Vitamin-K-dependent carboxylase was prepared from bovine liver, kidney, lung and testis and it was checked that these systems obeyed the laws of normal enzyme kinetics. Four carboxylatable substrates were obtained from different sources and the apparent Michaelis constants of the various carboxylase for these four substrates were measured. From the results thus obrained we conclude that carboxylase is a group name for a number of isoenzymes which are present in heaptic as well as in various non-hepatic tissues.
|Number of pages||4|
|Journal||Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular|
|Publication status||Published - 18 Jul 1985|