MALDI-TOF mass spectrometry for differentiation between Streptococcus pneumoniae and Streptococcus pseudopneumoniae

Joffrey van Prehn, Suzanne Q. van Veen, Jacqueline J.G. Schelfaut, Els Wessels

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

We compared the Vitek MS and Microflex MALDI-TOF mass spectrometry platform for species differentiation within the Streptococcus mitis group with PCR assays targeted at lytA, Spn9802, and recA as reference standard. The Vitek MS correctly identified 10/11 Streptococcus pneumoniae, 13/13 Streptococcus pseudopneumoniae, and 12/13 S. mitis/oralis. The Microflex correctly identified 9/11 S. pneumoniae, 0/13 S. pseudopneumoniae, and 13/13 S. mitis/oralis. MALDI-TOF is a powerful tool for species determination within the mitis group. Diagnostic accuracy varies depending on platform and database used.

Original languageEnglish
Pages (from-to)9-11
Number of pages3
JournalDiagnostic microbiology and infectious disease
Volume85
Issue number1
DOIs
Publication statusPublished - 1 May 2016

Cite this

van Prehn, Joffrey ; van Veen, Suzanne Q. ; Schelfaut, Jacqueline J.G. ; Wessels, Els. / MALDI-TOF mass spectrometry for differentiation between Streptococcus pneumoniae and Streptococcus pseudopneumoniae. In: Diagnostic microbiology and infectious disease. 2016 ; Vol. 85, No. 1. pp. 9-11.
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keywords = "Diagnostic accuracy, MALDI-TOF, Mass spectrometry, Mitis group, PCR, Streptococcus",
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MALDI-TOF mass spectrometry for differentiation between Streptococcus pneumoniae and Streptococcus pseudopneumoniae. / van Prehn, Joffrey; van Veen, Suzanne Q.; Schelfaut, Jacqueline J.G.; Wessels, Els.

In: Diagnostic microbiology and infectious disease, Vol. 85, No. 1, 01.05.2016, p. 9-11.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - MALDI-TOF mass spectrometry for differentiation between Streptococcus pneumoniae and Streptococcus pseudopneumoniae

AU - van Prehn, Joffrey

AU - van Veen, Suzanne Q.

AU - Schelfaut, Jacqueline J.G.

AU - Wessels, Els

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AB - We compared the Vitek MS and Microflex MALDI-TOF mass spectrometry platform for species differentiation within the Streptococcus mitis group with PCR assays targeted at lytA, Spn9802, and recA as reference standard. The Vitek MS correctly identified 10/11 Streptococcus pneumoniae, 13/13 Streptococcus pseudopneumoniae, and 12/13 S. mitis/oralis. The Microflex correctly identified 9/11 S. pneumoniae, 0/13 S. pseudopneumoniae, and 13/13 S. mitis/oralis. MALDI-TOF is a powerful tool for species determination within the mitis group. Diagnostic accuracy varies depending on platform and database used.

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KW - MALDI-TOF

KW - Mass spectrometry

KW - Mitis group

KW - PCR

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