Lactobacillus iners-dominated vaginal microbiota is associated with increased susceptibility to Chlamydia trachomatis infection in Dutch women: A case-control study

Robin van Houdt, Bing Ma, Sylvia M. Bruisten, Arjen G. C. L. Speksnijder, Jacques Ravel, Henry J. C. de Vries

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Introduction: This prospective study aimed to study the composition and structure of the vaginal microbiota prior to Chlamydia trachomatis infection. Methods: A nested case-control study was performed in 122 women, half of which acquired C. trachomatis within a year after their first visit. At the first visit, the composition and structure of vaginal microbial communities were analysed using 16S rRNA sequencing in the context of the sociodemographic and sexual risk behaviour information using logistic regression. Results: Five vaginal community state types (CSTs) were identified. Four CSTs were dominated by Lactobacillus spp., of which L. crispatus (37%) and L. iners (33%) were the most common. One CST was characterised by the absence of Lactobacillus spp. (25%) and the presence of an array of strict and facultative anaerobes. Multivariate logistic regression analysis revealed that women with a L. iners-dominated CST had an increased risk of C. trachomatis infection (p=0.04; OR: 2.6, 95% CI 1.0 to 6.6). Conclusions: The distribution of CSTs dominated by Lactobacillus spp. agreed with previous studies. However, the frequency of dysbiosis among Caucasian women was relatively high (24%). Having vaginal microbiota dominated by L. iners was associated with an increased risk for C. trachomatis infection. Therefore, we hypothesise that specific signatures of vaginal microbiota are indicative of increased host predisposition to acquiring STIs.
Original languageEnglish
Pages (from-to)117-123
JournalSexually Transmitted Infections
Volume94
Issue number2
DOIs
Publication statusPublished - 2018

Cite this

@article{ecf2d514c6e84d708e47ea842aacf299,
title = "Lactobacillus iners-dominated vaginal microbiota is associated with increased susceptibility to Chlamydia trachomatis infection in Dutch women: A case-control study",
abstract = "Introduction: This prospective study aimed to study the composition and structure of the vaginal microbiota prior to Chlamydia trachomatis infection. Methods: A nested case-control study was performed in 122 women, half of which acquired C. trachomatis within a year after their first visit. At the first visit, the composition and structure of vaginal microbial communities were analysed using 16S rRNA sequencing in the context of the sociodemographic and sexual risk behaviour information using logistic regression. Results: Five vaginal community state types (CSTs) were identified. Four CSTs were dominated by Lactobacillus spp., of which L. crispatus (37{\%}) and L. iners (33{\%}) were the most common. One CST was characterised by the absence of Lactobacillus spp. (25{\%}) and the presence of an array of strict and facultative anaerobes. Multivariate logistic regression analysis revealed that women with a L. iners-dominated CST had an increased risk of C. trachomatis infection (p=0.04; OR: 2.6, 95{\%} CI 1.0 to 6.6). Conclusions: The distribution of CSTs dominated by Lactobacillus spp. agreed with previous studies. However, the frequency of dysbiosis among Caucasian women was relatively high (24{\%}). Having vaginal microbiota dominated by L. iners was associated with an increased risk for C. trachomatis infection. Therefore, we hypothesise that specific signatures of vaginal microbiota are indicative of increased host predisposition to acquiring STIs.",
author = "{van Houdt}, Robin and Bing Ma and Bruisten, {Sylvia M.} and Speksnijder, {Arjen G. C. L.} and Jacques Ravel and {de Vries}, {Henry J. C.}",
year = "2018",
doi = "10.1136/sextrans-2017-053133",
language = "English",
volume = "94",
pages = "117--123",
journal = "Sexually Transmitted Infections",
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publisher = "BMJ Publishing Group",
number = "2",

}

Lactobacillus iners-dominated vaginal microbiota is associated with increased susceptibility to Chlamydia trachomatis infection in Dutch women: A case-control study. / van Houdt, Robin; Ma, Bing; Bruisten, Sylvia M.; Speksnijder, Arjen G. C. L.; Ravel, Jacques; de Vries, Henry J. C.

In: Sexually Transmitted Infections, Vol. 94, No. 2, 2018, p. 117-123.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Lactobacillus iners-dominated vaginal microbiota is associated with increased susceptibility to Chlamydia trachomatis infection in Dutch women: A case-control study

AU - van Houdt, Robin

AU - Ma, Bing

AU - Bruisten, Sylvia M.

AU - Speksnijder, Arjen G. C. L.

AU - Ravel, Jacques

AU - de Vries, Henry J. C.

PY - 2018

Y1 - 2018

N2 - Introduction: This prospective study aimed to study the composition and structure of the vaginal microbiota prior to Chlamydia trachomatis infection. Methods: A nested case-control study was performed in 122 women, half of which acquired C. trachomatis within a year after their first visit. At the first visit, the composition and structure of vaginal microbial communities were analysed using 16S rRNA sequencing in the context of the sociodemographic and sexual risk behaviour information using logistic regression. Results: Five vaginal community state types (CSTs) were identified. Four CSTs were dominated by Lactobacillus spp., of which L. crispatus (37%) and L. iners (33%) were the most common. One CST was characterised by the absence of Lactobacillus spp. (25%) and the presence of an array of strict and facultative anaerobes. Multivariate logistic regression analysis revealed that women with a L. iners-dominated CST had an increased risk of C. trachomatis infection (p=0.04; OR: 2.6, 95% CI 1.0 to 6.6). Conclusions: The distribution of CSTs dominated by Lactobacillus spp. agreed with previous studies. However, the frequency of dysbiosis among Caucasian women was relatively high (24%). Having vaginal microbiota dominated by L. iners was associated with an increased risk for C. trachomatis infection. Therefore, we hypothesise that specific signatures of vaginal microbiota are indicative of increased host predisposition to acquiring STIs.

AB - Introduction: This prospective study aimed to study the composition and structure of the vaginal microbiota prior to Chlamydia trachomatis infection. Methods: A nested case-control study was performed in 122 women, half of which acquired C. trachomatis within a year after their first visit. At the first visit, the composition and structure of vaginal microbial communities were analysed using 16S rRNA sequencing in the context of the sociodemographic and sexual risk behaviour information using logistic regression. Results: Five vaginal community state types (CSTs) were identified. Four CSTs were dominated by Lactobacillus spp., of which L. crispatus (37%) and L. iners (33%) were the most common. One CST was characterised by the absence of Lactobacillus spp. (25%) and the presence of an array of strict and facultative anaerobes. Multivariate logistic regression analysis revealed that women with a L. iners-dominated CST had an increased risk of C. trachomatis infection (p=0.04; OR: 2.6, 95% CI 1.0 to 6.6). Conclusions: The distribution of CSTs dominated by Lactobacillus spp. agreed with previous studies. However, the frequency of dysbiosis among Caucasian women was relatively high (24%). Having vaginal microbiota dominated by L. iners was associated with an increased risk for C. trachomatis infection. Therefore, we hypothesise that specific signatures of vaginal microbiota are indicative of increased host predisposition to acquiring STIs.

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DO - 10.1136/sextrans-2017-053133

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EP - 123

JO - Sexually Transmitted Infections

JF - Sexually Transmitted Infections

SN - 1368-4973

IS - 2

ER -