In vivo bladder cancer diagnosis by high-volume Raman spectroscopy

Ronald O.P. Draga, Matthijs C.M. Grimbergen, Peter L.M. Vijverberg, Christiaan F.P.Van Swol, Trudy G.N. Jonges, J. Alain Kummer, J. L.H. Ruud Bosch

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

We studied the feasibility of Raman spectroscopy for the diagnosis of bladder cancer in vivo. Since the invasion stage is crucial for the treatment choice, a high-volume based Raman probe was used to investigate the potential of determining the invasiveness of bladder cancer. High quality spectra were obtained from suspicious and nonsuspicious bladder locations during the procedure of transurethral resection of bladder tumors (TURBT) with collection times of 1-5 s. Multivariate analysis was used to generate the classification models. The algorithm was able to distinguish bladder cancer from normal bladder locations with a sensitivity of 85% and a specificity of 79%. The Raman spectra of bladder cancer stages showed a gradual increase in the intensity of specific amino acid peaks and, most likely, an increase in the intensity of DNA peaks.

Original languageEnglish
Pages (from-to)5993-5999
Number of pages7
JournalAnalytical Chemistry
Volume82
Issue number14
DOIs
Publication statusPublished - 15 Jul 2010

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