Live Tracking of Inter-organ Communication by Endogenous Exosomes In Vivo

Frederik J Verweij, Celine Revenu, Guillaume Arras, Florent Dingli, Damarys Loew, D Michiel Pegtel, Gautier Follain, Guillaume Allio, Jacky G Goetz, Pascale Zimmermann, Philippe Herbomel, Filippo Del Bene, Graça Raposo, Guillaume van Niel

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Extracellular vesicles (EVs) are released by most cell types but providing evidence for their physiological relevance remains challenging due to a lack of appropriate model organisms. Here, we developed an in vivo model to study EV function by expressing CD63-pHluorin in zebrafish embryos. A combination of imaging methods and proteomic analysis allowed us to study biogenesis, composition, transfer, uptake, and fate of individual endogenous EVs. We identified a subpopulation of EVs with exosome features, released in a syntenin-dependent manner from the yolk syncytial layer into the blood circulation. These exosomes are captured, endocytosed, and degraded by patrolling macrophages and endothelial cells in the caudal vein plexus (CVP) in a scavenger receptor- and dynamin-dependent manner. Interference with exosome biogenesis affected CVP growth, suggesting a role in trophic support. Altogether, our work represents a system for studying endogenous EV function in vivo with high spatiotemporal accuracy, demonstrating functional inter-organ communication by exosomes.

Original languageEnglish
Pages (from-to)573-589.e4
JournalDevelopmental Cell
Volume48
Issue number4
DOIs
Publication statusPublished - 25 Feb 2019

Cite this

Verweij, F. J., Revenu, C., Arras, G., Dingli, F., Loew, D., Pegtel, D. M., ... van Niel, G. (2019). Live Tracking of Inter-organ Communication by Endogenous Exosomes In Vivo. Developmental Cell, 48(4), 573-589.e4. https://doi.org/10.1016/j.devcel.2019.01.004