TY - JOUR
T1 - Loading of acute myeloid leukemia cells with poly(I:C) by electroporation
AU - Lion, Eva
AU - de Winde, Charlotte M.
AU - Van Tendeloo, Viggo F.I.
AU - Smits, Evelien L.J.M.
PY - 2014
Y1 - 2014
N2 - In this chapter, we describe the technique of electroporation as an efficient method to load primary leukemic cells with the double-stranded RNA (dsRNA) analogue, polyriboinosinic polyribocytidylic acid (poly(I:C)), and detail on the delicate freezing and thawing procedure of primary leukemic cells.Electroporation is a non-viral gene transfer method by which short-term pores in the membrane of cells are generated by an electrical pulse, allowing molecules to enter the cell. RNA electroporation, a technique developed in our laboratory, is a widely used and versatile transfection method for efficient introduction of both coding RNA (messenger RNA) and non-coding RNA, e.g., dsRNA and small interfering (siRNA), into mammalian cells. Accurate cell processing and storage of patient material is essential for optimal recovery and quality of the cell product for downstream applications.
AB - In this chapter, we describe the technique of electroporation as an efficient method to load primary leukemic cells with the double-stranded RNA (dsRNA) analogue, polyriboinosinic polyribocytidylic acid (poly(I:C)), and detail on the delicate freezing and thawing procedure of primary leukemic cells.Electroporation is a non-viral gene transfer method by which short-term pores in the membrane of cells are generated by an electrical pulse, allowing molecules to enter the cell. RNA electroporation, a technique developed in our laboratory, is a widely used and versatile transfection method for efficient introduction of both coding RNA (messenger RNA) and non-coding RNA, e.g., dsRNA and small interfering (siRNA), into mammalian cells. Accurate cell processing and storage of patient material is essential for optimal recovery and quality of the cell product for downstream applications.
KW - Adjuvant
KW - Cancer cells
KW - Electroporation
KW - Patient specific
KW - Poly(I:C)
KW - RNA
UR - http://www.scopus.com/inward/record.url?scp=84909592443&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-0345-0_20
DO - 10.1007/978-1-4939-0345-0_20
M3 - Article
C2 - 24619684
AN - SCOPUS:84909592443
VL - 1139
SP - 233
EP - 241
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
SN - 1064-3745
ER -