Induction of a hypersensitive response by chimeric helicase sequences of Tobamoviruses U1 and Ob in N-carrying tobacco

T. E.M. Abbink, J. De Vogel, J. F. Bol, H. J.M. Linthorst*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Recently, the helicase domain of the Tobacco mosaic virus (TMV)-U1 replicase proteins (designated MOREHEL:U1) was identified as the elicitor of the N gene-mediated hypersensitive response (HR) in tobacco. In this study, we used agroinfiltration to express the equivalent MOREHEL domain of the non-HR-inducing tobamovirus strain TMV-Ob. It appeared that this MOREHEL:Ob sequence did not elicit a HR in N gene-carrying tobacco. Both MOREHEL sequences were divided into eight subdomains, and chimeras of MOREHEL sequences from U1 and Ob were constructed. Expression of these chimeric MOREHEL sequences revealed that, in the TMV-U1 MOREHEL sequence, at least four domains involved in full HR induction were present. The presence of at least three of these four domains seems a minimal requirement for HR induction. Two additional domains may play a minor role in HR induction. To study the elicitor function of the chimeras during the TMV life cycle, chimeric MOREHEL domains were introduced into full-length TMV cDNA clones. These constructs, however, were unable to establish an infection in Nicotiana benthamiana or Nicotiana tabacum plants.

Original languageEnglish
Pages (from-to)1086-1095
Number of pages10
JournalMolecular Plant-Microbe Interactions
Volume14
Issue number9
DOIs
Publication statusPublished - 2001

Cite this