Loading of acute myeloid leukemia cells with poly(I:C) by electroporation

Eva Lion*, Charlotte M. de Winde, Viggo F.I. Van Tendeloo, Evelien L.J.M. Smits

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

In this chapter, we describe the technique of electroporation as an efficient method to load primary leukemic cells with the double-stranded RNA (dsRNA) analogue, polyriboinosinic polyribocytidylic acid (poly(I:C)), and detail on the delicate freezing and thawing procedure of primary leukemic cells.

Electroporation is a non-viral gene transfer method by which short-term pores in the membrane of cells are generated by an electrical pulse, allowing molecules to enter the cell. RNA electroporation, a technique developed in our laboratory, is a widely used and versatile transfection method for efficient introduction of both coding RNA (messenger RNA) and non-coding RNA, e.g., dsRNA and small interfering (siRNA), into mammalian cells. Accurate cell processing and storage of patient material is essential for optimal recovery and quality of the cell product for downstream applications.

Original languageEnglish
Pages (from-to)233-241
Number of pages9
JournalMethods in Molecular Biology
Volume1139
DOIs
Publication statusPublished - 2014
Externally publishedYes

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