly higher levels of IgG1 than the negative control group, but no differences were detected among the three groups. However, the duodenal ulcer group had a significantly higher IgG2 response than the gastritis group (mean optical density +/- SEM, 0.382 +/- 0.047 versus 0.200 +/- 0.025, respectively; p less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)

AB - The IgG subclass response is determined by the type of bacteria producing the infection and by genetic factors of the host. Patients with a Helicobacter pylori infection develop a specific immune response that is mainly of the IgA and IgG class. We measured the IgG subclass response in 20 patients with chronic active gastritis without a history of duodenal ulcer and 20 patients with chronic active gastritis and duodenal ulcer diagnosed by endoscopy and histology. A control group included 20 H. pylori-negative patients and 60 H. pylori-positive blood transfusion donors. Systemic IgG subclass response was measured with a modified enzyme-linked immunosorbent assay technique, using as antigen a sonicate of six different H. pylori strains. Mouse monoclonal antibodies against each of the four human IgG subclasses (IgG1, IgG2, IgG3, and IgG4) were used. The total IgG anti-H. pylori antibody titres were equal in all three H. pylori-positive groups and significantly different from that of the negative control group (p less than 0.01). The IgG subclass response in persons infected with H. pylori involved all four subclasses but was predominantly of the IgG1 and IgG2 subclasses. All of the groups with H. pylori infection had significantly higher levels of IgG1 than the negative control group, but no differences were detected among the three groups. However, the duodenal ulcer group had a significantly higher IgG2 response than the gastritis group (mean optical density +/- SEM, 0.382 +/- 0.047 versus 0.200 +/- 0.025, respectively; p less than 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)

KW - Adult

KW - Aged

KW - Chronic Disease

KW - Duodenal Ulcer/blood

KW - Female

KW - Gastritis/blood

KW - Helicobacter pylori/immunology

KW - Humans

KW - Immunoglobulin G/blood

KW - Male

KW - Middle Aged

M3 - Article

VL - 27

SP - 129

EP - 133

JO - Scandinavian Journal of Gastroenterology

JF - Scandinavian Journal of Gastroenterology

SN - 0036-5521

IS - 2

ER -