Laser microdissection for spatial proteomics of postmortem brain tissue

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

Abstract

Quantitative data of the proteome is highly valuable for providing unbiased information on protein expression changes related to disease or identifying related biomarkers. In brain diseases the affected area can be small and pathogenic events can be related to a specific cell type in an otherwise heterogeneous tissue type. An emerging approach dedicated to analysing this type of samples is laser microdissection (LMD) combined with LC-MS/MS into a single workflow. In this chapter, we describe two LMD methods for isolating tissue at the level of a small area and individual cells suitable for subsequent LC-MS/MS analysis.

Original languageEnglish
Title of host publicationNeuromethods
PublisherHumana Press Inc.
Pages43-53
Number of pages11
DOIs
Publication statusPublished - 1 Jan 2019

Publication series

NameNeuromethods
Volume146
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

Cite this

@inbook{29ac8973861a4bf28edbfa06704d1c46,
title = "Laser microdissection for spatial proteomics of postmortem brain tissue",
abstract = "Quantitative data of the proteome is highly valuable for providing unbiased information on protein expression changes related to disease or identifying related biomarkers. In brain diseases the affected area can be small and pathogenic events can be related to a specific cell type in an otherwise heterogeneous tissue type. An emerging approach dedicated to analysing this type of samples is laser microdissection (LMD) combined with LC-MS/MS into a single workflow. In this chapter, we describe two LMD methods for isolating tissue at the level of a small area and individual cells suitable for subsequent LC-MS/MS analysis.",
keywords = "Human brain, Immunohistochemistry, Laser capture, Laser microdissection, Mass spectrometry, Postmortem tissue, Proteomics, Single cell",
author = "Hondius, {David C.} and Hoozemans, {Jeroen J.M.} and Rozemuller, {Annemieke J.M.} and Smit, {August B.}",
year = "2019",
month = "1",
day = "1",
doi = "10.1007/978-1-4939-9662-9_4",
language = "English",
series = "Neuromethods",
publisher = "Humana Press Inc.",
pages = "43--53",
booktitle = "Neuromethods",

}

Laser microdissection for spatial proteomics of postmortem brain tissue. / Hondius, David C.; Hoozemans, Jeroen J.M.; Rozemuller, Annemieke J.M.; Smit, August B.

Neuromethods. Humana Press Inc., 2019. p. 43-53 (Neuromethods; Vol. 146).

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

TY - CHAP

T1 - Laser microdissection for spatial proteomics of postmortem brain tissue

AU - Hondius, David C.

AU - Hoozemans, Jeroen J.M.

AU - Rozemuller, Annemieke J.M.

AU - Smit, August B.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Quantitative data of the proteome is highly valuable for providing unbiased information on protein expression changes related to disease or identifying related biomarkers. In brain diseases the affected area can be small and pathogenic events can be related to a specific cell type in an otherwise heterogeneous tissue type. An emerging approach dedicated to analysing this type of samples is laser microdissection (LMD) combined with LC-MS/MS into a single workflow. In this chapter, we describe two LMD methods for isolating tissue at the level of a small area and individual cells suitable for subsequent LC-MS/MS analysis.

AB - Quantitative data of the proteome is highly valuable for providing unbiased information on protein expression changes related to disease or identifying related biomarkers. In brain diseases the affected area can be small and pathogenic events can be related to a specific cell type in an otherwise heterogeneous tissue type. An emerging approach dedicated to analysing this type of samples is laser microdissection (LMD) combined with LC-MS/MS into a single workflow. In this chapter, we describe two LMD methods for isolating tissue at the level of a small area and individual cells suitable for subsequent LC-MS/MS analysis.

KW - Human brain

KW - Immunohistochemistry

KW - Laser capture

KW - Laser microdissection

KW - Mass spectrometry

KW - Postmortem tissue

KW - Proteomics

KW - Single cell

UR - http://www.scopus.com/inward/record.url?scp=85070536637&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-9662-9_4

DO - 10.1007/978-1-4939-9662-9_4

M3 - Chapter

T3 - Neuromethods

SP - 43

EP - 53

BT - Neuromethods

PB - Humana Press Inc.

ER -