Localization and imaging of radiolabelled monoclonal antibody against squamous‐cell carcinoma of the head and neck in tumor‐bearing nude mice

J. J. Quak*, A. J.M. Balm, J. G.P. Brakkee, R. J. Scheper, H. J. Haisma, B. J.M. Braakhuis, C. J.L.M. Meijer, G. B. Snow

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Nude mice carrying human squamous‐cell carcinoma xenografts were given i.v. injections of radiolabelled monoclonal antibodies (MAbs). MAb E 48, which reacts with squamous‐cell carcinomas, was labelled with 131l,while a second control MAb of similar immunoglobulin subclass was labelled with 125l. Both antibodies were injected simultaneously, then the mice were scanned with a gamma camera or their tissues were removed and antibody uptake was calculated as a percentage of the injected dose. Uptake of E 48 reached a peak value of 16%/g on day 3, while uptake of the control antibody was less than 1.8%/g. By 24 hr after injection tumor could be visualized without subtraction techniques. At days 3 and 7, only xenografts were visible on imaging. These findings suggest that E 48 is capable of high specificity in targeting isotopes to squamous‐cell carcinomas in an experimental setting.

Original languageEnglish
Pages (from-to)534-538
Number of pages5
JournalInternational Journal of Cancer
Volume44
Issue number3
DOIs
Publication statusPublished - 1 Jan 1989

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