Lipid biochemical changes detected in normal appearing white matter of chronic multiple sclerosis by spectral coherent raman imaging

K. W.C. Poon, C. Brideau, R. Klaver, G. J. Schenk, J. J. Geurts, P. K. Stys

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Multiple sclerosis (MS) exhibits demyelination, inflammatory infiltration, axonal degeneration, and gliosis, affecting widespread regions of the central nervous system (CNS). While white matter MS lesions have been well characterized pathologically, evidence indicates that the MS brain may be globally altered, with subtle abnormalities found in grossly normal appearing white matter (NAWM). These subtle changes are difficult to investigate by common methods such as histochemical stains and conventional magnetic resonance imaging. Thus, the prototypical inflammatory lesion likely represents the most obvious manifestation of a more widespread involvement of the CNS. We describe the application of spectral coherent anti-Stokes Raman Scattering (sCARS) microscopy to study such changes in chronic MS tissue particularly in NAWM. Subtle changes in myelin lipid biochemical signatures and intra-molecular disorder of fatty acid acyl chains of otherwise normal-Appearing myelin were detected, supporting the notion that the biochemical involvement of the MS brain is far more extensive than conventional methods would suggest.

Original languageEnglish
Pages (from-to)1586-1595
Number of pages10
JournalChemical Science
Volume9
Issue number6
DOIs
Publication statusPublished - 2018

Cite this

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title = "Lipid biochemical changes detected in normal appearing white matter of chronic multiple sclerosis by spectral coherent raman imaging",
abstract = "Multiple sclerosis (MS) exhibits demyelination, inflammatory infiltration, axonal degeneration, and gliosis, affecting widespread regions of the central nervous system (CNS). While white matter MS lesions have been well characterized pathologically, evidence indicates that the MS brain may be globally altered, with subtle abnormalities found in grossly normal appearing white matter (NAWM). These subtle changes are difficult to investigate by common methods such as histochemical stains and conventional magnetic resonance imaging. Thus, the prototypical inflammatory lesion likely represents the most obvious manifestation of a more widespread involvement of the CNS. We describe the application of spectral coherent anti-Stokes Raman Scattering (sCARS) microscopy to study such changes in chronic MS tissue particularly in NAWM. Subtle changes in myelin lipid biochemical signatures and intra-molecular disorder of fatty acid acyl chains of otherwise normal-Appearing myelin were detected, supporting the notion that the biochemical involvement of the MS brain is far more extensive than conventional methods would suggest.",
author = "Poon, {K. W.C.} and C. Brideau and R. Klaver and Schenk, {G. J.} and Geurts, {J. J.} and Stys, {P. K.}",
year = "2018",
doi = "10.1039/c7sc03992a",
language = "English",
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journal = "Chemical Science",
issn = "2041-6520",
publisher = "Royal Society of Chemistry",
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Lipid biochemical changes detected in normal appearing white matter of chronic multiple sclerosis by spectral coherent raman imaging. / Poon, K. W.C.; Brideau, C.; Klaver, R.; Schenk, G. J.; Geurts, J. J.; Stys, P. K.

In: Chemical Science, Vol. 9, No. 6, 2018, p. 1586-1595.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Lipid biochemical changes detected in normal appearing white matter of chronic multiple sclerosis by spectral coherent raman imaging

AU - Poon, K. W.C.

AU - Brideau, C.

AU - Klaver, R.

AU - Schenk, G. J.

AU - Geurts, J. J.

AU - Stys, P. K.

PY - 2018

Y1 - 2018

N2 - Multiple sclerosis (MS) exhibits demyelination, inflammatory infiltration, axonal degeneration, and gliosis, affecting widespread regions of the central nervous system (CNS). While white matter MS lesions have been well characterized pathologically, evidence indicates that the MS brain may be globally altered, with subtle abnormalities found in grossly normal appearing white matter (NAWM). These subtle changes are difficult to investigate by common methods such as histochemical stains and conventional magnetic resonance imaging. Thus, the prototypical inflammatory lesion likely represents the most obvious manifestation of a more widespread involvement of the CNS. We describe the application of spectral coherent anti-Stokes Raman Scattering (sCARS) microscopy to study such changes in chronic MS tissue particularly in NAWM. Subtle changes in myelin lipid biochemical signatures and intra-molecular disorder of fatty acid acyl chains of otherwise normal-Appearing myelin were detected, supporting the notion that the biochemical involvement of the MS brain is far more extensive than conventional methods would suggest.

AB - Multiple sclerosis (MS) exhibits demyelination, inflammatory infiltration, axonal degeneration, and gliosis, affecting widespread regions of the central nervous system (CNS). While white matter MS lesions have been well characterized pathologically, evidence indicates that the MS brain may be globally altered, with subtle abnormalities found in grossly normal appearing white matter (NAWM). These subtle changes are difficult to investigate by common methods such as histochemical stains and conventional magnetic resonance imaging. Thus, the prototypical inflammatory lesion likely represents the most obvious manifestation of a more widespread involvement of the CNS. We describe the application of spectral coherent anti-Stokes Raman Scattering (sCARS) microscopy to study such changes in chronic MS tissue particularly in NAWM. Subtle changes in myelin lipid biochemical signatures and intra-molecular disorder of fatty acid acyl chains of otherwise normal-Appearing myelin were detected, supporting the notion that the biochemical involvement of the MS brain is far more extensive than conventional methods would suggest.

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U2 - 10.1039/c7sc03992a

DO - 10.1039/c7sc03992a

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SN - 2041-6520

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