Laser microdissection for spatial proteomics of postmortem brain tissue

David C. Hondius*, Jeroen J.M. Hoozemans, Annemieke J.M. Rozemuller, August B. Smit

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review


Quantitative data of the proteome is highly valuable for providing unbiased information on protein expression changes related to disease or identifying related biomarkers. In brain diseases the affected area can be small and pathogenic events can be related to a specific cell type in an otherwise heterogeneous tissue type. An emerging approach dedicated to analysing this type of samples is laser microdissection (LMD) combined with LC-MS/MS into a single workflow. In this chapter, we describe two LMD methods for isolating tissue at the level of a small area and individual cells suitable for subsequent LC-MS/MS analysis.

Original languageEnglish
Title of host publicationNeuromethods
PublisherHumana Press Inc.
Number of pages11
Publication statusPublished - 1 Jan 2019

Publication series

ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

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