TY - JOUR
T1 - Impact of delayed and prolonged fixation on the evaluation of immunohistochemical staining on lung carcinoma resection specimen
AU - van Seijen, Maartje
AU - Brcic, Luka
AU - Gonzales, Atilio Navarro
AU - Sansano, Irene
AU - Bendek, Matyas
AU - Brcic, Iva
AU - Lissenberg-Witte, Birgit
AU - Korkmaz, H. Ibrahim
AU - Geiger, Thomas
AU - Kammler, Rosita
AU - Stahel, Rolf
AU - Thunnissen, Erik
AU - On behalf of ETOP
PY - 2019/8/1
Y1 - 2019/8/1
N2 - Pre-analytical factors, such as fixation time, influence morphology of diagnostic and predictive immunohistochemical staining, which are increasingly used in the evaluation of lung cancer. Our aim was to investigate if variations in fixation time influence the outcome of immunohistochemical staining in lung cancer. From lung resections, specimen with tumor size bigger than 4 cm, 10 samples were obtained: 2 were put through the standard fixation protocol, 5 through the delayed, and 3 through the prolonged fixation protocol. After paraffin embedding, tissue microarrays (TMAs) were made. They were stained with 20 antibodies and scored for quality and intensity of staining. Samples with delay in fixation showed loss of TMA cores on glass slides and deterioration of tissue quality leading to reduction in the expression of CK 7, Keratin MNF116, CAM 5.2, CK 5/6, TTF-1, C-MET, Napsin A, D2-40, and PD-L1. Prolonged fixation had no influence on the performance of immunohistochemical stains. Delay of fixation negatively affects the expression of different immunohistochemical markers, influencing diagnostic (cytokeratins) and predictive (PD-L1) testing. These results emphasize the need for adequate fixation of resection specimen.
AB - Pre-analytical factors, such as fixation time, influence morphology of diagnostic and predictive immunohistochemical staining, which are increasingly used in the evaluation of lung cancer. Our aim was to investigate if variations in fixation time influence the outcome of immunohistochemical staining in lung cancer. From lung resections, specimen with tumor size bigger than 4 cm, 10 samples were obtained: 2 were put through the standard fixation protocol, 5 through the delayed, and 3 through the prolonged fixation protocol. After paraffin embedding, tissue microarrays (TMAs) were made. They were stained with 20 antibodies and scored for quality and intensity of staining. Samples with delay in fixation showed loss of TMA cores on glass slides and deterioration of tissue quality leading to reduction in the expression of CK 7, Keratin MNF116, CAM 5.2, CK 5/6, TTF-1, C-MET, Napsin A, D2-40, and PD-L1. Prolonged fixation had no influence on the performance of immunohistochemical stains. Delay of fixation negatively affects the expression of different immunohistochemical markers, influencing diagnostic (cytokeratins) and predictive (PD-L1) testing. These results emphasize the need for adequate fixation of resection specimen.
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85069446674&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/31264038
U2 - 10.1007/s00428-019-02595-9
DO - 10.1007/s00428-019-02595-9
M3 - Article
C2 - 31264038
VL - 475
SP - 191
EP - 199
JO - Virchows Archiv
JF - Virchows Archiv
SN - 0945-6317
IS - 2
ER -