Isolation of Naegleria fowleri from a domestic water tank associated with a fatal encephalitis in a 4 month-old Venezuelan child

C. Wagner, M. A. Vethencourt Ysea, M. V. Galindo, C. Guzmán De Rondón, A. J. Nessi Paduani, M. Reyes-Batlle, A. López-Arencibia, I. Sifaoui, M. V. Pérez De Galindo, E. Martínez-Carretero, B. Valladares, S. K. Maciver, J. E. Piñero, J. Lorenzo-Morales

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

This study describes the association of household water system contamination with the pathogenic Free-Living Amoeba (FLA) Naegleria fowleri and a case of fatal Primary Amoebic Meningoencephalitis (PAM) in a child from the state of Monagas in Venezuela. Amoebae were initially identified by microscopy from a sample of cerebrospinal fluid (CSF) from the child. Direct DNA extraction and specific PCR/sequencing for N. fowleri was also carried out from the same CSF sample. In order to determine a possible environmental source of infection, water samples from the water tank of the child’s home and also water bodies recently visited by the child and his family, were examined for the presence of N. fowleri by culture and PCR/sequencing. The results obtained from the collected water samples revealed that only the water tank of the house was positive for N. fowleri. PCR/sequencing showed that the strains isolated from the patient and the water tanks were 100 % identical. Therefore, the house water tank was confirmed as the source of infection in this case, possibly as a result of the occasional immersion of the child´s head under the water while bathing. This case highlights a novel source of thermally polluted water and another threat of N. fowleri infection.

Original languageEnglish
Pages (from-to)332-337
Number of pages6
JournalTropical Biomedicine
Volume34
Issue number2
Publication statusPublished - 2017

Cite this

Wagner, C., Vethencourt Ysea, M. A., Galindo, M. V., Guzmán De Rondón, C., Nessi Paduani, A. J., Reyes-Batlle, M., ... Lorenzo-Morales, J. (2017). Isolation of Naegleria fowleri from a domestic water tank associated with a fatal encephalitis in a 4 month-old Venezuelan child. Tropical Biomedicine, 34(2), 332-337.
Wagner, C. ; Vethencourt Ysea, M. A. ; Galindo, M. V. ; Guzmán De Rondón, C. ; Nessi Paduani, A. J. ; Reyes-Batlle, M. ; López-Arencibia, A. ; Sifaoui, I. ; Pérez De Galindo, M. V. ; Martínez-Carretero, E. ; Valladares, B. ; Maciver, S. K. ; Piñero, J. E. ; Lorenzo-Morales, J. / Isolation of Naegleria fowleri from a domestic water tank associated with a fatal encephalitis in a 4 month-old Venezuelan child. In: Tropical Biomedicine. 2017 ; Vol. 34, No. 2. pp. 332-337.
@article{16fedb258bfb47e6a86f8cc062f96b51,
title = "Isolation of Naegleria fowleri from a domestic water tank associated with a fatal encephalitis in a 4 month-old Venezuelan child",
abstract = "This study describes the association of household water system contamination with the pathogenic Free-Living Amoeba (FLA) Naegleria fowleri and a case of fatal Primary Amoebic Meningoencephalitis (PAM) in a child from the state of Monagas in Venezuela. Amoebae were initially identified by microscopy from a sample of cerebrospinal fluid (CSF) from the child. Direct DNA extraction and specific PCR/sequencing for N. fowleri was also carried out from the same CSF sample. In order to determine a possible environmental source of infection, water samples from the water tank of the child’s home and also water bodies recently visited by the child and his family, were examined for the presence of N. fowleri by culture and PCR/sequencing. The results obtained from the collected water samples revealed that only the water tank of the house was positive for N. fowleri. PCR/sequencing showed that the strains isolated from the patient and the water tanks were 100 {\%} identical. Therefore, the house water tank was confirmed as the source of infection in this case, possibly as a result of the occasional immersion of the child´s head under the water while bathing. This case highlights a novel source of thermally polluted water and another threat of N. fowleri infection.",
author = "C. Wagner and {Vethencourt Ysea}, {M. A.} and Galindo, {M. V.} and {Guzm{\'a}n De Rond{\'o}n}, C. and {Nessi Paduani}, {A. J.} and M. Reyes-Batlle and A. L{\'o}pez-Arencibia and I. Sifaoui and {P{\'e}rez De Galindo}, {M. V.} and E. Mart{\'i}nez-Carretero and B. Valladares and Maciver, {S. K.} and Pi{\~n}ero, {J. E.} and J. Lorenzo-Morales",
year = "2017",
language = "English",
volume = "34",
pages = "332--337",
journal = "Tropical Biomedicine",
issn = "0127-5720",
publisher = "Malaysian Society of Parasitology and Tropical Medicine",
number = "2",

}

Wagner, C, Vethencourt Ysea, MA, Galindo, MV, Guzmán De Rondón, C, Nessi Paduani, AJ, Reyes-Batlle, M, López-Arencibia, A, Sifaoui, I, Pérez De Galindo, MV, Martínez-Carretero, E, Valladares, B, Maciver, SK, Piñero, JE & Lorenzo-Morales, J 2017, 'Isolation of Naegleria fowleri from a domestic water tank associated with a fatal encephalitis in a 4 month-old Venezuelan child' Tropical Biomedicine, vol. 34, no. 2, pp. 332-337.

