Increased expression of DC-SIGN+IL-12+IL-18+ and CD83+IL-12-IL-18- dendritic cell populations in the colonic mucosa of patients with Crohn's disease

Anje A te Velde, Yvette van Kooyk, Henri Braat, Daan W Hommes, Trees A M Dellemijn, J Frederik M Slors, Sander J H van Deventer, Florry A Vyth-Dreese

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Dentritic cells (DC) as antigen-presenting cells are most likely responsible for regulation of abnormal T cell activation in Crohn's disease (CD), a chronic inflammatory bowel disease. We have analyzed the expression of activation and maturation markers on DC in the colon mucosa from patients with CD compared with normal colon, using immunohistochemical techniques. We found two distinct populations of DC present in CD patients: a DC-specific ICAM-3 grabbing non-integrin (DC-SIGN)(+) population that was present scattered throughout the mucosa, and a CD83(+) population that was present in aggregated lymphoid nodules and as single cells in the lamina propria. In normal colon the number of DC-SIGN(+) DC was lower and CD83(+) DC were detected only in very few solitary lymphoid nodules. Co-expression of activation markers and cytokine synthesis was analyzed with three-color confocal laser scanning microscopy analysis. CD80 expression was enhanced on the majority of DC-SIGN(+) DC in CD patients, whereas only a proportion of the CD83(+) DC co-expressed CD80 in CD as well as in normal tissue. Surprisingly, IL-12 and IL-18 were only detected in DC-SIGN(+) DC and not in CD83(+) DC. A similar pattern of cytokine production was observed in normal colon albeit to a much lesser extent. The characteristics of these in-situ-differentiated DC markedly differ from the in-vitro-generated DC that simultaneously express DC-SIGN, CD83 and cytokines.

Original languageEnglish
Pages (from-to)143-51
Number of pages9
JournalEuropean Journal of Immunology
Volume33
Issue number1
DOIs
Publication statusPublished - Jan 2003

Cite this

te Velde, Anje A ; van Kooyk, Yvette ; Braat, Henri ; Hommes, Daan W ; Dellemijn, Trees A M ; Slors, J Frederik M ; van Deventer, Sander J H ; Vyth-Dreese, Florry A. / Increased expression of DC-SIGN+IL-12+IL-18+ and CD83+IL-12-IL-18- dendritic cell populations in the colonic mucosa of patients with Crohn's disease. In: European Journal of Immunology. 2003 ; Vol. 33, No. 1. pp. 143-51.
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title = "Increased expression of DC-SIGN+IL-12+IL-18+ and CD83+IL-12-IL-18- dendritic cell populations in the colonic mucosa of patients with Crohn's disease",
abstract = "Dentritic cells (DC) as antigen-presenting cells are most likely responsible for regulation of abnormal T cell activation in Crohn's disease (CD), a chronic inflammatory bowel disease. We have analyzed the expression of activation and maturation markers on DC in the colon mucosa from patients with CD compared with normal colon, using immunohistochemical techniques. We found two distinct populations of DC present in CD patients: a DC-specific ICAM-3 grabbing non-integrin (DC-SIGN)(+) population that was present scattered throughout the mucosa, and a CD83(+) population that was present in aggregated lymphoid nodules and as single cells in the lamina propria. In normal colon the number of DC-SIGN(+) DC was lower and CD83(+) DC were detected only in very few solitary lymphoid nodules. Co-expression of activation markers and cytokine synthesis was analyzed with three-color confocal laser scanning microscopy analysis. CD80 expression was enhanced on the majority of DC-SIGN(+) DC in CD patients, whereas only a proportion of the CD83(+) DC co-expressed CD80 in CD as well as in normal tissue. Surprisingly, IL-12 and IL-18 were only detected in DC-SIGN(+) DC and not in CD83(+) DC. A similar pattern of cytokine production was observed in normal colon albeit to a much lesser extent. The characteristics of these in-situ-differentiated DC markedly differ from the in-vitro-generated DC that simultaneously express DC-SIGN, CD83 and cytokines.",
keywords = "Adult, Antigens, CD, Cell Adhesion Molecules/metabolism, Colon/immunology, Crohn Disease/immunology, Dendritic Cells/immunology, Flow Cytometry, Gene Expression, Humans, Immunoglobulins/metabolism, Interleukin-12/analysis, Interleukin-18/analysis, Intestinal Mucosa/immunology, Lectins, C-Type/metabolism, Membrane Glycoproteins/metabolism, Microscopy, Confocal, Middle Aged, Receptors, Cell Surface/metabolism",
author = "{te Velde}, {Anje A} and {van Kooyk}, Yvette and Henri Braat and Hommes, {Daan W} and Dellemijn, {Trees A M} and Slors, {J Frederik M} and {van Deventer}, {Sander J H} and Vyth-Dreese, {Florry A}",
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pages = "143--51",
journal = "European Journal of Immunology",
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Increased expression of DC-SIGN+IL-12+IL-18+ and CD83+IL-12-IL-18- dendritic cell populations in the colonic mucosa of patients with Crohn's disease. / te Velde, Anje A; van Kooyk, Yvette; Braat, Henri; Hommes, Daan W; Dellemijn, Trees A M; Slors, J Frederik M; van Deventer, Sander J H; Vyth-Dreese, Florry A.

