Macrophages Promote Axon Regeneration with Concurrent Neurotoxicity

J.C. Gensel, S. Nakamura, Z. Guan, N. van Rooijen, D.P. Ankeny, P.G. Popovich

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Abstract

Activated macrophages can promote regeneration of CNS axons. However, macrophages also release factors that kill neurons. These opposing functions are likely induced simultaneously but are rarely considered together in the same experimental preparation. A goal of this study was to unequivocally document the concurrent neurotoxic and neuroregenerative potential of activated macrophages. To do so, we quantified the length and magnitude of axon growth from enhanced green fluorescent protein-expressing dorsal root ganglion (DRG) neurons transplanted into the spinal cord in relationship to discrete foci of activated macrophages. Macrophages were activated via intraspinal injections of zymosan, a potent inflammatory stimulus known to increase axon growth and cause neurotoxicity. Using this approach, a significant increase in axon growth up to macrophage foci was evident. Within and adjacent to macrophages, DRG and spinal cord axons were destroyed. Macrophage toxicity became more evident when zymosan was injected closer to DRG soma. Under these conditions, DRG neurons were killed or their ability to extend axons was dramatically impaired. The concurrent induction of pro-regenerative and neurotoxic functions in zymosan-activated macrophages (ZAMs) was confirmed in vitro using DRG and cortical neurons. Importantly, the ability of ZAMs to stimulate axon growth was transient; prolonged exposure to factors produced by ZAMs enhanced cell death and impaired axon growth in surviving neurons. Lipopolysaccharide, another potent macrophage activator, elicited a florid macrophage response, but without enhancing axon growth or notable toxicity. Together, these data show that a single mode of activation endows macrophages with the ability to simultaneously promote axon regeneration and cell killing
Original languageEnglish
Pages (from-to)3956-3968
Number of pages13
JournalJournal of Neuroscience
Volume29
Issue number12
DOIs
Publication statusPublished - 2009

Cite this

Gensel, J. C., Nakamura, S., Guan, Z., van Rooijen, N., Ankeny, D. P., & Popovich, P. G. (2009). Macrophages Promote Axon Regeneration with Concurrent Neurotoxicity. Journal of Neuroscience, 29(12), 3956-3968. https://doi.org/10.1523/JNEUROSCI.3992-08.2009
Gensel, J.C. ; Nakamura, S. ; Guan, Z. ; van Rooijen, N. ; Ankeny, D.P. ; Popovich, P.G. / Macrophages Promote Axon Regeneration with Concurrent Neurotoxicity. In: Journal of Neuroscience. 2009 ; Vol. 29, No. 12. pp. 3956-3968.
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Gensel, JC, Nakamura, S, Guan, Z, van Rooijen, N, Ankeny, DP & Popovich, PG 2009, 'Macrophages Promote Axon Regeneration with Concurrent Neurotoxicity' Journal of Neuroscience, vol. 29, no. 12, pp. 3956-3968. https://doi.org/10.1523/JNEUROSCI.3992-08.2009

Macrophages Promote Axon Regeneration with Concurrent Neurotoxicity. / Gensel, J.C.; Nakamura, S.; Guan, Z.; van Rooijen, N.; Ankeny, D.P.; Popovich, P.G.

In: Journal of Neuroscience, Vol. 29, No. 12, 2009, p. 3956-3968.

Research output: Contribution to journalArticleAcademicpeer-review

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Gensel JC, Nakamura S, Guan Z, van Rooijen N, Ankeny DP, Popovich PG. Macrophages Promote Axon Regeneration with Concurrent Neurotoxicity. Journal of Neuroscience. 2009;29(12):3956-3968. https://doi.org/10.1523/JNEUROSCI.3992-08.2009