Macrophages and the activity of high endothelial venules. The effect of interferon-gamma

R E Mebius, H R Hendriks, J Brevé, G Kraal

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Interruption of the afferent lymphatic vessels of rat popliteal lymph nodes led to the disappearance of the monoclonal antibody ED3-reactive subcapsular sinus macrophages within 3 weeks, but had no effect on the ED1+ macrophages in the paracortical area. This disconnection of the afferent lymph flow to the popliteal lymph node also reduced the capacity of high endothelial venules (HEV) to bind lymphocytes and led to a flattening of the HEV. Activating factors or cells in the incoming lymph might be responsible for the maintenance and function of several different cell populations and we therefore wished to determine if the effects of interruption could be restored by injection of recombinant rat interferon-gamma (rIFN-gamma). Injection of rIFN-gamma directly into operated lymph nodes could mediate an apparent increase of ED1+ cells within 24 h but rIFN-gamma could not restore the macrophage subpopulation in the subcapsular sinus, as recognized by monoclonal antibody ED3. Restoration of the decreased binding capacity of HEV could not be observed with the doses and time points tested, suggesting that HEV are a distinct type of endothelium.

Original languageEnglish
Pages (from-to)1615-8
Number of pages4
JournalEuropean Journal of Immunology
Volume20
Issue number7
DOIs
Publication statusPublished - Jul 1990

Cite this

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title = "Macrophages and the activity of high endothelial venules. The effect of interferon-gamma",
abstract = "Interruption of the afferent lymphatic vessels of rat popliteal lymph nodes led to the disappearance of the monoclonal antibody ED3-reactive subcapsular sinus macrophages within 3 weeks, but had no effect on the ED1+ macrophages in the paracortical area. This disconnection of the afferent lymph flow to the popliteal lymph node also reduced the capacity of high endothelial venules (HEV) to bind lymphocytes and led to a flattening of the HEV. Activating factors or cells in the incoming lymph might be responsible for the maintenance and function of several different cell populations and we therefore wished to determine if the effects of interruption could be restored by injection of recombinant rat interferon-gamma (rIFN-gamma). Injection of rIFN-gamma directly into operated lymph nodes could mediate an apparent increase of ED1+ cells within 24 h but rIFN-gamma could not restore the macrophage subpopulation in the subcapsular sinus, as recognized by monoclonal antibody ED3. Restoration of the decreased binding capacity of HEV could not be observed with the doses and time points tested, suggesting that HEV are a distinct type of endothelium.",
keywords = "Animals, Cell Adhesion/immunology, Endothelium/immunology, Endothelium, Lymphatic/immunology, Interferon-gamma/physiology, Lymph Nodes/immunology, Macrophage Activation, Macrophages/immunology, Male, Rats, Rats, Inbred Strains",
author = "Mebius, {R E} and Hendriks, {H R} and J Brev{\'e} and G Kraal",
year = "1990",
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language = "English",
volume = "20",
pages = "1615--8",
journal = "European Journal of Immunology",
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Macrophages and the activity of high endothelial venules. The effect of interferon-gamma. / Mebius, R E; Hendriks, H R; Brevé, J; Kraal, G.

In: European Journal of Immunology, Vol. 20, No. 7, 07.1990, p. 1615-8.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Mebius, R E

AU - Hendriks, H R

AU - Brevé, J

AU - Kraal, G

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N2 - Interruption of the afferent lymphatic vessels of rat popliteal lymph nodes led to the disappearance of the monoclonal antibody ED3-reactive subcapsular sinus macrophages within 3 weeks, but had no effect on the ED1+ macrophages in the paracortical area. This disconnection of the afferent lymph flow to the popliteal lymph node also reduced the capacity of high endothelial venules (HEV) to bind lymphocytes and led to a flattening of the HEV. Activating factors or cells in the incoming lymph might be responsible for the maintenance and function of several different cell populations and we therefore wished to determine if the effects of interruption could be restored by injection of recombinant rat interferon-gamma (rIFN-gamma). Injection of rIFN-gamma directly into operated lymph nodes could mediate an apparent increase of ED1+ cells within 24 h but rIFN-gamma could not restore the macrophage subpopulation in the subcapsular sinus, as recognized by monoclonal antibody ED3. Restoration of the decreased binding capacity of HEV could not be observed with the doses and time points tested, suggesting that HEV are a distinct type of endothelium.

AB - Interruption of the afferent lymphatic vessels of rat popliteal lymph nodes led to the disappearance of the monoclonal antibody ED3-reactive subcapsular sinus macrophages within 3 weeks, but had no effect on the ED1+ macrophages in the paracortical area. This disconnection of the afferent lymph flow to the popliteal lymph node also reduced the capacity of high endothelial venules (HEV) to bind lymphocytes and led to a flattening of the HEV. Activating factors or cells in the incoming lymph might be responsible for the maintenance and function of several different cell populations and we therefore wished to determine if the effects of interruption could be restored by injection of recombinant rat interferon-gamma (rIFN-gamma). Injection of rIFN-gamma directly into operated lymph nodes could mediate an apparent increase of ED1+ cells within 24 h but rIFN-gamma could not restore the macrophage subpopulation in the subcapsular sinus, as recognized by monoclonal antibody ED3. Restoration of the decreased binding capacity of HEV could not be observed with the doses and time points tested, suggesting that HEV are a distinct type of endothelium.

KW - Animals

KW - Cell Adhesion/immunology

KW - Endothelium/immunology

KW - Endothelium, Lymphatic/immunology

KW - Interferon-gamma/physiology

KW - Lymph Nodes/immunology

KW - Macrophage Activation

KW - Macrophages/immunology

KW - Male

KW - Rats

KW - Rats, Inbred Strains

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