In: European Journal of Immunology, Vol. 33, No. 1, 01.2003, p. 143-51.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Increased expression of DC-SIGN+IL-12+IL-18+ and CD83+IL-12-IL-18- dendritic cell populations in the colonic mucosa of patients with Crohn's disease

AU - te Velde, Anje A

AU - van Kooyk, Yvette

AU - Braat, Henri

AU - Hommes, Daan W

AU - Dellemijn, Trees A M

AU - Slors, J Frederik M

AU - van Deventer, Sander J H

AU - Vyth-Dreese, Florry A

PY - 2003/1

Y1 - 2003/1

N2 - Dentritic cells (DC) as antigen-presenting cells are most likely responsible for regulation of abnormal T cell activation in Crohn's disease (CD), a chronic inflammatory bowel disease. We have analyzed the expression of activation and maturation markers on DC in the colon mucosa from patients with CD compared with normal colon, using immunohistochemical techniques. We found two distinct populations of DC present in CD patients: a DC-specific ICAM-3 grabbing non-integrin (DC-SIGN)(+) population that was present scattered throughout the mucosa, and a CD83(+) population that was present in aggregated lymphoid nodules and as single cells in the lamina propria. In normal colon the number of DC-SIGN(+) DC was lower and CD83(+) DC were detected only in very few solitary lymphoid nodules. Co-expression of activation markers and cytokine synthesis was analyzed with three-color confocal laser scanning microscopy analysis. CD80 expression was enhanced on the majority of DC-SIGN(+) DC in CD patients, whereas only a proportion of the CD83(+) DC co-expressed CD80 in CD as well as in normal tissue. Surprisingly, IL-12 and IL-18 were only detected in DC-SIGN(+) DC and not in CD83(+) DC. A similar pattern of cytokine production was observed in normal colon albeit to a much lesser extent. The characteristics of these in-situ-differentiated DC markedly differ from the in-vitro-generated DC that simultaneously express DC-SIGN, CD83 and cytokines.

AB - Dentritic cells (DC) as antigen-presenting cells are most likely responsible for regulation of abnormal T cell activation in Crohn's disease (CD), a chronic inflammatory bowel disease. We have analyzed the expression of activation and maturation markers on DC in the colon mucosa from patients with CD compared with normal colon, using immunohistochemical techniques. We found two distinct populations of DC present in CD patients: a DC-specific ICAM-3 grabbing non-integrin (DC-SIGN)(+) population that was present scattered throughout the mucosa, and a CD83(+) population that was present in aggregated lymphoid nodules and as single cells in the lamina propria. In normal colon the number of DC-SIGN(+) DC was lower and CD83(+) DC were detected only in very few solitary lymphoid nodules. Co-expression of activation markers and cytokine synthesis was analyzed with three-color confocal laser scanning microscopy analysis. CD80 expression was enhanced on the majority of DC-SIGN(+) DC in CD patients, whereas only a proportion of the CD83(+) DC co-expressed CD80 in CD as well as in normal tissue. Surprisingly, IL-12 and IL-18 were only detected in DC-SIGN(+) DC and not in CD83(+) DC. A similar pattern of cytokine production was observed in normal colon albeit to a much lesser extent. The characteristics of these in-situ-differentiated DC markedly differ from the in-vitro-generated DC that simultaneously express DC-SIGN, CD83 and cytokines.

KW - Adult

KW - Antigens, CD

KW - Cell Adhesion Molecules/metabolism

KW - Colon/immunology

KW - Crohn Disease/immunology

KW - Dendritic Cells/immunology

KW - Flow Cytometry

KW - Gene Expression

KW - Humans

KW - Immunoglobulins/metabolism

KW - Interleukin-12/analysis

KW - Interleukin-18/analysis

KW - Intestinal Mucosa/immunology

KW - Lectins, C-Type/metabolism

KW - Membrane Glycoproteins/metabolism

KW - Microscopy, Confocal

KW - Middle Aged

KW - Receptors, Cell Surface/metabolism

U2 - 10.1002/immu.200390017

DO - 10.1002/immu.200390017

M3 - Article

VL - 33

SP - 143

EP - 151

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 1

ER -