Isolation of Naegleria fowleri from a domestic water tank associated with a fatal encephalitis in a 4 month-old Venezuelan child. / Wagner, C.; Vethencourt Ysea, M. A.; Galindo, M. V.; Guzmán De Rondón, C.; Nessi Paduani, A. J.; Reyes-Batlle, M.; López-Arencibia, A.; Sifaoui, I.; Pérez De Galindo, M. V.; Martínez-Carretero, E.; Valladares, B.; Maciver, S. K.; Piñero, J. E.; Lorenzo-Morales, J.

In: Tropical Biomedicine, Vol. 34, No. 2, 2017, p. 332-337.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Isolation of Naegleria fowleri from a domestic water tank associated with a fatal encephalitis in a 4 month-old Venezuelan child

AU - Wagner, C.

AU - Vethencourt Ysea, M. A.

AU - Galindo, M. V.

AU - Guzmán De Rondón, C.

AU - Nessi Paduani, A. J.

AU - Reyes-Batlle, M.

AU - López-Arencibia, A.

AU - Sifaoui, I.

AU - Pérez De Galindo, M. V.

AU - Martínez-Carretero, E.

AU - Valladares, B.

AU - Maciver, S. K.

AU - Piñero, J. E.

AU - Lorenzo-Morales, J.

PY - 2017

Y1 - 2017

N2 - This study describes the association of household water system contamination with the pathogenic Free-Living Amoeba (FLA) Naegleria fowleri and a case of fatal Primary Amoebic Meningoencephalitis (PAM) in a child from the state of Monagas in Venezuela. Amoebae were initially identified by microscopy from a sample of cerebrospinal fluid (CSF) from the child. Direct DNA extraction and specific PCR/sequencing for N. fowleri was also carried out from the same CSF sample. In order to determine a possible environmental source of infection, water samples from the water tank of the child’s home and also water bodies recently visited by the child and his family, were examined for the presence of N. fowleri by culture and PCR/sequencing. The results obtained from the collected water samples revealed that only the water tank of the house was positive for N. fowleri. PCR/sequencing showed that the strains isolated from the patient and the water tanks were 100 % identical. Therefore, the house water tank was confirmed as the source of infection in this case, possibly as a result of the occasional immersion of the child´s head under the water while bathing. This case highlights a novel source of thermally polluted water and another threat of N. fowleri infection.

AB - This study describes the association of household water system contamination with the pathogenic Free-Living Amoeba (FLA) Naegleria fowleri and a case of fatal Primary Amoebic Meningoencephalitis (PAM) in a child from the state of Monagas in Venezuela. Amoebae were initially identified by microscopy from a sample of cerebrospinal fluid (CSF) from the child. Direct DNA extraction and specific PCR/sequencing for N. fowleri was also carried out from the same CSF sample. In order to determine a possible environmental source of infection, water samples from the water tank of the child’s home and also water bodies recently visited by the child and his family, were examined for the presence of N. fowleri by culture and PCR/sequencing. The results obtained from the collected water samples revealed that only the water tank of the house was positive for N. fowleri. PCR/sequencing showed that the strains isolated from the patient and the water tanks were 100 % identical. Therefore, the house water tank was confirmed as the source of infection in this case, possibly as a result of the occasional immersion of the child´s head under the water while bathing. This case highlights a novel source of thermally polluted water and another threat of N. fowleri infection.

UR - http://www.scopus.com/inward/record.url?scp=85021253929&partnerID=8YFLogxK

M3 - Article

VL - 34

SP - 332

EP - 337

JO - Tropical Biomedicine

JF - Tropical Biomedicine

SN - 0127-5720

IS - 2

ER -

Wagner C, Vethencourt Ysea MA, Galindo MV, Guzmán De Rondón C, Nessi Paduani AJ, Reyes-Batlle M et al. Isolation of Naegleria fowleri from a domestic water tank associated with a fatal encephalitis in a 4 month-old Venezuelan child. Tropical Biomedicine. 2017;34(2):332-337.