TY - JOUR
T1 - Integrated single-cell analysis unveils diverging immune features of covid-19, influenza, and other community-acquired pneumonia
AU - Schuurman, Alex R.
AU - Reijnders, Tom D. Y.
AU - Saris, Anno
AU - Moral, Ivan Ramirez
AU - Schinkel, Michiel
AU - de Brabander, Justin
AU - van Linge, Christine
AU - Vermeulen, Louis
AU - Scicluna, Brendon P.
AU - Wiersinga, W. Joost
AU - Braga, Felipe A. Vieira
AU - van der Poll, Tom
PY - 2021/8/1
Y1 - 2021/8/1
N2 - The exact immunopathophysiology of community-acquired pneumonia (CAP) caused by SARS-CoV-2 (COVID-19) remains clouded by a general lack of relevant disease controls. The scarcity of single-cell investigations in the broader population of patients with CAP renders it difficult to distinguish immune features unique to COVID-19 from the common characteristics of a dysregulated host response to pneumonia. We performed integrated single-cell transcriptomic and proteomic analyses in peripheral blood mononuclear cells from a matched cohort of eight patients with COVID-19, eight patients with CAP caused by Influenza A or other pathogens, and four non-infectious control subjects. Using this balanced, multi-omics approach, we describe shared and diverging transcriptional and phenotypic patterns—including increased levels of type I interferon-stimulated natural killer cells in COVID-19, cytotoxic CD8 T EMRA cells in both COVID-19 and influenza, and distinctive monocyte compositions between all groups—and thereby expand our understanding of the peripheral immune response in different etiologies of pneumonia.
AB - The exact immunopathophysiology of community-acquired pneumonia (CAP) caused by SARS-CoV-2 (COVID-19) remains clouded by a general lack of relevant disease controls. The scarcity of single-cell investigations in the broader population of patients with CAP renders it difficult to distinguish immune features unique to COVID-19 from the common characteristics of a dysregulated host response to pneumonia. We performed integrated single-cell transcriptomic and proteomic analyses in peripheral blood mononuclear cells from a matched cohort of eight patients with COVID-19, eight patients with CAP caused by Influenza A or other pathogens, and four non-infectious control subjects. Using this balanced, multi-omics approach, we describe shared and diverging transcriptional and phenotypic patterns—including increased levels of type I interferon-stimulated natural killer cells in COVID-19, cytotoxic CD8 T EMRA cells in both COVID-19 and influenza, and distinctive monocyte compositions between all groups—and thereby expand our understanding of the peripheral immune response in different etiologies of pneumonia.
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85114094393&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/34424199
U2 - 10.7554/eLife.69661
DO - 10.7554/eLife.69661
M3 - Article
C2 - 34424199
SN - 2050-084X
VL - 10
JO - eLife
JF - eLife
M1 - e69661
ER -